Imaging of Total Calcium in Urediospore Germlings of Uromyces by Ion Microscopy

Chandra, Subhash; Leinhos, G.; Morrison, George H.; Hoch, H. C.

doi:10.1006/fgbi.1999.1135

Cited by 11 publications

(9 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ca 2؉ imaging. Fluo-3/AM (Biotium, Hayward, Calif.; hereafter referred to as Fluo-3) was used as a Ca 2ϩ -specific probe to assess the cytoplasmic calcium in C. nicotianae (7). C. nicotianae was grown on PDA plates with and without Ca 2ϩ / CaM antagonists under light for 5 to 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…Since all Ca 2ϩ /CaM antagonists were developed originally in mammalian cells, their specificities in C. nicotianae were examined by using Fluo-3 fluorescent dye as a Ca 2ϩ indicator to estimate the cytoplasmic Ca 2ϩ content (7). Fluo-3 is a nonfluorescent dye but produces green fluorescence at wavelengths of 506 to 520 nm after Ca 2ϩ binding.…”

Section: Vol 69 2003mentioning

confidence: 99%

See 1 more Smart Citation

Involvement of Calcium/Calmodulin Signaling in Cercosporin Toxin Biosynthesis byCercosporanicotianae

Chung

2003

Appl Environ Microbiol

View full text Add to dashboard Cite

Cercosporin is a non-host-selective, perylenequinone toxin produced by many phytopathogenic Cercospora species. The involvement of Ca 2؉ /calmodulin (CaM) signaling in cercosporin biosynthesis was investigated by using pharmacological inhibitors. The results suggest that maintaining endogenous Ca 2؉ homeostasis is required for cercosporin biosynthesis in Cercospora nicotianae. The addition of excess Ca 2؉ to the medium slightly increased fungal growth but resulted in a reduction in cercosporin production. The addition of Ca

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Vol 69 2003mentioning

confidence: 99%

Involvement of Calcium/Calmodulin Signaling in Cercosporin Toxin Biosynthesis byCercosporanicotianae

Chung

2003

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…To test this hypothesis, a means of assessing changes in [Ca 2ϩ ] c is needed. Fluorescent dyes such as Indo-1, Fura-2, and Fluo-3 have been used successfully in a number of animal systems; however, their use in fungi has been problematic due in part to the lack of consistent loading and their tendency to sequester into organelles (Read et al, 1992;Knight et al, 1993;Chandra et al, 1999). Pressure injection of dextran-conjugated fluorophores into fungal cells has been used to partially overcome entry of the fluorophore into organelles (Parton et al, 1997); however, this approach is not feasible for Phyllosticta conidia due to their small size.…”

mentioning

confidence: 99%

Expression of Recombinant Aequorin as an Intracellular Calcium Reporter in the Phytopathogenic Fungus Phyllosticta ampelicida

Shaw

Козлова

Read

et al. 2001

Fungal Genetics and Biology

View full text Add to dashboard Cite

“…The subsequent removal of water by freeze-drying (Chandra et al, 1999;Grohovaz et al, 1996;Jorgensen and McGuffee, 1987;Pezzati and Grohovaz, 1999) presents no opportunity for diffusion in liquid water. Diffusion during the fluid phases of embedding is unlikely since the resin is hydrophobic, thus not conducive to ion diffusion.…”

Section: Adequacy Of Proceduresmentioning

confidence: 99%

“…The aim of this work was to identify the components of this hypothesized recycling system in N. crassa by localization of Ca 2ϩ at the ultrastructural level by use of two different methods: (a) electron spectroscopic imaging (ESI) (Ottensmeyer and Andrew, 1980) of freeze-dried hyphae (Chandra et al, 1999;Grohovaz et al, 1996;Jorgensen and McGuffee, 1987;Pezzati and Grohovaz, 1999) and (b) pyroantimoniate precipitation (Blanco and Villela, 1998;Probst, 1986;Wick and Hepler, 1982).…”

confidence: 99%