Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators

Juzans, Marie; Cuche, Céline; Bartolo, Vincenzo Di; Alcover, Andrés

doi:10.1016/bs.mcb.2022.07.016

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Introducing polarity-aware cell subtypes improves model performance in predicting survival status across three patient cohorts (table 1). Given that polar expression can indicate antigen engagement during contact with tumor cells, 5 we confirm that polar cells are more likely than uniform cells to be adjacent to tumor cells; conversely, cells with tumor cells as direct neighbors are more likely to be polar. Tumor cells are significantly more likely to neighbor polar than non-polar immune cells (figure 2).…”

supporting

confidence: 59%

1314 Polarity measurements from multiplex imaging suggest immune cell activation

Wu,

Mayer,

Trevino

et al. 2023

Regular and Young Investigator Award Abstracts

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supporting

confidence: 59%

1314 Polarity measurements from multiplex imaging suggest immune cell activation

Wu,

Mayer,

Trevino

et al. 2023

Regular and Young Investigator Award Abstracts

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“…Table 1) . Given that polar expression can indicate antigen engagement during contact with tumor cells 19 , this provides evidence that polarity is a biologically significant biomarker.…”

Section: Resultsmentioning

confidence: 93%

“…CD8, CD4, and CD20 were used as the representative surface biomarkers for each of the three cell types, respectively. These three cells and biomarkers were chosen as they are known in the literature to exhibit polar expression during engagement 19 .…”

Section: Methodsmentioning

confidence: 99%

PEPSI: Polarity measurements from spatial proteomics imaging suggest immune cell engagement

Wu,

Mayer

et al. 2023

Preprint

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Subcellular protein localization is important for understanding functional states of cells, but measuring and quantifying this information can be difficult and typically requires high-resolution microscopy. In this work, we develop a metric to define surface protein polarity from immunofluorescence (IF) imaging data and use it to identify distinct immune cell states within tumor microenvironments. We apply this metric to characterize over two million cells across 600 patient samples and find that cells identified as having polar expression exhibit characteristics relating to tumor-immune cell engagement. Additionally, we show that incorporating these polarity-defined cell subtypes improves the performance of deep learning models trained to predict patient survival outcomes. This method provides a first look at using subcellular protein expression patterns to phenotype immune cell functional states with applications to precision medicine.

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Measuring interaction force between T lymphocytes and their target cells using live microscopy and laminar shear flow chambers

Goyard,

Schneider,

Ljubetic

et al. 2024

Methods in Cell Biology

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Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators

Cited by 3 publications

References 19 publications

1314 Polarity measurements from multiplex imaging suggest immune cell activation

1314 Polarity measurements from multiplex imaging suggest immune cell activation

PEPSI: Polarity measurements from spatial proteomics imaging suggest immune cell engagement

Measuring interaction force between T lymphocytes and their target cells using live microscopy and laminar shear flow chambers

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