Imaging of tissue microstructures using a multimodal microscope design

Demos, Stavros G.; Lieber, Chad A.; Lin, Bevin; Ramsamooj, Rajendra

doi:10.1109/jstqe.2005.857385

Cited by 14 publications

(9 citation statements)

References 24 publications

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“…Recently, the use of hyperspectral (reflectance) imaging has gained interest in the analysis of microscopic structures, including microbes, tissues and associated constituents (Schultz et al , 2001; Demos et al , 2005; Demos, 2007; Haaland et al , 2007; Li et al , 2007). A review of recent literature has shown diverse application of short‐ and long‐wave microspectroscopy for the study of bacteria, cancer cells, human tissue morphology and general cellular structural analysis, but not for differentiating viable from non‐viable endospores.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of live‐viable versus dead bacterial endospores by calibrated hyperspectral reflectance microscopy

Anderson

Reynolds

Ringelberg

et al. 2008

Journal of Microscopy

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SummaryThis paper describes the use of hyperspectral imaging microscopy (HIM) for the characterization and differentiation of live viable versus dead/non-viable bacterial endospores for two species of Bacillus. To accomplish this, endosporeforming Bacillus were cultured and differentiated into endospores. Non-viable endospores were produced using sporicidal methods representing standard decontamination procedures incorporating chlorine and peroxide. Finally, endospore samples were lyophilized to prepare them for spectral analysis. Prior to HIM, baseline spectral reflectance characterizing the endospores was measured using an ASD (400-900 nm) reflectance spectrometer. These data were used to calibrate the resulting spectral image data. HIM data comprising 32 images ranging from 400 to 720 nm (visible to near infrared) were recorded using a C-mounted VariSpec hyperspectral camera attached to an epifluorescent microscope. The images produced by the system record the reflectance and absorption features of endospores based on the structure of the outer coat. Analysis of the HIM data was performed using accepted image and spectral processing routines. Where peroxide was the sporicide, changes in the outer endospore coat contributed to structurally significant visible and near infrared signature differences between liveviable versus dead, non-viable endospores. A statistical test for divergence, a method for scoring spectral structural diversity, also showed the difference between viable and non-viable peroxide killed endospores to be statistically significant. These findings may lead to an improved optical procedure to rapidly identify viable and non-viable endospores in situations of decontamination.

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Section: Introductionmentioning

confidence: 99%

Differentiation of live‐viable versus dead bacterial endospores by calibrated hyperspectral reflectance microscopy

Anderson

Reynolds

Ringelberg

et al. 2008

Journal of Microscopy

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“…29 In brief, a compact diode-pumped solid-state laser operating at 266 nm (Intelite, Minden, NV) was used as the photoexcitation source to generate the AF images. The ex vivo images under 266-nm excitation were acquired using a 5 second exposure time with an approximate dose of 30 mJ∕cm 2 in order to optimize baseline image quality.…”

Section: Optical Imaging Protocolmentioning

confidence: 99%

Establishment of rules for interpreting ultraviolet autofluorescence microscopy images for noninvasive detection of Barrett’s esophagus and dysplasia

et al. 2012

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Abstract. The diagnostic potential of autofluorescence (AF) microscopy under ultraviolet (UV) excitation is explored using ex vivo human specimens. The aim is to establish optical patterns (the rules for interpretation) that correspond to normal and abnormal histologies of the esophagus, spanning from early benign modifications (Barrett's esophagus) to subsequent dysplastic change and progression toward carcinoma. This was achieved by developing an image library categorized by disease progression. We considered morphological changes of disease as they are compared with histological diagnosis of the pathological specimen, as well as control samples of normal esophagus, proximal stomach, and small intestine tissue. Our experimental results indicate that UV AF microscopy could provide real-time histological information for visualizing changes in tissue microstructure that are currently undetectable using conventional endoscopic methods.

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“…Tissue was covered with a quartz slide and kept moist with phosphate buffered saline solution during imaging experiments. Ex vivo specimens were imaged without any tissue preparation using a prototype microscope-imaging platform detailed in a previous work 19 .…”

Section: Investigation Of the Origin Of Image Formation And Contrastmentioning

confidence: 99%

Translating ultraviolet autofluorescence microscopy toward clinical endomicroscopy

et al. 2011

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The potential of autofluorescence microscopy under ultraviolet excitation is investigated as a method to visualize superficial epithelial microstructures and their modification with progression of disease. This method does not require the use of contrast agents, sectioning methods, or tissue preparation. Imaging of esophagus tissue is the focus of this study and deals with three main issues: a) What is the origin of the signal; b) How the gradual microstructure modification associated with various stages of esophageal disease is visualized; c) What are the designing parameters for in vivo implementation.

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Imaging of tissue microstructures using a multimodal microscope design

Cited by 14 publications

References 24 publications

Differentiation of live‐viable versus dead bacterial endospores by calibrated hyperspectral reflectance microscopy

Differentiation of live‐viable versus dead bacterial endospores by calibrated hyperspectral reflectance microscopy

Establishment of rules for interpreting ultraviolet autofluorescence microscopy images for noninvasive detection of Barrett’s esophagus and dysplasia

Translating ultraviolet autofluorescence microscopy toward clinical endomicroscopy

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