Immediate and carryover effects of Gram-negative and Gram-positive toxin-induced mastitis on follicular function in dairy cows

Lavon, Y.; Leitner, Gabriel; Moallem, U.; Klipper, Eyal; Voet, Hillary; Jacoby, Shamay; Glick, G.; Meidan, Rina; Wolfenson, D.

doi:10.1016/j.theriogenology.2011.05.001

Cited by 54 publications

(59 citation statements)

References 41 publications

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“…Oocyte developmental competence is acquired in a progressive manner throughout follicular development. The findings of the current study suggest that mastitis not only impairs follicular function (Lavon et al 2011b) but can also affect the follicle-enclosed oocyte. Mastitis induced by GK affected both nuclear and cytoplasmic maturation.…”

Section: Discussionmentioning

confidence: 50%

“…Because in the current study oocytes were matured in FF aspirated from mastitic cows, the deleterious effect on oocyte developmental competence should be considered a direct effect on the preovulatory enclosed oocyte that is carried over to the blastocyst stage. Such alterations might explain, in part, the reduced fertility in mastitic cows (Lavon et al 2011b). An epidemiological study (Lavon et al 2011b) showed a long-term effect of E. coli-induced mastitis on conception rate.…”

Section: Discussionmentioning

confidence: 99%

“…Mastitis was induced as described previously by Lavon et al (2011b). The experiment was approved by the local ethics committee of the Hebrew University.…”

Section: Induction Of Mastitismentioning

confidence: 99%

“…Estradiol concentrations in the FF were determined by solidphase RIA kit (Diagnostic Products Corp., Los Angeles, CA, USA) as described previously (Lavon et al 2011b). The assay sensitivity was 8 pg/ml, and the intra-assay coefficient of variation was 3%.…”

Section: Hormone Concentrationsmentioning

confidence: 99%

“…The assay sensitivity was 8 pg/ml, and the intra-assay coefficient of variation was 3%. Progesterone concentration in the FF was analyzed with the solidphase RIA kit against a standard curve prepared from ovariectomized cow plasma (Lavon et al 2011b). The minimum detected amount was 0.2 ng/ml and the intra-and inter-assay coefficients of variation were 8.6 and 9.9% respectively.…”

Section: Hormone Concentrationsmentioning

confidence: 99%

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Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

Asaf

Leitner²,

Furman³

et al. 2014

Reproduction

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Mastitis is associated with decreased fertility in dairy cows. In the current study, we created an experimental model to simulate shortterm mastitis by a single intramammary administration of Gram-negative endotoxin of Escherichia coli origin (GK), or Gram-positive toxin of Staphylococcus aureus origin (GC), to examine the effect of mastitis on oocyte developmental competence. Healthy Holstein cows were synchronized, and follicular fluid (FF) of cows treated with GC or GK and of uninfected cows (controls) was aspirated from the preovulatory follicles by transvaginal ultrasound procedure. The aspirated FF was used as maturation medium for in vitro embryo production. The distribution of matured oocytes into different cortical granule classes and meiotic stages was affected by GK administration (P!0.05) but not by GC administration. The proportion of oocytes that cleaved to two-and four-cell stage embryos (44 h postfertilization) was lower in both GC and GK groups than in controls (P!0.05). Blastocyst formation rate (7-8 days postfertilization) was lower in the GK group (P!0.05) and numerically lower in the GC group compared with their uninfected counterparts. The total cell number in blastocysts did not differ among groups; however, the apoptotic index was higher in the GC group (P!0.05), but not in the GK group, relative to controls. Examining mRNA relative abundance in oocytes and early embryos revealed mastitis-induced alterations in PTGS2 (COX2), POU5F1, and HSF1 but not in SLC2A1 (GLUT1) or GDF9. Results indicate a differential disruptive effect of mastitis induced by GK and GC on oocyte developmental competence in association with alterations in maternal gene expression.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

“…Mastitis was induced as described previously by Lavon et al (2011b). The experiment was approved by the local ethics committee of the Hebrew University.…”

Section: Induction Of Mastitismentioning

confidence: 99%

Section: Hormone Concentrationsmentioning

confidence: 99%

Section: Hormone Concentrationsmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

Asaf

Leitner²,

Furman³

et al. 2014

Reproduction

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Stress‐induced alterations in oocyte transcripts are further expressed in the developing blastocyst

Roth

2018

Molecular Reproduction Devel

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The oocyte achieves its developmental competence through the lengthy process of folliculogenesis. It can therefore potentially be exposed to various stressors while enclosed in the follicle. Oocyte maturation relies mainly on maternal sources. These include nuclear, cytoplasmic, and molecular maturation, which involve DNA and RNA organization. Maternal transcripts are dominant through the first embryonic cleavages, up until embryonic genome activation. Thus, it is suggested that any perturbations during oocyte storage, in particular of the maternal transcripts, might lead to genetic and/or epigenetic changes, which might be further expressed in the developing embryo. The review discusses the effects of three representative stressors-environmental heat stress, endocrine-disrupting compounds (phthalates), and inflammatory stress (mastitis)-shown to be involved in reduced fertility. The review highlights the carryover response from the oocyte to the developing embryo; it includes intracellular and molecular disruptive mechanisms with an emphasis on maternal transcripts. The review provides insights into the oocyte's cellular and molecular responses with an emphasis on the effects of various stressors on the maternal (nuclear and mitochondrial) transcripts and the association with embryonic development. A comparison between stressors might clarify, at least in part, a few open questions. For instance, (a) whether stress-induced alterations share the same mechanism and if so (b) whether this mechanism involves alterations of maternal transcripts; (c) whether stress-induced alterations in the maternal transcript are further expressed at the developing blastocyst stage, that is, after embryonic genome activation.

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Relationships between follicular fluid steroid concentrations and uterine infections in ovarian cystic cows

et al. 2016

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Immediate and carryover effects of Gram-negative and Gram-positive toxin-induced mastitis on follicular function in dairy cows

Cited by 54 publications

References 41 publications

Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

Stress‐induced alterations in oocyte transcripts are further expressed in the developing blastocyst

Relationships between follicular fluid steroid concentrations and uterine infections in ovarian cystic cows

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