Imaging voltage and brain chemistry with genetically encoded sensors and modulators

Pal, Akash; Tian, Lin

doi:10.1016/j.cbpa.2020.07.006

Cited by 23 publications

(15 citation statements)

References 62 publications

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“…Sun et al, 2020). Improvements in voltage indicators could enable future wide-field voltage imaging to capture macroscopic dynamics at millisecond resolution with cell type specificity and longitudinal monitoring (Knöpfel and Song, 2019;Piatkevich et al, 2019;Pal and Tian, 2020). Furthermore, indicators targeting specific subcellular compartments (e.g., soma, axon) will help further determine the relative contributions of different sources in wide-field signals (Broussard et al, 2018;Y.…”

Section: Expanding Toolkits Of Novel Genetically Encoded Indicators and Miniaturized Imaging Devicesmentioning

confidence: 99%

Characterizing Cortex-Wide Dynamics with Wide-Field Calcium Imaging

Ren

Komiyama

2021

J. Neurosci.

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The brain functions through coordinated activity among distributed regions. Wide-field calcium imaging, combined with improved genetically encoded calcium indicators, allows sufficient signal-to-noise ratio and spatiotemporal resolution to afford a unique opportunity to capture cortex-wide dynamics on a moment-by-moment basis in behaving animals. Recent applications of this approach have been uncovering cortical dynamics at unprecedented scales during various cognitive processes, ranging from relatively simple sensorimotor integration to more complex decision-making tasks. In this review, we will highlight recent scientific advances enabled by wide-field calcium imaging in behaving mice. We then summarize several technical considerations and future opportunities for wide-field imaging to uncover large-scale circuit dynamics.

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Section: Expanding Toolkits Of Novel Genetically Encoded Indicators and Miniaturized Imaging Devicesmentioning

confidence: 99%

Characterizing Cortex-Wide Dynamics with Wide-Field Calcium Imaging

Ren

Komiyama

2021

J. Neurosci.

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“…However, a number of technical hurdles related to adequate signal-to-noise ratios and stable expression in the plasma membrane of live brain tissue currently limits their widespread use in behaving animals. Alternatively, genetically encoded Ca 2+ indicators (GECIs) such as GCaMPs are widely used in vivo to measure intracellular Ca 2+ flux, which is often interpreted as a proxy of cell firing or neurotransmitter release ( Pal and Tian, 2020 ). Similar intracellular signaling sensors have been developed for monitoring second messenger signals, such as kinases or GTPases ( Rost et al, 2017 ).…”

Section: Conclusion Caveats and Future Directionsmentioning

confidence: 99%

Endocannabinoid Modulation of Nucleus Accumbens Microcircuitry and Terminal Dopamine Release

Covey

Yocky

2021

Front. Synaptic Neurosci.

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The nucleus accumbens (NAc) is located in the ventromedial portion of the striatum and is vital to valence-based predictions and motivated action. The neural architecture of the NAc allows for complex interactions between various cell types that filter incoming and outgoing information. Dopamine (DA) input serves a crucial role in modulating NAc function, but the mechanisms that control terminal DA release and its effect on NAc neurons continues to be elucidated. The endocannabinoid (eCB) system has emerged as an important filter of neural circuitry within the NAc that locally shapes terminal DA release through various cell type- and site-specific actions. Here, we will discuss how eCB signaling modulates terminal DA release by shaping the activity patterns of NAc neurons and their afferent inputs. We then discuss recent technological advancements that are capable of dissecting how distinct cell types, their afferent projections, and local neuromodulators influence valence-based actions.

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“…Further virtually knocking-out individual intrinsic and synaptic mechanisms, introducing intrinsic/synaptic plasticity, synaptic scaling, and dendritic remodeling could help delineate their specific roles in the generation of LFPs under physiological and pathological conditions. From these insights, testable hypotheses can be obtained and experimentally verified using the extensive set of tools available for neuroscience research ( Rivnay et al, 2017 ; Mendrela et al, 2018 ; Kim et al, 2020 ; Moreaux et al, 2020 ; Nectow and Nestler, 2020 ; Pal and Tian, 2020 ; Sabatini and Tian, 2020 ).…”

Section: Coda and Future Directionsmentioning

confidence: 99%