Immmunophenotype of Spontaneous Hematolymphoid Tumors Occurring in Young and Aging Female CD-1 Mice

Abstract

A few reports indicated the incidence of hematolymphoid neoplasms in old CD-1 mice, but the cellular lineage of CD-1 mouse neoplasms has not be published. In this study, immunohistochemistry (IHC) was used to characterize the cellular lineage of spontaneous hematolymphoid neoplasms arising in young female CD-1 mice used as health monitoring sentinels and aging female CD-1 mice used as controls in 80 wk carcinogenesis studies. Lymphoblastic lymphomas of T-cell and B-cell lineage were common in mice 12 mo or less of age, whereas a wide range of non-lymphoblastic B-cell lymphomas and lymphoblastic T-cell lymphomas were common in mice > 12 mo old. Renal hyaline droplets positive for lysozyme were observed in aged mice with a histiocytic-associated large B-cell lymphoma (HA-BCL) and a myeloid leukemia. Endogenous ecotropic MuLV genes have been recovered from CD-1 mice, but MuLV protein expression has not been previously demonstrated. We reported for the first time the expression of MuLV protein by IHC in lymphomas and some normal tissues of both young and aging CD-1 mice. This report should help to differentiate spontaneous lymphomas and leukemias in CD-1 mice from those induced by chemicals and other methods.

Conditioning Regimens in Long-Term Pre-Clinical Studies to Support Development of Ex Vivo Gene Therapy: Review of Nonproliferative and Proliferative Changes

“…1d). 19,20 Additional lesions attributed to TBI were observed in the reproductive system and the lens. Lenticular degeneration was observed up to a marked severity in most animals from all studies (Supplementary Fig.…”

Finding mouse models of human lymphomas and leukemia's using the Jackson laboratory mouse tumor biology database

“…The listings of lymphomas in Table 1 are the original diagnoses provided by the pathologist or scientist contributing them and may not agree with current established criteria. With today’s classification (Morse et al, 2002; Rehg et al, [Epub ahead of print]), they may be classified differently. The new Lymphoma Resource page allows the user the ability to compare the data for a specific mouse lymphoma model to the histopathology and immunohistochemical labeling patterns listed to help determine the exact lymphoma sub-type.…”

Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice

“…The p30 antibody has been previously published as being cross-reactive with endogenous retroviruses, specifically endogenous murine leukemia viruses. 23,46,53 CD1 mouse tissues were used as a positive control for interpreting p30 labeling. 53 C57BL/6 (B6) mouse tissues were used as negative controls for the p30 antibody since B6 mice have been reported to have undetectable ecotropic MuLV.…”