Immature platelet fraction for prediction of platelet engraftment after allogeneic stem cell transplantation

Takami, Akiyoshi; Shibayama, Masami; Orito, Matsuo; Omote, Mika; Okumura, Hirokazu; Yamashita, Takeshi; Shimadoi, Shigeru; Yoshida, Tomotaka; Nakao, Shinji; Asakura, Hitoshi

doi:10.1038/sj.bmt.1705623

Cited by 61 publications

(83 citation statements)

References 20 publications

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“…The previously reported reference interval using the Sysmex XE-2100 for healthy adults is between 0.4% and 10% [1,9,[22][23][24][25][26][27]. The limitations of these reported reference intervals are: 1) small control groups, including less than 150 individuals, 2) incorrect application or lack of application of the CLSI guideline for determining reference intervals, and 3) exclusion of pediatric individuals in the control group.…”

Section: Discussionmentioning

confidence: 92%

Immature Platelet Fraction: Establishment of a Reference Interval and Diagnostic Measure for Thrombocytopenia

et al. 2010

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451Background: Immature platelet fraction (IPF, %) is a measure of reticulated platelets (RPs), which represents the state of thrombopoiesis. The IPF is obtained from an automated hematology analyzer as one of the platelet parameters. This study was performed to establish reference intervals of IPF and its cut-off values for the differential diagnosis of thrombocytopenia.Methods: Blood samples from 2,039 healthy individuals (1,161 males, 878 females) were obtained to establish reference intervals. The patient group included patients with idiopathic thrombocytopenic purpura (ITP) (N=150) and aplastic anemia (AA) (N=51) with platelet counts of less than 100×10 9 /L. We evaluated the reliability of the IPF measurements, the reference intervals, and cut-off value for the diagnosis of ITP.Results: The reference intervals of IPF were 0.5-3.2% in males and 0.4-3.0% in females (95% confidence interval). The median IPF% of ITP and AA were 7.7% (range, 1.0-33.8%) and 3.5% (range, 0.6-12.9%), respectively. Statistical analysis revealed a significant difference between the IPF% of ITP and AA (P<0.0001). The cut-off value of IPF for differentiating ITP from AA was 7.3% with a sensitivity and specificity of 54.0% and 92.2%, respectively.Conclusions: A rapid and inexpensive automated measurement of IPF can be integrated as a standard parameter to evaluate the thrombopoietic state of the bone marrow. This study determined the reference intervals of IPF from a large population of healthy individuals, including children. Further studies are needed to establish the clinical utility of IPF. (Korean J Lab Med 2010;30:451-9)

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Section: Discussionmentioning

confidence: 92%

Immature Platelet Fraction: Establishment of a Reference Interval and Diagnostic Measure for Thrombocytopenia

et al. 2010

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“…It is most useful for identifying thrombocytopenia due to peripheral platelet destruction, in which the reticulated platelet count is increased [16]. For hematopoietic stem cell transplantation, the increase in the IPF after transplantation precedes the increase in the total platelet count [17]. The possibility of predicting platelet regeneration a few days after an increase in the reticulated platelet count enables prevention of unnecessary prophylactic platelet transfusion in patients undergoing transplantation or receiving chemotherapy.…”

Section: Discussionmentioning

confidence: 98%

Hematopoietic progenitor cell count, but not immature platelet fraction value, predicts successful harvest of autologous peripheral blood stem cells

Mitani

Yujiri

Tanaka

et al. 2010

J of Clinical Apheresis

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Mobilized stem cells in the peripheral blood (PB) must be efficiently harvested at the appropriate time before autologous PB stem cell (PBSC) transplantation. Enumeration of CD34+ cells in the PB before apheresis predicts the number of PBSCs that can be collected, but the cytometric techniques used are complex and expensive. Therefore, it is necessary to identify an alternative to the CD34+ cell count in PBSC harvest-time monitoring. Fully automated flow cytometry using blood cell counters now allows reliable quantification of immature myeloid cells in the PB, referred to as hematopoietic progenitor cells (HPC), and reticulated platelets, expressed as the immature platelet fraction (IPF). Immature or reticulated platelets are thought to correlate with thrombopoietic activity of the marrow. Following a chemotherapy nadir, the recovery of white blood cell and platelet counts has been used to determine the right time for apheresis. Therefore, we examined whether the HPC count and IPF value could be used to predict PBSC mobilization in 20 patients with hematological malignancies. The HPC count was found to be correlated with the CD34+ cell count (r = 0.84, P < 0.01), whereas the IPF value was not (r = 0.37, P = 0.44). Therefore, the HPC count, but not the IPF value, is a possible predictor of the timing of autologous stem cell transplantation.

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“…It has been previously reported that an IPF % increase precedes an increase in platelet count in several thrombocytopenic conditions e.g. after bone marrow transplant, and chemotherapy-induced thrombocytopenia [11,36,37]. Recently, Yamaoka et al [38] demonstrated that the IPF % peak value was correlated with the period from the day of the peak IPF % to the day of platelet recovery in adult patients undergoing chemotherapy, and hematopoietic stem cell transplantation.…”

Section: Discussionmentioning

confidence: 94%

Immature platelet fraction values predict recovery of platelet counts following liver transplantation

Mao

Lou

et al. 2015

Clinics and Research in Hepatology and Gastroenterology

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Immature platelet fraction for prediction of platelet engraftment after allogeneic stem cell transplantation

Cited by 61 publications

References 20 publications

Immature Platelet Fraction: Establishment of a Reference Interval and Diagnostic Measure for Thrombocytopenia

Immature Platelet Fraction: Establishment of a Reference Interval and Diagnostic Measure for Thrombocytopenia

Hematopoietic progenitor cell count, but not immature platelet fraction value, predicts successful harvest of autologous peripheral blood stem cells

Immature platelet fraction values predict recovery of platelet counts following liver transplantation

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