2020
DOI: 10.1002/jbio.202000129
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Imaging the invisible—Bioorthogonal Raman probes for imaging of cells and tissues

Abstract: A revolutionary avenue for vibrational imaging with super‐multiplexing capability can be seen in the recent development of Raman‐active bioortogonal tags or labels. These tags and isotopic labels represent groups of chemically inert and small modifications, which can be introduced to any biomolecule of interest and then supplied to single cells or entire organisms. Recent developments in the field of spontaneous Raman spectroscopy and stimulated Raman spectroscopy in combination with targeted imaging of biomol… Show more

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Cited by 43 publications
(33 citation statements)
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“…1e, Supplementary Table 1), and (ii) a new peak appeared at ~2,160 cm −1 , which lies in the so-called “silent wavenumber region” for which there is no known overlap with Raman signatures of biological origin. This 2,160 cm −1 peak indicates the presence of a triple-bond containing compound (29). Interestingly, many C. reinhardtii cells examined in co-culture with P. protegens had lost their typical eyespot (Fig.…”
Section: Resultsmentioning
confidence: 98%
“…1e, Supplementary Table 1), and (ii) a new peak appeared at ~2,160 cm −1 , which lies in the so-called “silent wavenumber region” for which there is no known overlap with Raman signatures of biological origin. This 2,160 cm −1 peak indicates the presence of a triple-bond containing compound (29). Interestingly, many C. reinhardtii cells examined in co-culture with P. protegens had lost their typical eyespot (Fig.…”
Section: Resultsmentioning
confidence: 98%
“…Metabolic changes of proteins and lipids were also studied in cancer cells during epithelial-mesenchymal transition [142]. Compared with approaches like SILAC or fluorescence, this approach with Raman-SIP could examine the proteome of a cell in a spatially resolved, non-destructive manner on living single cells with only minor modifications [145]. This approach could also be used to study protein degradation using 13 C labelled amino acids [134,135].…”
Section: Raman-sip Monitors Intracellular Metabolic Activitiesmentioning
confidence: 99%
“…A rapidly evolving research area is the application of Raman tags and development of Raman probes. 80 Raman tags can be stable isotopes, such as deuterium or -atoms, or functional groups, such as alkyne and nitrile, which create signals in a typically silent region of biological Raman spectra. A huge benefit is the relatively small size of the tags compared to the often bulky and large fluorescence dyes, which most likely alter the biological activity especially when introduced into cells.…”
Section: Optical Modalities For Medical Diagnosticsmentioning
confidence: 99%