Imaging of lipid species by MALDI mass spectrometry

Murphy, Robert C.; Hankin, Joseph A.; Barkley, Robert M.

doi:10.1194/jlr.r800051-jlr200

Cited by 269 publications

(253 citation statements)

References 18 publications

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“…The FA detected and here described as masses specific to the OA condition, could be part of these lipids. Future parallel studies using MALDI-imaging for instance, could discriminate these intact lipids as other authors have shown (33)(34)(35).…”

Section: Pca Revealed the Presence Of Droplets Rich In Lipids In Oa Cmentioning

confidence: 94%

Time-of-Flight Secondary Ion Mass Spectrometry-Based Molecular Distribution Distinguishing Healthy and Osteoarthritic Human Cartilage

et al. 2012

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ABSTRACT. Osteoarthritis (OA) is a pathology that ultimately causes joint destruction.The cartilage is one of the principal affected tissues. Alterations in the lipid mediators and an imbalance in the metabolism of cells that form the cartilage (chondrocytes), have been described as contributors to the OA development. In this study, we have studied the distribution of lipids and chemical elements in healthy and OA human cartilage. Time of flight secondary ion mass spectrometry (TOF-SIMS) allows us to study the spatial distribution of molecules at a high resolution on a tissue section. TOF-SIMS revealed a specific peak profile that distinguishes healthy from OA cartilages. The spatial distribution of cholesterol-related peaks exhibited a remarkable difference between healthy and OA cartilages. A distinctive co-localization of cholesterol and other lipids in the superficial area of the cartilage was found. A higher intensity of oleic acid and other fatty acids in the OA cartilages exhibited a similar localization. On the other hand, CNwas observed with a higher intensity in the healthy samples. Finally, we observed an accumulation of calcium and phosphate ions exclusively in areas surrounding the chondrocyte in OA tissues. To our knowledge, this is the first time that TOF-SIMS revealed combined changes in the molecular distribution in the OA human cartilage.

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Section: Pca Revealed the Presence Of Droplets Rich In Lipids In Oa Cmentioning

confidence: 94%

Time-of-Flight Secondary Ion Mass Spectrometry-Based Molecular Distribution Distinguishing Healthy and Osteoarthritic Human Cartilage

et al. 2012

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“…A significant advantage of imaging MS is acquiring chemical information in situ. However, significant tissue preparation is often required that can adversely affect quantitative accuracy and compositional distribution (23). For example, the quantitative accuracy of MALDI-MS and TOF-SIMS is significantly affected by sample preparation necessary to withstand vacuum pressure and ion generation energies (25).…”

Section: Discussionmentioning

confidence: 99%

Visualization of Lipid Droplet Composition by Direct Organelle Mass Spectrometry

Horn

Ledbetter

James

et al. 2011

Journal of Biological Chemistry

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An expanding appreciation for the varied functions of neutral lipids in cellular organisms relies on a more detailed understanding of the mechanisms of lipid production and packaging into cytosolic lipid droplets (LDs). Conventional lipid profiling procedures involve the analysis of tissue extracts and consequently lack cellular or subcellular resolution. Here, we report an approach that combines the visualization of individual LDs, microphase extraction of lipid components from droplets, and the direct identification of lipid composition by nanospray mass spectrometry, even to the level of a single LD. The triacylglycerol (TAG) composition of LDs from several plant sources (mature cotton (Gossypium hirsutum) embryos, roots of cotton seedlings, and Arabidopsis thaliana seeds and leaves) were examined by direct organelle mass spectrometry and revealed the heterogeneity of LDs derived from different plant tissue sources. The analysis of individual LDs makes possible organellar resolution of molecular compositions and will facilitate new studies of LD biogenesis and functions, especially in combination with analysis of morphological and metabolic mutants. Furthermore, direct organelle mass spectrometry could be applied to the molecular analysis of other subcellular compartments and macromolecules. LDs4 are organelles that are specialized for the storage of neutral lipids and as such provide energy-rich reserves in all cellular organisms (1). Understanding LD ontogeny is of major importance to human physiology; on the one hand, seed oils, packaged in LDs, make up a growing proportion of daily caloric intake in most diets around the world, and on the other hand, the regulation of lipid storage and mobilization underlies significant human health issues: obesity, diabetes and cardiovascular disease.Although storage is considered the principal role of neutral lipids, LDs in nonfat storing tissues recently have become more appreciated for their dynamic nature and functional roles independent of storage. These roles include acyl reserves for phospholipid recycling (2), lipid signaling (3), membrane trafficking (2, 4), inflammation and cancer (5), and hostpathogen interactions (6, 7). These various functions attributed to LDs vary with cell type and likely are manifested by differences in droplet composition. The basic structural model of LDs in plant seeds provides a thermodynamically stable organization that is thought to be conserved throughout eukaryotes, although the nature of the lipids and proteins associated with droplets varies with cell/tissue type. The structure describes a neutral lipid core (triacylglycerols in plant seeds and/or steryl esters in other organisms or cell types) surrounded by a phospholipid monolayer with specific proteins associated with the LD surface (8). Although the endoplasmic reticulum is considered by most to be the major cellular location for LD biogenesis, droplets associate frequently with other subcellular compartments, presumably to carry out unique functions (9).The recent emphasis...

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“…Phospholipids are the most abundant lipid constituents, are widely distributed in the brain, and are readily ionized and detected in MALDI MS experiments. Several studies have already reported the distribution of the commonly known phospholipids within the anatomical regions of the brain in fresh tissue sections [16,20,22,24,46,47,51,[54][55][56][71][72][73].…”

Section: Introductionmentioning

confidence: 99%

Imaging of Phospholipids in Formalin Fixed Rat Brain Sections by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry

Carter

McLeod

Bunch

2011

J. Am. Soc. Mass Spectrom.

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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a valuable tool for the analysis of molecules directly from tissue. Imaging of phospholipids is gaining widespread interest, particularly as these lipids have been implicated in a variety of pathologic processes. Formalin fixation (FF) is the standard protocol used in histology laboratories worldwide to preserve tissue for analysis, in order to aid in the diagnosis and prognosis of diseases. This study assesses MALDI imaging of phospholipids directly in formalin fixed tissue, with a view to future analysis of archival tissue. This investigation proves the viability of MALDI-MSI for studying the distribution of lipids directly in formalin fixed tissue, without any pretreatment protocols. High quality molecular images for several phosphatidylcholine (PC) and sphingomyelin (SM) species are presented. Images correspond well with previously published data for the analysis of lipids directly from freshly prepared tissue. Different ionization pathways are observed when analyzing fixed tissue compared with fresh, and this change was found to be associated with formalin buffers employed in fixation protocols. The ability to analyze lipids directly from formalin fixed tissue opens up new doors in the investigation of disease profiles. Pathologic specimens taken for histologic investigation can be analyzed by MALDI-MS to provide greater information on the involvement of lipids in diseased tissue.

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Imaging of lipid species by MALDI mass spectrometry

Cited by 269 publications

References 18 publications

Time-of-Flight Secondary Ion Mass Spectrometry-Based Molecular Distribution Distinguishing Healthy and Osteoarthritic Human Cartilage

Time-of-Flight Secondary Ion Mass Spectrometry-Based Molecular Distribution Distinguishing Healthy and Osteoarthritic Human Cartilage

Visualization of Lipid Droplet Composition by Direct Organelle Mass Spectrometry

Imaging of Phospholipids in Formalin Fixed Rat Brain Sections by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry

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