Imaging of cerebellar surface activation In vivo using voltage sensitive dyes

Kim, J.H.; Dunn, M.B.; Hua, Yang; Rydberg, John; Yae, H.; Elias, Stephen A.; Ebner, Timothy J.

doi:10.1016/0306-4522(89)90427-2

Cited by 20 publications

(6 citation statements)

References 27 publications

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“…17 To analyze electrical activity in explants, changes in membrane potential were detected using the voltage-sensitive dye RH237. 18 Explants were loaded with the dye and changes in the intensity of fluorescence signals across the upper urinary tract were recorded over time. Results of these studies showed that spontaneous electrical excitation initiates at the PKJ and propagates distally in a coordinated wave-like manner ( n = 15) (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

The pelvis–kidney junction contains HCN3, a hyperpolarization-activated cation channel that triggers ureter peristalsis

Hurtado

Bub

Herzlinger

2010

Kidney International

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Peristaltic waves of the ureteric smooth muscles move urine down from the kidney, a process that is commonly defective in congenital diseases. To study the mechanisms that control the initiation and direction of contractions, we used video microscopy and optical mapping techniques and found that electrical and contractile waves began in a region where the renal pelvis joined the connective tissue core of the kidney. Separation of this pelvis–kidney junction from more distal urinary tract segments prevented downstream peristalsis, indicating that it housed the trigger for peristalsis. Moreover, cells in the pelvis–kidney junction were found to express isoform 3 of the hyperpolarization-activated cation on channel family known to be required for initiating electrical activity in the brain and heart. Immunocytochemical and real-time PCR analyses found that hyperpolarization-activated cation-3 is expressed at the pelvis–kidney junction where electrical excitation and contractile waves originate. Inhibition of this channel caused a loss of electrical activity at the pelvis–kidney junction and randomized the origin of electrical activity in the urinary tract, thus markedly perturbing contractions. Collectively, our study demonstrates that hyperpolarization-activated cation-3 channels play a fundamental role in coordinating proximal-to-distal peristalsis of the upper urinary tract. This provides insight into the genetic causes of common inherited urinary tract disorders such as reflux and obstruction.

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Section: Resultsmentioning

confidence: 99%

The pelvis–kidney junction contains HCN3, a hyperpolarization-activated cation channel that triggers ureter peristalsis

Hurtado

Bub

Herzlinger

2010

Kidney International

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“…This technique permits us to measure physiological activity with submillisecond resolution from a two-dimensional sheet of cortical tissue during afferent stimulation. Fluorescent optical recordings from Cb have been reported in vivo (Chen et al 1996(Chen et al , 1998; neutral red fluorescence, Kim et al 1989; flavoprotein autofluorescence, Reinert et al 2004) and in vitro (fluorescent voltage-sensitive dyes in rodents, Arata and Ito 2004;Yarom 1999, 2000b). Here, the entire turtle brain was stained in voltage-sensitive absorbance dye and then absorbance changes in transilluminated light were measured (Konnerth et al 1987).…”

Section: Introductionmentioning

confidence: 99%

Topography and Response Timing of Intact Cerebellum Stained With Absorbance Voltage-Sensitive Dye

Brown¹,

Ariel²

2009

Journal of Neurophysiology

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“…Optical imaging of voltage-sensitive dyes, pHsensitive dyes, or intrinsic optical signals seems to be an appropriate method. Indeed, previous imaging studies of cerebellar activity have shown that surface stimulation generates activity that propagates along a beam of parallel fibers (9)(10)(11)(12)(13). By using a pH-sensitive dye, Ebner (12) has shown in vivo that the response to surface stimulation is a beam of activity, whereas the response to face stimulation is organized in parasagittal bands.…”

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confidence: 99%

Patches of synchronized activity in the cerebellar cortex evoked by mossy-fiber stimulation: Questioning the role of parallel fibers

Cohen

Yarom

1998

Proc. Natl. Acad. Sci. U.S.A.

125

103

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The discrepancy between the structural longitudinal organization of the parallel-fiber system in the cerebellar cortex and the functional mosaic-like organization of the cortex has provoked controversial theories about the f low of information in the cerebellum. We address this issue by characterizing the spatiotemporal organization of neuronal activity in the cerebellar cortex by using optical imaging of voltage-sensitive dyes in isolated guinea-pig cerebellum. Parallel-fiber stimulation evoked a narrow beam of activity, which propagated along the parallel fibers. Stimulation of the mossy fibers elicited a circular, nonpropagating patch of synchronized activity. These results strongly support the hypothesis that a beam of parallel fibers, activated by a focal group of granule cells, fails to activate the Purkinje cells along most of its length. It is thus the ascending axon of the granule cell, and not its parallel branches, that activates and defines the basic functional modules of the cerebellar cortex.The cerebellar cortex is composed of five types of neurons organized in a lattice-like structure that receives input from two sources: the mossy fibers and the climbing fibers. Briefly, the axon of each granular cell ascends through the molecular layer, forming several synaptic contacts with Purkinje cells and other cortical interneurons. The axons then bifurcate at various levels within this layer and run longitudinally along the cerebellar folium in a mediolateral direction. These bifurcating axons collectively form the parallel-fiber system, which is oriented perpendicularly to the plane of Purkinje-cell dendrites. As they cross the dendrites of the Purkinje cells, each of these fibers establishes a single, rarely a double, synaptic contact with Purkinje cells along their path (1, 2). Accordingly, the classical view of the functional organization of the cerebellar cortex asserts that the information coming from mossy fibers flows along the parallel fibers (3-5), generating an elongated band of Purkinje-cell activity underneath the parallel-fiber beam. However, peripheral tactile stimulation yielded a contradictory result; patch-like receptive fields were observed (6). Consequently, a modern view of the cerebellar organization has been proposed (7,8). This modern view postulates a radial organization of the cerebellar cortex rather than a mediolateral organization. The radial organization emphasizes the strong input from the ascending branch of the granular-cell axon relative to its parallel branches.To discriminate between the patterns of activity that stem from the two different functional organizations, one should use a technique that enables simultaneous recording from many sites. Optical imaging of voltage-sensitive dyes, pHsensitive dyes, or intrinsic optical signals seems to be an appropriate method. Indeed, previous imaging studies of cerebellar activity have shown that surface stimulation generates activity that propagates along a beam of parallel fibers (9-13). By using a pH-sensitive dye, E...

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Imaging of cerebellar surface activation In vivo using voltage sensitive dyes

Cited by 20 publications

References 27 publications

The pelvis–kidney junction contains HCN3, a hyperpolarization-activated cation channel that triggers ureter peristalsis

The pelvis–kidney junction contains HCN3, a hyperpolarization-activated cation channel that triggers ureter peristalsis

Topography and Response Timing of Intact Cerebellum Stained With Absorbance Voltage-Sensitive Dye

Patches of synchronized activity in the cerebellar cortex evoked by mossy-fiber stimulation: Questioning the role of parallel fibers

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