Imaging Techniques for Quantifying Passive Diffusion Across Lipid Bilayer Membranes

Abstract

in the wheat-germ-extract (WGE) cell-free system, showing that the ring can be formed with the c subunit produced with a WGE cell-free system. Then, solid-state NMR analysis was carried out. Two-dimensional 13 C homonuclear correlation with DARR and 2D Ca(iþ1)-Ca(i) correlation experiments were performed under MAS. The assignment was carried out, using fully 13 C, 15 N labeled TF o c and partially 13 C, 15 N labeled TF o c. The assignment strategy and the obtained structural information on the basis of the assignment and magnetization transfer will be discussed.