Imipramine hydrochloride and desipramine hydrochloride as new reagents for detection of microamounts of blood in urine

Syed, Akheel Ahmed; Silwadi, Mohammed Fawaz; Khatoon, Bibi Ahmadi

doi:10.1016/s0731-7085(01)00665-3

Cited by 6 publications

(2 citation statements)

References 17 publications

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“…Imipramine is known to be light sensitive [31], but aqueous solutions of this drug were found in this study to be stable for several weeks when stored in the dark at 4°C. Solutions of warfarin in a pH 7.4, 0.067 M phosphate buffer are also known to be stable for several days when stored at 4°C [32], but solutions of L-tryptophan were prepared daily due to the limited stability of this reagent under such conditions [33,34].…”

Section: Methodsmentioning

confidence: 75%

Studies of imipramine binding to human serum albumin by high-performance affinity chromatography

Yoo

Smith

Hage

2009

Journal of Chromatography B

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Binding by the drug imipramine to the protein human serum albumin (HSA) was studied by using high-performance affinity chromatography. The association equilibrium constants and number of binding sites for imipramine with HSA were first estimated by utilizing frontal analysis. Imipramine was found to have one major binding site on HSA with an association equilibrium constant of 1.6 × 10 5 M −1 at pH 7.4 and 37°C, as well as a second group of weaker and non-specific binding regions (8-9 mol/mol HSA) with an average association equilibrium constant of 1.5 × 10 3 M −1 . Competition studies based on zonal elution were performed to identify the location of the major binding site for imipramine on HSA. Imipramine was found to have direct competition with L-tryptophan, which indicated that imipramine was interacting with Sudlow site II, or the indole-benzodiazepine site of HSA. No competition or allosteric effects were noted between imipramine and warfarin, a probe for Sudlow site I or the warfarin-azapropazone site of HSA. The association equilibrium constant found for imipramine at its site of competition with L-tryptophan also agreed with the value that was obtained for the major binding site of imipramine in the frontal analysis studies. These results confirmed that Sudlow site II was the location of the major binding site for imipramine on HSA. These results gave good agreement with previous observations made in the literature and should provide a more detailed description of how imipramine is transported in blood and of how it may interact with other drugs in the body.

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Section: Methodsmentioning

confidence: 75%

Studies of imipramine binding to human serum albumin by high-performance affinity chromatography

Yoo

Smith

Hage

2009

Journal of Chromatography B

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“…Tricyclic antidepressants undergo irreversible oxidation in nitric acid at room temperature [21] and the resulting blue dye can be used to determine the drugs. The sole structural requirement for this reaction is the presence of a nitrogen atom in the central ring, which seems to play a key role in the process.…”

Section: Selection Of the Determination Methodsmentioning

confidence: 99%

Fast urinary screening for imipramine and desipramine using on-line solid-phase extraction and selective derivatization

Cruz-Vera

Lucena

Cárdenas

et al. 2007

Journal of Chromatography B

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Electrochemical Oxidation of Desipramine Drug in the Presence of 4,6‐Dimethylpyrimidine‐2‐thiol Nucleophile in Aqueous Acidic Medium

2015

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1IntroductionTr icyclic antidepressant drugs (TCAs)s uch desipramine (3-(10,11-Dihydro-5H-dibenzo[b,f]azepin-5-yl)-Nmethylpropan-1-amine), are active ingredients of psychiatric drugs widely used in the treatment of depressive disorders [1].T hese tricyclic antidepressants (TCAs)a re used primarily in the clinicalt reatment of mood disorders such as major depressive disorder, dysthymia, and bipolar disorder, especially of the treatment-resistant variants.H owever,t heir overdosei sf atal to the centraln ervous system and may result in drowsiness,c onvulsions,r espiratory disorders,o phthalmoplegia and finally coma [2].M oreover, this drug has also proved to be effective for the treatment of otheri llnesses in addition to depression [3].D esipramine is ad ibenzazepine compound belongs to an important family of antidepressant drugs that inhibits the reuptake of norepinephrine.D esipramine is an active metabolite of imipramine,h avingw ide applicability in pharmacological fields as antinociceptive [4] antithyroid [5],f or detection blood in urine [ 6],i ndeed all these activities are increased upon complexation with electron acceptors.In recent years considerable effort has been expended in elucidating the mechanisms of electrolytic oxidation of heterocyclic nitrogen and amine compounds as well as other nitrogen containing species.O ne of the prominent characteristics of amines is oxidativec oupling to form av ariety of dimerized derivatives of the original parent compounds [7].C ertain heterocyclic nitrogen compounds such as dibenzazepines which may be considered as being derivedf rom desipraminea lso undergo as imilar coupling processo no xidation [8][9][10].E lectrooxidation of compounds containing amineg roups were reported by many workers at different conditionss ucha sa queous and non aqueouss olvents or at various pH values [7].A mong the electrochemical methods,c yclic voltammetry presents very versatile tool fort he kinetic and mechanistic studies of electron transferr eactions and electrochemicals ynthesis of organic and inorganic compounds.F urthermore, cyclic voltammetry is knowna sapowerfult echnique for the investigation of electrochemicalr eactions that are coupled with chemicalr eactions [11][12][13][14][15][16].Thee lectrochemical behavioro ft he desipramine was consistentw ith an ECE mechanism [17].C onsidering these facts,t he aim of this work is to study the electrochemical oxidation behavioro fd esipramine in the presenceo fD MPT at ag lassy carbone lectrode using cyclic voltammetry and controlled-potential electrolysis methods in an aqueous acidic medium. This work also represents an electrochemical process for the synthesis of anew derivative of dibenzazepine via electrochemical oxidation of desipramine( 1) in the presenceo fD MPT under green conditions.Abstract:E lectrochemical oxidation of desipramine (DES) has been studied in the presence of 4,6-dimethylpyrimidine-2-thiol (DMPT) as nucleophile in acidic mediumb ym eans of cyclic voltammetry,c ontrolled-potential electrolysis and spectroscopi...

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Imipramine hydrochloride and desipramine hydrochloride as new reagents for detection of microamounts of blood in urine

Cited by 6 publications

References 17 publications

Studies of imipramine binding to human serum albumin by high-performance affinity chromatography

Studies of imipramine binding to human serum albumin by high-performance affinity chromatography

Fast urinary screening for imipramine and desipramine using on-line solid-phase extraction and selective derivatization

Electrochemical Oxidation of Desipramine Drug in the Presence of 4,6‐Dimethylpyrimidine‐2‐thiol Nucleophile in Aqueous Acidic Medium

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