Imidacloprid induced histomorphological changes and expression of TLR-4 and TNFα in lung

Pandit, Arif Ahmad; Choudhary, Shanti; Ramneek,; Singh, Baljit; Sethi, R. S.

doi:10.1016/j.pestbp.2016.02.004

Cited by 31 publications

(28 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have demonstrated that TNF-α activates the expression of endothelial adhesion molecules to facilitate migration of neutrophils into inflamed lungs [26]. Similarly, in our acute lung infection mouse models, we found that wild-type mice infected with PAO1 showed significantly increased TNF-α protein expression as well as increased IL-6 expression.…”

Section: Discussionsupporting

confidence: 80%

Effects of Pseudomonas aeruginosa and Streptococcus mitis mixed infection on TLR4-mediated immune response in acute pneumonia mouse model

Song

Zhang

et al. 2017

BMC Microbiol

View full text Add to dashboard Cite

BackgroundOur previous research on the diversity of microbiota in the endotracheal tubes (ETTs) of neonates in the neonatal intensive care unit found that Pseudomonas aeruginosa (P. aeruginosa) and Streptococcus mitis (S. mitis) were the dominant bacteria on the ETT surface and the existence of S. mitis could promote biofilm formation and pathogenicity of P. aeruginosa. Toll-like receptor 4 (TLR4), which has been widely detected on the surface of airway epithelial cells, is the important component of the innate immune system. Therefore, we hypothesized that the co-existence of these two bacteria might impact the host immune system through TLR4 signaling.Results S. mitis rarely caused inflammation, whereas P. aeruginosa caused the most severe inflammation accompanied by increases in the number of inflammatory cells, interleukin (IL)-6 and tumor necrosis factor (TNF)-α expression, and total cell counts in BALF (p < 0.05). In the PAO1 + S. mitis group, moderate inflammation, reduced IL-6 and TNF-α protein levels, and decreased total cell counts were observed. Additionally, levels of these indicators were decreased lower in TLR4-deficient mice than in wild-type mice (p < 0.05).ConclusionsOur results demonstrated that infection with S. mitis together with P. aeruginosa could alleviate lung inflammation in acute lung infection mouse models possibly via the TLR4 signaling pathway.

show abstract

Section: Discussionsupporting

confidence: 80%

Effects of Pseudomonas aeruginosa and Streptococcus mitis mixed infection on TLR4-mediated immune response in acute pneumonia mouse model

Song

Zhang

et al. 2017

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…Exposure to certain insecticides can increase TNF-α activation in the blood and brain [ 25 ]. A few studies have reported that IMI toxicity induces 8-OHdG and TNF-α activation in animals [ 16 , 26 ]. It is not clear whether 8-OHdG and TNF-α are activated in aquatic animals, particularly fish, because the effects of IMI toxicity in these animals are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Imidacloprid exposure cause the histopathological changes, activation of TNF-α, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpio L.)

2018

View full text Add to dashboard Cite

show abstract

“…The immunohistochemistry was performed as described earlier [20]. Briefly tissue sections were de-paraffinized and rehydrated.…”

Section: Methodsmentioning

confidence: 99%

Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice

2016

View full text Add to dashboard Cite

Aim:Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor 9 (TLR-9) in mice.Materials and Methods:In this study, healthy male Swiss albino mice (n=30) aging 8-10 weeks were used to evaluate TLR-9 expression in lungs of mice following indoxacarb exposure with and without lipopolysaccharide (LPS). Indoxacarb was administered orally dissolved in groundnut oil at 4 and 2 mg/kg/day for 90 days. On day 91, five animals from each group were challenged with LPS/normal saline solution at 80 µg/animal. The lung tissues were processed for real time and immunohistochemical studies.Results:LPS resulted increase in fold change m-RNA expression level of TLR-9 as compare to control, while indoxacarb (4 mg/kg) alone and in combination with LPS resulted 16.21-fold change and 29.4-fold change increase in expression of TLR-9 m-RNA, respectively, as compared to control. Similarly, indoxacarb (2 mg/kg) alone or in combination with LPS also altered TLR-9 expression. Further at protein level control group showed minimal expression of TLR-9 in lungs as compare to other groups, however, LPS group showed intense positive staining in bronchial epithelium as well as in alveolar septal cells. Indoxacarb at both doses individually showed strong immuno-positive reaction as compare to control, however when combined with LPS resulted intense staining in airway epithelium as compare to control.Conclusion:Chronic oral administration of indoxacarb for 90 days (4 and 2 mg/kg) alters expression of TLR-9 at m-RNA and protein level and co-exposure with LPS exhibited synergistic effect.

show abstract

Imidacloprid induced histomorphological changes and expression of TLR-4 and TNFα in lung

Cited by 31 publications

References 34 publications

Effects of Pseudomonas aeruginosa and Streptococcus mitis mixed infection on TLR4-mediated immune response in acute pneumonia mouse model

Effects of Pseudomonas aeruginosa and Streptococcus mitis mixed infection on TLR4-mediated immune response in acute pneumonia mouse model

Imidacloprid exposure cause the histopathological changes, activation of TNF-α, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpio L.)

Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice

Contact Info

Product

Resources

About