Imaging wound healing using optical coherence tomography and multiphoton microscopy in an in vitro skin-equivalent tissue model

Yeh, Alvin T.; Kao, Bunsho; Jung, Woong Gyu; Chen, Zhongping; Nelson, J. Stuart; Tromberg, Bruce J.

doi:10.1117/1.1648646

Cited by 119 publications

(94 citation statements)

References 20 publications

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“…Developments will need to include faster data acquisition and processing for in vivo real-time recording and enhancement of resolution by use of broad-band light sources, OCT-multiphoton microscopy, or polarization-sensitive OCT (18). Inevitably, such developments will need to be finely balanced against constraints placed by the particular in vivo milieu in which OCT is being used and the complex tissue profile.…”

Section: Discussionmentioning

confidence: 99%

Optical Coherence Tomography: Real-time Imaging of Bronchial Airways Microstructure and Detection of Inflammatory/Neoplastic Morphologic Changes

Whiteman

Yang

Pittius³

et al. 2006

Clinical Cancer Research

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Purpose: Current diagnostic imaging modalities for human bronchial airways do not possess sufficient resolution and tissue penetration depth to detect early morphologic changes and to differentiate in real-time neoplastic pathology from nonspecific aberrations. Optical coherence tomography (OCT) possesses the requisite high spatial resolution for reproducible delineation of endobronchial wall profiling. Experimental Design: To establish whether OCT could differentiate between the composite microstructural layers of the human airways and simultaneously determine in situ morphologic changes, using a bench-top OCT system, we obtained cross-sectional images of bronchi from 15 patients undergoing lung resections for cancer. All scanned sections underwent subsequent detailed histologic analysis, allowing direct comparisons to be made. Results: OCT imaging enables characterization of the multilayered microstructural anatomy of the airways, with a maximum penetration depth up to 2 to 3 mm and 10-μm spatial resolution. The epithelium, subepithelial components, and cartilage are individually defined. The acquired OCT images closely match histologically defined patterns in terms of structural profiles. Furthermore, OCT identifies in situ morphologic changes associated with inflammatory infiltrates, squamous metaplasia, and tumor presence. Conclusions: Our results confirm that OCT is a highly feasible optical tool for real-time near-histologic imaging of endobronchial pathology, with potential for lung cancer surveillance applications in diagnosis and treatment.

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Section: Discussionmentioning

confidence: 99%

Optical Coherence Tomography: Real-time Imaging of Bronchial Airways Microstructure and Detection of Inflammatory/Neoplastic Morphologic Changes

Whiteman

Yang

Pittius³

et al. 2006

Clinical Cancer Research

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“…This technique added contrast and enhanced the resolution of OCT due to the strong dependence of SHG on molecular and tissue structures. In the same year, Yeh et al 187 combined OCT with a superluminescent diode centered at 1310 nm and MPM with an ultrafast Ti:sapphire laser (150 fs, 800 nm) in two separate systems and compared it with histopathology to monitor laser thermal injury and subsequent wound healing in organotypic, skin-equivalent tissue models. The in vitro skin-equivalent rat tissue model was composed of dermis with type I collagen and fibroblast cells and epidermis of differentiated keratinocytes.…”

Section: Multimodal Oct and Mpmmentioning

confidence: 99%

Optical coherence tomography today: speed, contrast, and multimodality

Drexler¹,

Li²,

Kumar³

et al. 2014

J. Biomed. Opt

433

264

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Abstract. In the last 25 years, optical coherence tomography (OCT) has advanced to be one of the most innovative and most successful translational optical imaging techniques, achieving substantial economic impact as well as clinical acceptance. This is largely owing to the resolution improvements by a factor of 10 to the submicron regime and to the imaging speed increase by more than half a million times to more than 5 million A-scans per second, with the latter one accomplished by the state-of-the-art swept source laser technologies that are reviewed in this article. In addition, parallelization of OCT detection, such as line-field and full-field OCT, has shortened the acquisition time even further by establishing quasi-akinetic scanning. Besides the technical improvements, several functional and contrast-enhancing OCT applications have been investigated, among which the label-free angiography shows great potential for future studies. Finally, various multimodal imaging modalities with OCT incorporated are reviewed, in that these multimodal implementations can synergistically compensate for the fundamental limitations of OCT when it is used alone. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…In previous literature on skin burns assessment, OCT had mainly been used with a polarization sensitive setup for collagen imaging [36][37][38], but no measurement of the optical attenuation coefficient had been reported. However, other OCT studies found that low attenuation is correlated with low collagen content, in the case of agarose scaffolds [39] and ex vivo ovarian tissue [40], which is consistent with our results.…”

Section: Discussionmentioning

confidence: 99%

Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries

Santos

Latrive

Castro

et al. 2017

Biomed. Opt. Express

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Thousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an in vivo animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy. 3 dorsal areas of 20 anesthetized Wistar rats were burned by water vapor exposure and subsequently treated either by classical surgical debridement, by laser ablation, or left without treatment. Skin burn tissues were noninvasively characterized by OCT images and biopsied for further histopathology analysis, SHG imaging and FTIR spectroscopy at 3, 5, 7 and 14 days after burn. The laser protocol was found as efficient as the classical treatment for promoting the healing process. The study concludes to the validation of femtosecond ultra-short pulses laser treatment for skinburns, with the advantage of minimizing operatory trauma.

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Imaging wound healing using optical coherence tomography and multiphoton microscopy in an in vitro skin-equivalent tissue model

Cited by 119 publications

References 20 publications

Optical Coherence Tomography: Real-time Imaging of Bronchial Airways Microstructure and Detection of Inflammatory/Neoplastic Morphologic Changes

Optical Coherence Tomography: Real-time Imaging of Bronchial Airways Microstructure and Detection of Inflammatory/Neoplastic Morphologic Changes

Optical coherence tomography today: speed, contrast, and multimodality

Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries

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