Immersion optical clearing of adipose tissue in rats: ex vivo and in vivo studies

Yanina, Irina Yu.; Tanikawa, Yohei; Genina, Elina A.; Dyachenko, Polina A.; Tuchina, Daria K.; Bashkatov, Alexey N.; Dolotov, Leonid E.; Tarakanchikova, Yana V.; Terentuk, Georgy S; Navolokin, Nikita A.; Bucharskaya, Alla B.; Maslyakova, Gаlina N.; Iga, Yasunobu; Takimoto, Shinichi; Tuchin, Valery V.

doi:10.1002/jbio.202100393

Cited by 6 publications

(2 citation statements)

References 68 publications

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“…Diatrizoic acid is an iodinated aromatic that is often used as a radiocontrast agent, and it can also induce mitophagy and oxidative stress via calcium dysregulation [ 54 ]. Studies have demonstrated that diatrizoic acid is a component of FocusClear™, which has been used for optical clearing of mouse brain, skin, bone, and lymph node cortex tissues [ 55 , 56 ]. Moreover, oral meglumine diatrizoate esophagography could screen esophageal fistulas during radiotherapy or chemoradiotherapy with minimal side effects [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Expression Profiles of Long Non-Coding RNAs in the Articular Cartilage of Rats Exposed to T-2 Toxin

Yu,

Wang,

Luo

et al. 2023

IJMS

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T-2 toxin could induce bone damage. But there is no specific mechanism about the long non-coding RNAs (lncRNAs) involved in T-2 toxin-induced articular cartilage injury. In this study, 24 SD rats were randomly divided into a control group and a T-2 group, which were administered 4% absolute ethanol and 100 ng/g · bw/day of T-2 toxin, respectively. After treatment for 4 weeks, safranin O/fast green staining identified the pathological changes in the articular cartilage of rats, and immunofluorescence verified the autophagy level increase in the T-2 group. Total RNA was isolated, and high-throughput sequencing was performed. A total of 620 differentially expressed lncRNAs (DE-lncRNAs) were identified, and 326 target genes were predicted. Enrichment analyses showed that the target genes of DE-lncRNAs were enriched in the autophagy-related biological processes and pathways. According to the autophagy database, a total of 23 autophagy-related genes were identified, and five hub genes (Foxo3, Foxo1, Stk11, Hdac4, and Rela) were screened using the Maximal Clique Centrality algorithm. The Human Protein Atlas database indicated that Rela and Hdac4 proteins were highly expressed in the bone marrow tissue, while Foxo3, Foxo1, and Stk11 proteins were reduced. According to Enrichr, etoposide and diatrizoic acid were identified as the key drugs. The real-time quantitative PCR results were consistent with the RNA sequencing (RNA-Seq) results. These results suggested that autophagy was involved in the rat articular cartilage lesions induced by T-2 toxin. The lncRNAs of NONRATG014223.2, NONRATG012484.2, NONRATG021591.2, NONRATG024691.2, and NONRATG002808.2, and their target genes of Foxo3, Foxo1, Stk11, Hdac4, and Rela, respectively, were the key regulator factors of autophagy.

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Section: Discussionmentioning

confidence: 99%

Expression Profiles of Long Non-Coding RNAs in the Articular Cartilage of Rats Exposed to T-2 Toxin

Yu,

Wang,

Luo

et al. 2023

IJMS

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“…The use of DMSO, sonophoresis, laser irradiation, microdermabrasion, microperforation and other enhancers of OCA penetration in tissues are described in Refs. [ 38 , 39 , 40 , 41 , 42 ]. It is important for the development of these approaches to provide tissue viability.…”

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confidence: 99%

Tissue Optical Clearing: State of the Art and Prospects

Genina

2022

Diagnostics

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Influence of optical clearing agents on the scattering properties of human nail bed and blood microrheological properties: In vivo and in vitro study

Moldon,

Ermolinskiy,

Lugovtsov

et al. 2024

Journal of Biophotonics

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Optical clearing agents (OCAs) are substances that temporarily modify tissue's optical properties, enabling better imaging and light penetration. This study aimed to assess the impact of OCAs on the nail bed and blood using in vivo and in vitro optical methods. In the in vivo part, OCAs were applied to the nail bed, and optical coherence tomography and optical digital capillaroscopy were used to evaluate their effects on optical clearing and capillary blood flow, respectively. In the in vitro part, the collected blood samples were incubated with the OCA and blood aggregation properties were estimated using diffuse light scattering techniques. The results indicate that OCAs significantly influence the optical properties of the nail bed and blood microrheology. These findings suggest that OCAs hold promise for improving optical imaging and diagnostics, particularly for nail bed applications, and can modify blood microrheology.

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Immersion optical clearing of adipose tissue in rats: ex vivo and in vivo studies

Cited by 6 publications

References 68 publications

Expression Profiles of Long Non-Coding RNAs in the Articular Cartilage of Rats Exposed to T-2 Toxin

Expression Profiles of Long Non-Coding RNAs in the Articular Cartilage of Rats Exposed to T-2 Toxin

Tissue Optical Clearing: State of the Art and Prospects

Influence of optical clearing agents on the scattering properties of human nail bed and blood microrheological properties: In vivo and in vitro study

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