Imaging properties of a multi-reflection time-of-flight mass analyzer

Verenchikov, Anatoly N.; Yavor, M.I.

doi:10.1016/j.ijms.2021.116547

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…For the improvement of resolution, research in past few years have focused on the extension of ion transport distance. Methods are developed such as multi‐pass and multi‐flection time‐of‐flight (MR‐TOF‐MS) (Chernushevich et al, 2017; Dziekonski et al, 2017; Fischer & Schweikhard, 2020; Knauer et al, 2019; Richardson & Hoyes, 2015; Verenchikov & Yavor, 2021; Yavor et al, 2018). Improved MR TOF analyzers of 1 m size demonstrates 500,000 resolving power at restricted mass range (Yavor et al, 2018).…”

Section: Instrumentationmentioning

confidence: 99%

Native top‐down mass spectrometry for higher‐order structural characterization of proteins and complexes

Liu

Xia

2022

Mass Spectrometry Reviews

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Progress in structural biology research has led to a high demand for powerful and yet complementary analytical tools for structural characterization of proteins and protein complexes. This demand has significantly increased interest in native mass spectrometry (nMS), particularly native top‐down mass spectrometry (nTDMS) in the past decade. This review highlights recent advances in nTDMS for structural research of biological assemblies, with a particular focus on the extra multi‐layers of information enabled by TDMS. We include a short introduction of sample preparation and ionization to nMS, tandem fragmentation techniques as well as mass analyzers and software/analysis pipelines used for nTDMS. We highlight unique structural information offered by nTDMS and examples of its broad range of applications in proteins, protein‐ligand interactions (metal, cofactor/drug, DNA/RNA, and protein), therapeutic antibodies and antigen‐antibody complexes, membrane proteins, macromolecular machineries (ribosome, nucleosome, proteosome, and viruses), to endogenous protein complexes. The challenges, potential, along with perspectives of nTDMS methods for the analysis of proteins and protein assemblies in recombinant and biological samples are discussed.

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Section: Instrumentationmentioning

confidence: 99%

Native top‐down mass spectrometry for higher‐order structural characterization of proteins and complexes

Liu

Xia

2022

Mass Spectrometry Reviews

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“…Photomultiplier tubes (PMTs), themselves electron multipliers nested behind a photocathode, provide for magnitude longer lifetime by sealing the dynode surfaces and protecting them from the background material of the instrument vacuum. Hybrid photodetectors incorporating both ion conversion to electrons, a scintillator, and an optically coupled PMT thus offer promise as a long-life ion detection solution, 14 though these multiple conversion stages present a challenge for fast time response compared to more conventional methods. The scintillator, while still exposed to the system atmosphere, sees far lower electron currents than the high-gain stage dynodes, limiting the risk of carbon contamination.…”

Section: ■ Introductionmentioning

confidence: 99%

A High Dynamic Range Ion Detector for Multireflection Time-of-Flight Analyzers

Stewart,

Petzoldt,

Shanley

et al. 2024

J. Am. Soc. Mass Spectrom.

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Reflectron-based time-of-flight analyzers rely on subnanosecond detector time response to achieve acceptable resolving power for low−mid-mass, multiple-ion peaks. With the adoption of multireflection analyzers, order of magnitude longer folded ion paths relax restrictions on detector response time, allowing implementation of new technologies that greatly improve dynamic range, detector lifetime, and ion detection efficiency. A detection system is presented, integrated into a multireflection analyzer, that combines 10 keV postacceleration and focal plane correction with a unique BxE focusing, optically coupled detector, preamplification, and dual-channel digitization. Calibration and peak-handling methods are also described. The instrument demonstrated >1 × 10 4 dynamic range in a single shot, > 100k resolving power, and a relative immunity to detector aging.

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A Perspective of Multi‐Reflecting TOF MS

Verenchikov,

Makarov,

Vorobyev

et al. 2024

Mass Spectrometry Reviews

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Time‐of‐flight mass spectrometry (TOF MS) excels in rapid and high‐sensitivity analysis, making it a cornerstone of analytical chemistry. But as sample complexity explodes in omics studies, so does the need for higher resolving power to ensure accurate results. Traditional TOF instruments face a challenge: achieving high resolution often requires a very large instrument. To overcome this limitation, scientists developed alternative designs for TOF analyzers called multi‐pass TOF analyzers (MPT). These MPT analyzers come in two main configurations: multi‐turn (MTT) and multi‐reflecting (MRT). Drawing on the authors’ extensive experience, this review describes two decades of MPT advancements. It highlights the critical development of optimized analyzer designs, tracing the evolution towards mirror‐based MRT instruments, generally providing superior resolution and spatial acceptance compared to MTT. While the manuscript attempts to overview MTT advances, it primarily focuses on MRT technology. Additionally, the review explores the role of orthogonal accelerators and trap pulse converters, comparing their efficiency and the dynamic range limits imposed by space charge effects. By comparing various MRT configurations and commercially available instruments, the review sets out to inform and empower researchers so they can make informed decisions about MRT mass spectrometers.

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Imaging properties of a multi-reflection time-of-flight mass analyzer

Cited by 4 publications

References 16 publications

Native top‐down mass spectrometry for higher‐order structural characterization of proteins and complexes

Native top‐down mass spectrometry for higher‐order structural characterization of proteins and complexes

A High Dynamic Range Ion Detector for Multireflection Time-of-Flight Analyzers

A Perspective of Multi‐Reflecting TOF MS

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