Imaging β-galactosidase activity using 19F chemical shift imaging of LacZ gene-reporter molecule 2-fluoro-4-nitrophenol-β-d-galactopyranoside

Kodibagkar, Vikram D.; Yu, Jun; Liu, Li; Hetherington, Hoby P.; Mason, Ralph P.

doi:10.1016/j.mri.2006.04.003

Cited by 77 publications

(50 citation statements)

References 17 publications

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“…Examples include enzymes or transporters that can act on synthetic contrast agents, [178] proteins that naturally contain paramagnetic metals, including ferritin, [179] MagA, [180] MntR, [181] tyrosinase, [182] and cytochrome P450, [183] and diamagnetic CEST agents such as lysine rich-protein, [184] human protamine sulfate [185] and proteins that alter water diffusivity in tissue. [186] Comparatively fewer contrast agents have been designed for heteronuclear MRI, all of them based on enzymatic or transporter interactions with 19 F compounds, [187] HP 13 C compounds, [187c, 188] or 31 P substrates. [189] A major challenge of all of these agents is their relatively low molecular sensitivity, typically of the micromolar or higher order, which limits their range of in vivo applications.…”

Section: Mri Contrast Agentsmentioning

confidence: 99%

NMR Hyperpolarization Techniques of Gases

Barskiy

Coffey

Nikolaou

et al. 2016

Chemistry A European J

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Nuclear spin polarization can be significantly increased through the process of hyperpolarization, leading to an increase in the sensitivity of nuclear magnetic resonance (NMR) experiments by 4-8 orders of magnitude. Hyperpolarized gases, unlike liquids and solids, can be more readily separated and purified from the compounds used to mediate the hyperpolarization processes. These pure hyperpolarized gases enabled many novel MRI applications including the visualization of void spaces, imaging of lung function, and remote detection. Additionally, hyperpolarized gases can be dissolved in liquids and can be used as sensitive molecular probes and reporters. This mini-review covers the fundamentals of the preparation of hyperpolarized gases and focuses on selected applications of interest to biomedicine and materials science.

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Section: Mri Contrast Agentsmentioning

confidence: 99%

NMR Hyperpolarization Techniques of Gases

Barskiy

Coffey

Nikolaou

et al. 2016

Chemistry A European J

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“…A different reporter molecule (2-fluoro-4-nitrophenol-b-D-galactopyranoside or OFPNPG), which appears to be less toxic, has been suggested for the assessment by 19 F chemical shift imaging (CSI). 185 There have been attempts to develop an imaging method based on the 1 H NMR signals of myoglobin to measure tissue oxygenation in vivo (reference 186 and references therein). The authors reported that a chemical shift imaging strategy using the deoxymyoglobin signal is feasible for monitoring both global as well as localized tissue oxygen distribution in human gastrocnemius muscle.…”

Section: Genetic Reporters For Mrsmentioning

confidence: 99%

CHAPTER 11. Magnetic Resonance‐Based Cell Imaging Using Contrast Media and Reporter Genes

Velde¹,

Himmelreich²

2013

New Developments in NMR

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“…This allows for unambiguous detection of many exogenously administered 19 F markers exhibiting unique spectral signals. These advantages, along with its gyromagnetic ratio comparable to 1 H, make fluorine a suitable marker for molecular imaging [2], cell tracking [1,3], and other biological and medical applications [4][5][6]. The identification of different 19 F markers can be achieved using chemical shift imaging (CSI) [2,7,8].…”

Section: Introductionmentioning

confidence: 99%