Imbalance Between Interleukin-1β and Interleukin-1 Receptor Antagonist in Epicardial Adipose Tissue Is Associated With Non ST-Segment Elevation Acute Coronary Syndrome

Parisi, Valentina; Petraglia, Laura; Cabaro, Serena; D’Esposito, Vittoria; Bruzzese, Dario; Ferraro, Giusy; Urbani, Andrea; Grieco, Fabrizia; Conte, Maddalena; Caruso, Aurelio; Grimaldi, M.; Bellis, Antonio De; Severino, S; Caldarola, Pasquale; Raia, Maddalena; Scalia, Giulia; Castaldo, Giuseppe; Formisano, Pietro; Leosco, Dario

doi:10.3389/fphys.2020.00042

Cited by 25 publications

(15 citation statements)

References 38 publications

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“…In addition to TNF-α, we have found that adipocytes from IGT SO people released higher levels of IL-1β and IP-10, other key cytokines in obesity and in etiology of T2D 34 . TNFα and IL-1β work together to both initiate and propagate the in ammatory process, also inducing the expression of multiple cytokines, among which IP-10 35,36 . IP-10, also known as CXCL10, has been shown to play a deleterious role in obesity as potential inhibitor of AT angiogenesis 37 .…”

Section: Discussionmentioning

confidence: 99%

Subcutaneous Adipose Tissue Cell-derived Cytokines Are Early Markers of Impaired Glucose Tolerance in Severe Obesity

D’Esposito¹,

Ambrosio²,

Liguoro³

et al. 2021

Preprint

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Background Excessive adiposity provides an inflammatory environment. However, in people with severe obesity, how systemic and local adipose tissue (AT)-derived cytokines contribute to worsening glucose tolerance is not clear. Methods 92 severely obese (SO) individuals undergoing bariatric surgery were enrolled and subjected to detailed clinical phenotyping. Following an Oral Glucose Tolerance Test, participants were included in three groups, based on the presence of normal glucose tolerance (NGT), impaired glucose tolerance (IGT) or Type 2 Diabetes (T2D). Serum and subcutaneous AT (SAT) biopsies were obtained and Mesenchymal Stem Cells (MSCs) were isolated, characterized and differentiated in adipocytes in vitro. TNFA and PPARG mRNA levels were determined by qRT-PCR. Circulating, adipocyte- and MSC-released cytokines, chemokines and growth factors were assessed by multiplex ELISA. Results Serum levels of IL-9, IL-13 and MIP-1β were increased in SO individuals with T2D, as compared with those with either IGT or NGT. At variance, SAT samples obtained from SO individuals with IGT displayed levels of TNFA which were 3-fold higher compared to those with NGT, but not different from those with T2D. Elevated levels of TNFα were also found in differentiated adipocytes, isolated from the SAT specimens of individuals with IGT and T2D, compared to those with NGT. Consistent with the pro-inflammatory milieu, IL-1β and IP-10 secretion was significantly higher in adipocytes from individuals with IGT and T2D. Moreover, increased levels of TNFα, both mRNA and secreted protein, were detected in MSCs obtained from IGT and T2D, compared to NGT SO individuals. Exposure of T2D and IGT–derived MSCs to quercetin reduced TNFα levels and was paralleled by a significant decrease of the secretion of inflammatory cytokines. Conclusion In severe obesity, enhanced SAT-derived inflammatory phenotype is an early step in the progression toward T2D and may be, at least in part, attenuated by quercetin.

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Section: Discussionmentioning

confidence: 99%

Subcutaneous Adipose Tissue Cell-derived Cytokines Are Early Markers of Impaired Glucose Tolerance in Severe Obesity

D’Esposito¹,

Ambrosio²,

Liguoro³

et al. 2021

Preprint

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“…It is responsible for elevation of acute phase reactants such as C-reactive protein, serum amyloid A, fibrinogen, and hepcidin and inhibition of albumin synthesis 18 . IL-1ra has inhibitory roles against pro-inflammatory cytokine activation and T lymphocyte responses 21,22 . It regulates IL-1, TNFα and IFN production 22 , arguing a potential role in constraining a further increase of these cytokines in severe patients.…”

Section: Discussionmentioning

confidence: 99%

Cytokine Signature and COVID-19 Prediction Models in the Two Waves of Pandemics.

Cabaro¹,

D’Esposito²,

Matola

et al. 2021

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In Europe, two waves of infections with SARS-CoV-2 (COVID-19) have been observed to date. Here, we have investigated whether common patterns of cytokines could be detected in individuals with mild and severe forms of COVID-19 in the two pandemic waves, and whether machine learning approach could be useful to identify the best predictors. An increasing trend of multiple cytokines was observed in patients with mild or severe/critical symptoms of COVID-19, compared with healthy volunteers. Linear Discriminant Analysis (LDA) clearly recognized the three groups based on cytokine patterns. Classification and Regression Tree (CART) further indicated that IL-6 discriminated controls and COVID-19 patients, whilst IL-8 defined disease severity. During the second wave of pandemics, a less intense cytokine storm was observed. CART analysis revealed that IL-6 was the most robust predictor of infection and discriminated moderate COVID-19 patients from healthy controls, regardless of epidemic peak curve. Thus, serum cytokine patterns provide non-invasive biomarkers useful for COVID-19 diagnosis and prognosis. Further definition of individual cytokines may allow to envision novel therapeutic options and pave the way to set up innovative diagnostic tools.

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“…IL-1β is a kind of pro-in ammatory cytokines,to a certain extent,production and release of IL-1β can resistance infection,but at the same time,there are correlations with gout,rheumatoid arthritis,chronic kidney disease,atherosclerosis and so on [27][28] .Studies have demonstrated that [29] IL-1β can promote the recruitment of neutrophil in the part of in ammation so that cause gout.In addition,IL-1β can make the person produces strong pain by activation injured receptor which exist in peripheral sensory nerve system sensitively and directly.The processing and secretion of IL-1β not only can through NOD-like receptor signaling pathways,but also can by Toll-like receptor signaling pathways.When MSU crystals are identi ed by Toll-like receptor,TLRs are able to interact with MyD88,make the MyD88 activation,leading to the activation of nuclear factor kappa B (NF-κB) and enter the nucleus to produce IL-1β,cause in ammation eventually through the cascade ampli cation effect [30][31] .…”

Section: Discussionmentioning

confidence: 99%

Associations Between the NLRP3 and IL-1β Gene Polymorphisms and Gout Susceptibility

Chen

et al. 2020

Preprint

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Objective Gout is a common form of inflammatory arthritis which characteristics by joint and surrounding soft tissues pain with sudden onset,fever,redness and swelling,caused by the monosodium urate crystals deposited.Studies have demonstrated that genetic factors occupies a certain role in the pathogenesis of gout.Therefore,in our study we aimed to explore the associations between the NLRP3 and IL-1β gene polymorphisms and gout susceptibility,in order to provide experimental basis for the diagnosis and treatment of gout furtherly.Methods We selected 715 Han individuals as our research object which included 267 male gout patients and 448 male healthy controls.Recorded demographic informations and collected blood samples.Then measured biochemical indicators and extracted the genomic DNA.The genotypes of NLRP3 gene rs7525979,rs3806268 and rs10754558 loci and IL-1β gene rs16944,rs1143623 and rs1143634 loci were detected through polymerase chain reaction(PCR) amplification technique.All data were analysised used Statistical Package for Social Sciences version 23.0(SPSS 23.0) software.Results The distribution of genotype and allele frequency were significant differences in gout patients and controls for rs3806268 of NLRP3 gene and rs1143634 of IL-1β gene(P༜0.05).NLRP3 gene rs3806268 loci AA genotype compared with GG genotype in additive model(OR = 0.58,95%CI:0.41–0.84) and AA genotype compared with GG + GA genotype in recessive model(OR = 0.61, 95%CI:0.44–0.85) and IL-1β gene rs1143634 loci AA + GA genotype compared with GG genotype in dominant model(OR = 0.45, 95%CI:0.24–0.84) could reduced the risk of gout.TC and LDL-C concentrations were significantly differences when gout patients were divided into AA + GA and GG genotype groups (P༜0.05).Conclusion There are some associations between rs3806268 of NLRP3 gene and rs1143634 of IL-1β gene and gout susceptibility.Rs3806268 and rs1143634 loci variants may be the protect factors of gout.

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Imbalance Between Interleukin-1β and Interleukin-1 Receptor Antagonist in Epicardial Adipose Tissue Is Associated With Non ST-Segment Elevation Acute Coronary Syndrome

Cited by 25 publications

References 38 publications

Subcutaneous Adipose Tissue Cell-derived Cytokines Are Early Markers of Impaired Glucose Tolerance in Severe Obesity

Subcutaneous Adipose Tissue Cell-derived Cytokines Are Early Markers of Impaired Glucose Tolerance in Severe Obesity

Cytokine Signature and COVID-19 Prediction Models in the Two Waves of Pandemics.

Associations Between the NLRP3 and IL-1β Gene Polymorphisms and Gout Susceptibility

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