Immediately implantable extracellular matrix-enriched osteoinductive hydrogel-laden 3D-printed scaffold for promoting vascularized bone regeneration in vivo

Lee, Jae Seo; Nah, Haram; Lee, Dong-Hyun; An, Sang Hyun; Ko, Wan‐Kyu; Lee, Sang Jin; Lee, Seung Yeon; Park, Kyung Min; Lee, Jung Bok; Yi, Hyeong-joong; Kwon, Il Keun; Choi, Kyu-Sun; Heo, Dong Nyoung

doi:10.1016/j.matdes.2022.110801

Cited by 10 publications

(1 citation statement)

References 47 publications

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“…Soft natural materials that have been used to create printable bone tissue include alginate, collagen, dextran, gelatin, gellan gum, fibrinogen, and hyaluronic acid, as shown in Table 2. Although prior studies have mostly restricted the use of hydrogels as fillers inside rigid scaffolds [116][117][118], the development of composite hydrogels exhibiting enhanced printability and mechanical characteristics has facilitated the use of 3D-printed hydrogels as standalone bone scaffolds [119]. To mimic the mechanical properties of natural bone, harder reinforcing materials may be added to the hydrogel bioink.…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

Current Biomedical Applications of 3D-Printed Hydrogels

Barcena,

Dhal,

Patel

et al. 2023

Gels

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Three-dimensional (3D) printing, also known as additive manufacturing, has revolutionized the production of physical 3D objects by transforming computer-aided design models into layered structures, eliminating the need for traditional molding or machining techniques. In recent years, hydrogels have emerged as an ideal 3D printing feedstock material for the fabrication of hydrated constructs that replicate the extracellular matrix found in endogenous tissues. Hydrogels have seen significant advancements since their first use as contact lenses in the biomedical field. These advancements have led to the development of complex 3D-printed structures that include a wide variety of organic and inorganic materials, cells, and bioactive substances. The most commonly used 3D printing techniques to fabricate hydrogel scaffolds are material extrusion, material jetting, and vat photopolymerization, but novel methods that can enhance the resolution and structural complexity of printed constructs have also emerged. The biomedical applications of hydrogels can be broadly classified into four categories—tissue engineering and regenerative medicine, 3D cell culture and disease modeling, drug screening and toxicity testing, and novel devices and drug delivery systems. Despite the recent advancements in their biomedical applications, a number of challenges still need to be addressed to maximize the use of hydrogels for 3D printing. These challenges include improving resolution and structural complexity, optimizing cell viability and function, improving cost efficiency and accessibility, and addressing ethical and regulatory concerns for clinical translation.

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Section: Bone Tissue Engineeringmentioning

confidence: 99%

Current Biomedical Applications of 3D-Printed Hydrogels

Barcena,

Dhal,

Patel

et al. 2023

Gels

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Integrated and Bifunctional Bilayer 3D Printing Scaffold for Osteochondral Defect Repair

Zhang

Nie

et al. 2023

Adv Funct Materials

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Bioinspired scaffolds with two distinct regions resembling stratified anatomical architecture provide potential strategies for osteochondral defect repair and are studied in preclinical animals. However, delamination of the two layers often causes tissue disjunction between the regenerated cartilage and subchondral bone, leading to few commercially available clinical applications. This study develops an integrated poly(ε-caprolactone) (PCL)-based scaffold for repairing osteochondral defects. An extracellular matrix (ECM)incorporated 3D printing composite scaffold (ECM/PCL) coated with ECM hydrogel (E-co-E/PCL) is fabricated as the upper layer, and magnesium oxide nanoparticles coated with polydopamine (MgO@PDA)-incorporated composite scaffold (MD/PCL) is fabricated using 3D printing as the bottom layer. The physicochemical and mechanical properties of the bilayer scaffold meet the requirements in designing and fabricating the osteochondral scaffold, especially a strong interface possessed between the two layers. By in vitro study, the integrated scaffold stimulates proliferation, chondrogenic differentiation, and osteogenic differentiation of human bone mesenchymal stem cells. Moreover, the integrated bilayer scaffold exhibits well repair ability to facilitate simultaneous regeneration of cartilage and subchondral bone after implanting into the osteochondral defect in rats. In addition, cartilage "tidemarks" completely regenerated after 12 weeks of implantation of the bilayer scaffold, which indicates no tissue disjunctions formed between the regenerated cartilage and subchondral bone.

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Three-dimensional printing of biomaterials for bone tissue engineering: a review

El‐Fiqi

2023

Front. Mater. Sci.

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Immediately implantable extracellular matrix-enriched osteoinductive hydrogel-laden 3D-printed scaffold for promoting vascularized bone regeneration in vivo

Cited by 10 publications

References 47 publications

Current Biomedical Applications of 3D-Printed Hydrogels

Current Biomedical Applications of 3D-Printed Hydrogels

Integrated and Bifunctional Bilayer 3D Printing Scaffold for Osteochondral Defect Repair

Three-dimensional printing of biomaterials for bone tissue engineering: a review

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