Immense Essence of Excellence: Marine Microbial Bioactive Compounds

Bhatnagar, Ira; Kim, Se‐Kwon

doi:10.3390/md8102673

Cited by 336 publications

(225 citation statements)

References 117 publications

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“…Marine bacteria have been attracting more attention as a resource for new bioactive compounds recently, as the soil-derived compounds have yielded high numbers of the previously described metabolites (Toledo et al 2006;Bhatnagar and Kim 2010;Trincone 2011). The present study was conducted to optimize a marine bacterium chitinase production and examine its properties.…”

Section: Resultsmentioning

confidence: 99%

A novel halo-alkali-tolerant and thermo-tolerant chitinase from Pseudoalteromonas sp. DC14 isolated from the Caspian Sea

Makhdoumi

Dehghani-Joybari

Mashreghi

et al. 2015

Int. J. Environ. Sci. Technol.

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A novel halo-alkali and thermo-tolerant chitinase was obtained from an isolated strain found in the Caspian Sea. The effects of media composition and various fermentation conditions for the optimization of chitinase production were studied one factor at a time and by response surface methodology. The novel strain, which is designated as strain DC14 and phylogenetically related to the genus Pseudoalteromonas, produced chitinase after 72 h under the following optimal conditions: glucose 1 % (w/v), ammonium sulphate 0.2 % (w/v), chitin 1.07 % (w/ v), pH 8, NaCl 10 % (w/v), inoculums size 2.5 % (v/v), temperature 30°C, CaCl 2 3 mM and MgCl 2 4 mM. Using the statistical optimization method, chitinase production was found to increase from 2.30 to 21.90 U/dl. The enzyme showed maximum activity at 40°C, pH 9 and 10 % NaCl. It was stable in a wide range of temperature from 15 to 65°C, pH from pH 7 to 11 and NaCl concentration from 0 to 15 % (w/v). The molecular weight of the enzyme was estimated by SDS-PAGE to be about 65 kDa. With regard to the halo-alkali and thermo-stable properties of this enzyme, it has potential industrial activity.

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Section: Resultsmentioning

confidence: 99%

A novel halo-alkali-tolerant and thermo-tolerant chitinase from Pseudoalteromonas sp. DC14 isolated from the Caspian Sea

Makhdoumi

Dehghani-Joybari

Mashreghi

et al. 2015

Int. J. Environ. Sci. Technol.

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“…The kingdom of fungi is one of the largest groups of organisms found widely distributed in nature. Marine fungi have the capability to synthesise novel molecules as they exist in special ecological niches (Bhatnagar and Kim 2010; Raj and Vignesh 2011). …”

Section: Introductionmentioning

confidence: 99%

Antiangiogenic, wound healing and antioxidant activity ofCladosporium cladosporioides(Endophytic Fungus) isolated from seaweed (Sargassum wightii)

et al. 2016

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Endophytic fungi from marine seaweeds are the less studied group of organisms with vast medical applications. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the seaweed Sargassum wightii. The morphological characters and the rDNA internal transcribed spacer sequence analysis (BLAST search in Gen Bank database) was used for the identification of endophytic fungus. The antioxidant potential of the ethyl acetate extract of endophytic fungus was assessed by, 1,1-diphenyl-2-picryl-hydrazyl radical scavenging method. The fungal extract was also analysed for reducing power, total phenolic and flavonoid content. Antiangiogenic activity of the fungal extract was studied in vitro by inhibition of wound healing scratch assay and in vivo by Chick chorioallantoic membrane assay. The endophytic fungus was identified as Cladosporium cladosporioides (Gen Bank ID – KT384175). The ethyl acetate extract of C. cladosporioides showed a significant antioxidant and angiosuppressive activity. The ESI-LC-MS analysis of the extract revealed the presence of wide range of secondary metabolites. Results suggest that C. cladosporioides extract could be exploited as a potential source for angiogenic modulators.

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“…If the three-dimensional structures could not be downloaded, they were created using MarvinSketch with the minimization parameters of the MMFF94 force field and a strict optimization limit. Various marine fungi bioactive compounds, whether known specifically as anticancer or not, were included in the marine database, resulting in 268 structures that were downloaded from PubChem and ChemSpider or created using MarvinSketch [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33].…”

Section: Preparation Of the Marine Databasementioning

confidence: 99%

Database Compilation and Virtual Screening of Secondary Metabolites Derived From Marine Fungi as Epidermal Growth Factor Receptor Tyrosine Abstract Kinase Inhibitors

Watty

Syahdi

Yanuar

2017

Asian J Pharm Clin Res

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Objective: Epidermal growth factor receptor (EGFR), a transmembrane protein with cytoplasmic kinase activity, transduces growth factor signaling from the extracellular space to the cell. EGFR downstream signaling increases proliferation and reduces apoptosis. Agents that are targeted at intracellular tyrosine kinase are tyrosine kinase inhibitor small molecules, which have a mechanism of action that affects adenosine triphosphate binding to the receptor. The exploration of bioactive compounds from marine materials, including marine fungi, has become a major interest lately for anticancer treatment. Methods:In this research, a database was created and in silico screening was conducted using AutoDock and Vina to obtain potential marine fungi bioactive compounds as EGFR-tyrosine kinase (EGFR-TK) inhibitors, which act as antiproliferative agents on tumor cell growth. Results:This research has concluded that the three marine fungi compounds with the lowest binding free energy, FU0015, FU0051, and FU0202, have great potential as inhibitors of EGFR-TK.Keywords: Marine fungi compounds, Epidermal growth factor receptor-tyrosine kinase, Virtual screening, AutoDock, Vina, Antiproliferative. INTRODUCTIONEpidermal growth factor receptor (EGFR) is an important target in the process of reducing tumor growth. More than 60% of non-small cell lung cancers express EGFR [1]. EGFR downstream signaling increases proliferation and angiogenesis and reduces apoptosis [2]. The overexpression of EGFR has been detected in the majority of human carcinomas [3].Tyrosine kinase inhibitors (TKIs) are one of four classes of anti-EGFR agents used for cancer therapy [4]. In 2015, gefitinib (one of the TKIs) was approved by the Food and Drug Administration (FDA) as an inhibitor of EGFR [5]. In addition to synthetic drugs, natural products have an important role in cancer therapy. Chemical compounds from natural products can be potential new medicines. The exploration of bioactive compounds from marine materials, including marine fungi, has become a major activity lately for anticancer treatment. Currently, there are anticancer compounds from marine fungal metabolites that have entered clinical trials. One of the alkaloid compounds, plinabulin, is a modified structure based on the (-)-phenylahistin metabolites of the terrestrial and marine fungi Aspergillus ustus [6]. As the development of microbiology technology increases in the future, the availability of microorganisms such as marine fungi will increase and the costs of production will be reduced. Therefore, bioactive compounds of marine fungi have the potential to be new drug compounds [7]. A database of active compounds was created to ease in silico and in vitro research further.In silico research can support conventional research (wet lab) that is relatively expensive and takes time [8]. AutoDock (Automatic Docking) is widely used to predict the complex of a biomolecular structure or to perform functional analysis and molecular design. AutoDock uses a grid-based method to rapidly evalu...

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Immense Essence of Excellence: Marine Microbial Bioactive Compounds

Cited by 336 publications

References 117 publications

A novel halo-alkali-tolerant and thermo-tolerant chitinase from Pseudoalteromonas sp. DC14 isolated from the Caspian Sea

A novel halo-alkali-tolerant and thermo-tolerant chitinase from Pseudoalteromonas sp. DC14 isolated from the Caspian Sea

Antiangiogenic, wound healing and antioxidant activity ofCladosporium cladosporioides(Endophytic Fungus) isolated from seaweed (Sargassum wightii)

Database Compilation and Virtual Screening of Secondary Metabolites Derived From Marine Fungi as Epidermal Growth Factor Receptor Tyrosine Abstract Kinase Inhibitors

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