Imaging rhodopsin degeneration
            <i>in vivo</i>
            in a new model of ocular ischemia in living mice

Ren, Jingyi; Chen, Yin-Ching I.; Mackey, Ashley; Liu, Philip K.

doi:10.1096/fj.15-280677

Cited by 4 publications

(2 citation statements)

References 35 publications

(48 reference statements)

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“…[9] Neutravidin conjugated superparamagnetic iron oxide nanoparticle has also been explored as an imaging agent for rhodopsin degeneration. [10] More recently, the avidin-based nanotechnology has found its applications in tissue engineering and cellular regeneration. [11, 12] In one such study, an avidin-biotin system was used to improve osteoblast-like cell adhesion to a highly porous calcium phosphate glass scaffold for bone tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

Jain

Cheng

2017

Journal of Controlled Release

228

286

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Avidin-biotin interaction is one of the strongest non-covalent interactions in the nature. Avidin and its analogues have therefore been extensively utilized as probes and affinity matrices for a wide variety of applications in biochemical assays, diagnosis, affinity purification, and drug delivery. Recently, there has been a growing interest in exploring this non-covalent interaction in nanoscale drug delivery systems for pharmaceutical agents, including small molecules, proteins, vaccines, monoclonal antibodies, and nucleic acids. Particularly, the ease of fabrication without losing the chemical and biological properties of the coupled moieties makes the avidin-biotin system a versatile platform for nanotechnology. In addition, avidin-based nanoparticles have been investigated as diagnosis systems for various tumors and surface antigens. In this review, we will highlight the various fabrication principles and biomedical applications of avidin-based nanoparticles in drug delivery and diagnosis. The structures and biochemical properties of avidin, biotin and their respective analogues will also be discussed.

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Section: Introductionmentioning

confidence: 99%

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

Jain

Cheng

2017

Journal of Controlled Release

228

286

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“…A few previous manganese-enhanced MRI studies used dual coils to image both eyes in rodents, but each eye was scanned sequentially using a single coil at a time due to the absence of detuning circuits, which would double the scan time ( 78 – 80 ). Some studies have also used a single large coil to image both eyes simultaneously with contrast-enhanced MRI, but with relatively low resolutions (>100 µm) that cannot resolve retinal/choroidal layers ( 81 – 84 ). These previous studies also have not incorporated a third transmit coil for blood labeling for ASL, as was used herein.…”

Section: Discussionmentioning

confidence: 99%

Improved MRI methods to quantify retinal and choroidal blood flow applied to a model of glaucoma

Jiang,

Chernoff,

Galenchik-Chan

et al. 2024

Front. Ophthalmol.

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PurposeBlood flow (BF) of the retinal and choroidal vasculatures can be quantitatively imaged using MRI. This study sought to improve methods of data acquisition and analysis for MRI of layer-specific retinal and choroidal BF and then applied this approach to detect reduced ocular BF in a well-established mouse model of glaucoma from both eyes.MethodsQuantitative BF magnetic resonance imaging (MRI) was performed on glaucomatous DBA/2J and normal C57BL/6J mice. Arterial spin labeling MRI was applied to image retinal and choroidal BF using custom-made dual eye coils that could image both eyes during the same scan. Statistics using data from a single eye or two eyes were compared. BF values were calculated using two approaches. The BF rate per quantity of tissue was calculated as commonly done, and the peak BF values of the retinal and choroidal vasculatures were taken. Additionally, the BF rate per retinal surface area was calculated using a new analysis approach to attempt to reduce partial volume and variability by integrating BF over the retinal and choroidal depths.ResultsOcular BF of both eyes could be imaged using the dual coil setup without effecting scan time. Intraocular pressure was significantly elevated in DBA/2J mice compared to C57BL/6J mice (P<0.01). Both retinal and choroidal BF were significantly decreased in DBA/2J mice in comparison to the age-matched normal C57BL/6J mice across all measurements (P < 0.01). From simulations, the values from the integrated BF analysis method had less partial volume effect, and from in vivo scans, this analysis approach also improved power.ConclusionThe dual eye coil setup allows bilateral eye data acquisition, increasing the amount of data acquired without increasing acquisition times in vivo. The reduced ocular BF found using the improved acquisition and analysis approaches replicated the results of previous studies on DBA/2J mice. The ocular hypertensive stress-induced BF reduction found within these mice may represent changes associated with glaucomatous progression.

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Comparative pan genome analysis of oral Prevotella species implicated in periodontitis

Ibrahim

Subramanian

Anishetty

2017

Funct Integr Genomics

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Prevotella is part of the oral bacterial community implicated in periodontitis. Pan genome analyses of eight oral Prevotella species, P. dentalis, P. enoeca, P. fusca, P. melaninogenica, P. denticola, P. intermedia 17, P. intermedia 17-2 and P. sp. oral taxon 299 are presented in this study. Analysis of the Prevotella pan genome revealed features such as secretion systems, resistance to oxidative stress and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems that enable the bacteria to adapt to the oral environment. We identified the presence of type VI secretion system (T6SS) in P. fusca and P. intermedia strains. For some VgrG and Hcp proteins which were not part of the core T6SS loci, we used gene neighborhood analysis and identified putative effector proteins and putative polyimmunity loci in P. fusca and polymorphic toxin systems in P. intermedia strains. Earlier studies have identified the presence of Por secretion system (PorSS) in P. gingivalis, P. melaninogenica and P. intermedia. We noted the presence of their homologs in six other oral Prevotella studied here. We suggest that in Prevotella, PorSS is used to secrete cysteine proteases such as interpain and C-terminal domain containing proteins with a "Por_secre_tail" domain. We identified subtype I-B CRISPR-Cas system in P. enoeca. Putative CRISPR-Cas system subtypes for 37 oral Prevotella and 30 non-oral Prevotella species were also predicted. Further, we performed a BLASTp search of the Prevotella proteins which are also conserved in the red-complex pathogens, against the human proteome to identify potential broad-spectrum drug targets. In summary, the use of a pan genome approach enabled identification of secretion systems and defense mechanisms in Prevotella that confer adaptation to the oral cavity.

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Imaging rhodopsin degeneration in vivo in a new model of ocular ischemia in living mice

Cited by 4 publications

References 35 publications

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

Improved MRI methods to quantify retinal and choroidal blood flow applied to a model of glaucoma

Comparative pan genome analysis of oral Prevotella species implicated in periodontitis

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