Imiquimod-Induced Psoriasis Mice Model: A Promising Tool for Psoriasis Research?

Badanthadka, Murali; D’Souza, Lidwin

doi:10.5958/0974-360x.2020.00621.6

Cited by 8 publications

(2 citation statements)

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“…Several immunological pathways and cytokines involved in the disease's genesis and progression were discovered using this model. 27 Despite its advantages, this model still suffers from significant drawbacks, one of which includes its dependency on the animal strain. Flutter and Nestle summarized the strain-dependent effect of various mice, including Balb/c mice 5 that developed disease more rapidly and causes more flaky and inflamed skin than in C57bl/6 mice.…”

Section: Discussionmentioning

confidence: 99%

Differential Psoriatic Effect of Imiquimod on Balb/c and Swiss Mice

Salwa

Badanthadka

D’Souza³

2021

Journal of Health and Allied Sciences NU

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Introduction The influence of animal strain on psoriasis model development by imiquimod (IMQ) has been studied in Balb/c and Swiss mice. Materials and Methods Female mice of either strain were challenged with 5% IMQ (62.5 mg on back skin, 10 mg on right ear). They were observed for the severity of the disease using Psoriasis area severity index (PASI), splenomegaly, and histopathological alterations. To validate the model, well-established antipsoriatic drug clobetasol (0.05%, 120 mg on the back skin, 10 mg on the right ear) was used. Additionally, to study the strain-dependent response to IMQ associated with oxidative stress, various antioxidant factors like superoxide dismutase (SOD), catalase (CT), and glutathione (GSH) were measured. Antioxidant natural product curcumin (1%, 150 mg on back skin, 12.5 mg on right ear) was used to evaluate the alleviation of oxidative stress on distinct mice strain. Results PASI score, body weight, and histopathology indicated the development of disease in both the strains, severity, and stability of which was dependent on the particular strain. Splenomegaly suggested the systemic effect, which was comparable in both the stains. IMQ and its involvement in redox status were confirmed by an alteration in the activity of SOD, CT, and levels of GSH. Conclusion This study demonstrated that, in the IMQ-induced psoriasis model, the genetic background has some impact on the disease severity, stability, and redox imbalance.

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Section: Discussionmentioning

confidence: 99%

Differential Psoriatic Effect of Imiquimod on Balb/c and Swiss Mice

Salwa

Badanthadka

D’Souza³

2021

Journal of Health and Allied Sciences NU

Self Cite

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“…This model is frequently employed as an initial step in research investigations. 19,20 These alternative animal models serve as valuable resources for investigating various facets of psoriasis, whether it involves exploring the immune response, delivering into genetic factors, or dissecting specific cytokine pathways. Researchers typically select the model that best aligns with their research objectives and the particular dimension of psoriasis they intend to scrutinize.…”

Section: Imiquimod (Imq) Application Modelmentioning

confidence: 99%

RNA-Based Antipsoriatic Gene Therapy: An Updated Review Focusing on Evidence from Animal Models

Lin,

Hung,

Aljuffali

et al. 2024

DDDT

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Psoriasis presents as a complex genetic skin disorder, characterized by the interaction between infiltrated immune cells and keratinocytes. Substantial progress has been made in understanding the molecular mechanisms of both coding and non-coding genes, which has positively impacted clinical treatment approaches. Despite extensive research into the genetic aspects of psoriasis pathogenesis, fully grasping its epigenetic component remains a challenging endeavor. In response to the pressing demand for innovative treatments to alleviate inflammatory skin disorders, various novel strategies are under consideration. These include gene therapy employing antisense nucleotides, silencing RNA complexes, stem cell therapy, and antibody-based therapy. There is a pressing requirement for a psoriasis-like animal model that replicates human psoriasis to facilitate early preclinical evaluations of these novel treatments. The authors conduct a comprehensive review of various gene therapy in different psoriasis-like animal models utilized in psoriasis research. The animals included in the list underwent skin treatments such as imiquimod application, as well as genetic and biologic injections, and the results of these interventions are detailed. Animal models play a crucial role in translating drug discoveries from the laboratory to clinical practice, and these models aid in improving the reproducibility and clinical applicability of preclinical data. Numerous animal models with characteristics similar to those of human psoriasis have proven to be useful in understanding the development of psoriasis. In this review, the article focuses on RNA-based gene therapy exploration in different types of psoriasis-like animal models to improve the treatment of psoriasis.

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