PURPOSE: To report the magnitude of changes in heart rate, muscle temperature, blood lactate concentration, blood pressure, and fatigue perception after treadmill jogging and running.METHODS: Eleven healthy male adults (174±7 cm, 72±10 kg) visited the laboratory twice. After a 10-minute rest each visit, subjects performed a treadmill jog (maintaining a speed of 9 km/h at a 1% incline) or a treadmill run (initially 5 km/h at a 1% incline, belt speed, and incline increased by 1 km/h and 0.5% every minute; average speed of 9 km/h). Heart rate and muscle (gastrocnemius) temperature were recorded before, during, and after the treadmill jog/run. Blood lactate concentration, blood pressure, and fatigue perception were assessed before and after. The effect of the condition over time was analysed using parametric or nonparametric tests (p≤.05) with Cohen’s d effect sizes.RESULTS: There was a condition effect over time in heart rate (F1,30=22.16, p<.0001), blood lactate concentration (χ2=34.88, df=3, p<.0001), systolic blood pressure (F1,30=4.18, p=.05), and fatigue perception (F1,30=10.24, p=.003). Specifically, subjects who ran showed a higher heart rate (187 vs. 158 bpm, p<.0001, d=2.11), blood lactate concentration (11.4 vs. 3.8 mmol/L, p<.0001, d=3.39), systolic blood pressure (164 vs. 147 mmHg, p=.006, d=1.34), and fatigue perception (2.7 vs. 5.0 cm, p=.0005, d=1.46) than those who jogged. There was a time effect (F1,30=94.84, p<.0001) on muscle temperature, with a 1.5°C increase in muscle temperature after jogging/running (p<.0001, d=1.88).CONCLUSIONS: While treadmill jogging and running induced a similar temperature increase in the gastrocnemius, running resulted in higher heart rate, blood lactate concentration, systolic blood pressure, and fatigue perception. These results can be used as a basis for planning warm-up protocols.