Immersion in 6-Benzylaminopurine for Dormancy Release and Initiation  of Potato Sprouts at Various Tuber Weight and Storage Duration

Siregar, Luthfi Aziz Mahmud; Turnip, L; Damanik, Idamayanti

doi:10.24831/jai.v49i1.34374

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…In the present investigation, sprouts began to emerge after week 8; that is, a latency period was observed, unlike what has been reported by other authors (Melo et al, 2021;Siregar et al, 2021). Torres et al (2011) state that this period lasts between 2 and 14 wk.…”

Section: Physiological Quality Of Potato Seed Tuberscontrasting

confidence: 79%

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Physiological and starch quality of potato tubers discarded from the potato chip industry in Mexico

Montoya-Anaya,

Aguirre-Mancilla,

Rodríguez-Núñez

et al. 2024

Chil. j. agric. res.

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Agroindustrial potato (Solanum tuberosum L.) waste mainly includes peel and pulp; however, potatoes of low industrial quality are also discarded by industry. A viable alternative to give added value to these byproducts is the use of these as seeds, as well as the use of starch. The objective of this work was to evaluate the physiological quality (germination and vigor), starch content and relationship between these variables in discarded tubers from the industry through chemical-structural analyses to better understand their influence on starch composition. The physiological quality of the potatoes was evaluated through germination and vigor tests; likewise, starch was extracted, and the yield was determined. Physicochemical characterization of the starches was carried out through proximal and structural analysis using nuclear magnetic resonance (NMR). The results showed that the tubers had 100.00% germination and 98.66% vigor and were suitable for use as seeds. The starch yield did not significantly differ. However, characterization of the starch revealed significant differences (p ≤ 0.05) in moisture, ash, carbohydrate and amylose contents, which were influenced by the storage temperature (4 °C) of the tubers subjected to a vigor test, which interferes with enzymatic activity. During germination, the NMR results showed type B crystallinity, which is typical of tubers, indicating that the viability tests did not promote changes in the type of crystallinity. The results obtained can help us understand the effect of germination on the composition of starch and, accordingly, choose the most appropriate applications for this starch, whether food or nonfood.

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Section: Physiological Quality Of Potato Seed Tuberscontrasting

confidence: 79%

“…Melo et al (2021) reported germination percentages of 65%-94% and an average number of sprouts per potato of 3-11 depending on the genotype used over a period of 4 wk. On the other hand, Siregar et al (2021) obtained 91.67% germination, with an average number of sprouts per potato of 1.79 at 5 wk.…”

Section: Physiological Quality Of Potato Seed Tubersmentioning

confidence: 93%

Physiological and starch quality of potato tubers discarded from the potato chip industry in Mexico

Montoya-Anaya,

Aguirre-Mancilla,

Rodríguez-Núñez

et al. 2024

Chil. j. agric. res.

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“…An abrupt increase in the concentration of endogenous zeatin (naturally occurring CK) and a reduction in ABA at the onset of dormancy breakage have been observed [21]. Both natural (such as zeatin) and synthetic CK (such as kinetin and 6-benzyladenine) applications can rapidly trigger tuber sprouting [22,23]. GA and CK were reported to act synergistically to accelerate sprouting (reviewed by Kolachevskaya et al) [24].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analyses Reveal the Role of Cytokinin and the Nodal Stem in Microtuber Sprouting in Potato (Solanum tuberosum L.)

Zhang,

Fujino,

Shimura

2023

IJMS

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In potatoes, tuber secondary growth, especially sprouting, deforms the tubers and severely lowers their commercial value. Tuber sprouting is induced by signal substances, such as gibberellin (GA), which are transported to the tuber from the plant body. The molecular mechanism underlying GA-induced sprouting remains ambiguous. Here, we tried to recreate tuber secondary growth using in vitro stemmed microtubers (MTs) (with the nodal stem attached) and MT halves (with the nodal stem entirely removed). Our experiments showed that GA alone could initiate the sprouting of stemmed microtubers; however, GA failed to initiate MT halves unless 6-benzyladenine, a synthetic cytokinin CK, was co-applied. Here, we analyzed the transcriptional profiles of sprouting buds using these in vitro MTs. RNA-seq analysis revealed a downregulation of cytokinin-activated signaling but an upregulation of the “Zeatin biosynthesis” pathway, as shown by increased expression of CYP735A, CISZOG, and UGT85A1 in sprouting buds; additionally, the upregulation of genes, such as IAA15, IAA22, and SAUR50, associated with auxin-activated signaling and one abscisic acid (ABA) negative regulator, PLY4, plays a vital role during sprouting growth. Our findings indicate that the role of the nodal stem is synonymous with CK in sprouting growth, suggesting that CK signaling and homeostasis are critical to supporting GA-induced sprouting. To effectively control tuber sprouting, more effort is required to be devoted to these critical genes.

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Methods to Induce Sprouting in Dormant Potato Tubers for Direct Tuber Testing of Potato Virus Y

Gelles,

Olsen,

Thornton

et al. 2024

Am. J. Potato Res.

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The ability to initiate sprouting soon after harvest to enable direct tuber testing for potato virus Y (PVY) could aid in acquiring more rapid results compared to the traditional winter grow out tests currently used. Methods to break dormancy for PVY detection using laboratory direct tuber testing by ELISA in commercially produced Ranger Russet, Clearwater Russet, and Umatilla Russet seed lots were tested over two years and compared to leaf testing results obtained from the winter grow out and spring grow out. At harvest, three 400 tuber samples from each cultivar were obtained for the trial and included (1) untreated control (UTC), (2) application of cold aerosol smoke, or (3) application of Rindite. Tuber samples were held at 18.3 C and sprout development was monitored weekly. Treatments were direct tuber tested for PVY when one treatment of that cultivar achieved three sprouts elongating to six millimeters. A fourth 400 tuber sample was collected, treated with Rindite, and included in the Idaho winter grow out plots in Waialua, Hawaii and leaves were sampled and evaluated for PVY using ELISA. Laboratory tested seed was stored and planted in a spring grow out (Kimberly, Idaho) and leaf samples were analyzed for PVY by ELISA. Rindite treated tubers had greater sprout rating and number of sprouts elongating compared to UTC tubers and tubers receiving the smoke treatment at time of PVY testing. Smoke had a greater sprout rating but did not always significantly differ in the number of sprouts elongating compared to the untreated tubers. Overall, estimates of PVY prevalence from direct tuber testing showed limited significant differences to those obtained in the winter grow out for each cultivar, year, and PVY incidence. However, in year two, the incidence of PVY in the winter grow out (7% PVY) significantly differed from direct tuber testing (16% PVY) in Ranger Russet. In both years, the spring grow out PVY results for all cultivars were not significantly different than the direct tuber testing, except in year one the Ranger Russet direct tuber tested UTC showed 10% lower PVY detection compared to the spring grow out. This study identified a novel dormancy breaking treatment to promote earlier and accurate PVY detection by direct tuber testing using ELISA and provided data to support direct tuber testing for post-harvest evaluation of PVY in seed certification.

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Immersion in 6-Benzylaminopurine for Dormancy Release and Initiation of Potato Sprouts at Various Tuber Weight and Storage Duration

Cited by 4 publications

References 14 publications

Physiological and starch quality of potato tubers discarded from the potato chip industry in Mexico

Physiological and starch quality of potato tubers discarded from the potato chip industry in Mexico

Transcriptomic Analyses Reveal the Role of Cytokinin and the Nodal Stem in Microtuber Sprouting in Potato (Solanum tuberosum L.)

Methods to Induce Sprouting in Dormant Potato Tubers for Direct Tuber Testing of Potato Virus Y

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