Imaging the hard/soft tissue interface

Bannerman, Alistair; Paxton, Jennifer Z.; Grover, Liam M.

doi:10.1007/s10529-013-1374-4

Cited by 12 publications

(7 citation statements)

References 90 publications

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“…The fibrin‐contraction approach to forming 3‐D tissues that underpins this organotypic bone system has been created and diversified over the past 8 years to suit a variety of cell phenotypes (Baar, ; Bannerman, Paxton, & Grover, ; Huang, Dennis, Larkin, & Baar, ; Iordachescu et al., ; Iordachescu et al., ; Koburger, Bannerman et al. ; Lebled, Grover, & Paxton, ; Mehrban et al., ; Paxton, Donnelly, Keatch, Baar, & Grover, ; Paxton, Grover et al.…”

Section: Commentarymentioning

confidence: 99%

Organotypic Culture of Bone‐Like Structures Using Composite Ceramic‐Fibrin Scaffolds

Iordachescu

Williams

Hulley

et al. 2019

CP Stem Cell Biology

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We have developed an organotypic culture system that allows the production of bone tissue features on a centimeter scale. A composite, calcium phosphatestrained fibrin gel system is able to organize itself in the presence of osteoblastic cells, creating basic hierarchical units as seen in vivo, and can be modified to produce a range of other tissues that require such directional structuring. Constructs evolve over time into multi-compositional structures containing a high mineral content and terminally differentiated, osteocyte-like cells. These tissues can be cultured over extended durations (exceeding 1 year) and are responsive to a variety of chemical and biological agents. The platform can reduce the number of animals used in experimentation by acting as an intermediate stage in which more personalized research conditions can be generated. We provide a thorough description of the protocol used to successfully culture and modify this system, as well as guidance on compositional characterization. C 2019 by John Wiley & Sons, Inc. Keywords: biomaterials r bone r organotypic culture r osteocytes r selforganization How to cite this article: Iordachescu, A., Williams, R. L., Hulley, P. A., & Grover, L. M. (2019). Organotypic culture of bone-like structures using composite ceramic-fibrin scaffolds.

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Section: Commentarymentioning

confidence: 99%

Organotypic Culture of Bone‐Like Structures Using Composite Ceramic‐Fibrin Scaffolds

Iordachescu

Williams

Hulley

et al. 2019

CP Stem Cell Biology

Self Cite

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“…review by Bannerman et al. ). The most relevant concepts can be explained in any histological section along the inserting tendon fibers.…”

Section: Introductionmentioning

confidence: 99%

Three‐dimensional imaging of the fibrous microstructure of Achilles tendon entheses in Mus musculus

et al. 2018

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The whole-organ, three-dimensional microstructure of murine Achilles tendon entheses was visualized with micro-computed tomography (microCT). Contrast-enhancement was achieved either by staining with phosphotungstic acid (PTA) or by a combination of cell-maceration, demineralization and critical-point drying with low tube voltages and propagation-based phase-contrast (fibrous structure scan). By PTA-staining, X-ray absorption of the enthesial soft tissues became sufficiently high to segment the tendon and measure cross-sectional areas along its course. With the fibrous structure scans, three-dimensional visualizations of the collagen fiber networks of complete entheses were obtained. The characteristic tissues of entheses were identified in the volume data. The tendon proper was marked as a segment manually. The fibers within the tendon were marked by thresholding. Tendon and fiber cross-sectional areas were measured. The measurements were compared between individuals and protocols for contrast-enhancement, using a spatial reference system within the three-dimensional enthesis. The usefulness of the method for investigations of the fibrous structure of collagenous tissues is demonstrated.

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“…Recently, the application of the high‐resolution X‐ray tomography (called also micro‐computed tomography (CT), micro‐CT or μCT) significantly increased. It is a non‐destructive technique that provides 3D images of the internal structure in materials from different life domains . The image analysis offers the possibility to study in detail the size and shape of each individual particle present in granular and cementitious materials.…”

Section: Introductionmentioning

confidence: 99%

Experimental Investigations of Fracture Process in Concrete by Means of X‐ray Micro‐computed Tomography

Skarżyński

Tejchman

2015

Strain

116

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The paper describes investigation results on fracture in notched concrete beams under quasi‐static three‐point bending by the X‐ray micro‐computed tomography. The two‐dimensional (2D) and three‐dimensional image procedures were used. Attention was paid to width, length, height and shape of cracks along beam depth. In addition, the displacements on the surface of concrete beams during the deformation process were measured with the 2D digital image correlation technique in order to detect strain localisation before a discrete crack occurred. The 2D fracture patterns in beams were numerically simulated with the finite‐element method using an isotropic damage constitutive model enhanced by a characteristic length of micro‐structure. Concrete was modelled as a random heterogeneous four‐phase material composed of aggregate, cement matrix, interfacial transitional zones and air voids. The advantages of the X‐ray micro‐computed tomography were outlined.

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Imaging the hard/soft tissue interface

Cited by 12 publications

References 90 publications

Organotypic Culture of Bone‐Like Structures Using Composite Ceramic‐Fibrin Scaffolds

Organotypic Culture of Bone‐Like Structures Using Composite Ceramic‐Fibrin Scaffolds

Three‐dimensional imaging of the fibrous microstructure of Achilles tendon entheses in Mus musculus

Experimental Investigations of Fracture Process in Concrete by Means of X‐ray Micro‐computed Tomography

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