Immobilisation of DNA to polymerised SU-8 photoresist

Marie, Rodolphe; Schmid, Silvan; Johansson, Alicia; Ejsing, Louise; Nordström, Maria; Häfliger, Daniel; Christensen, Claus Bo Vöge; Boisen, Anja; Dufva, Martin

doi:10.1016/j.bios.2005.03.004

Cited by 85 publications

(100 citation statements)

References 20 publications

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“…Investigators have described fluorescence measurements across the green to infrared wavelengths within systems constructed of SU-8, the polymer from which the pallets are formed (37)(38)(39). Reports by other investigators suggest that SU-8 possesses an auto-fluorescence that might hamper fluorescence imaging of cells on the pallets (40). To determine the properties of the SU-8 auto-fluorescence, we measured the emission spectra of SU-8 when excited at 400 nm.…”

Section: Fluorescence Of Su-8mentioning

confidence: 99%

Broadening cell selection criteria with micropallet arrays of adherent cells

et al. 2007

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A host of technologies exists for the separation of living, nonadherent cells, with separation decisions typically based on fluorescence or immunolabeling of cells. Methods to separate adherent cells as well as to broaden the range of possible sorting criteria would be of high value and complementary to existing strategies. Cells were cultured on arrays of releasable pallets. The arrays were screened and individual cell(s)/pallets were released and collected. Conventional fluorescence and immunolabeling of cells were compatible with the pallet arrays, as were separations based on gene expression. By varying the size of the pallet and the number of cells cultured on the array, single cells or clonal colonies of cells were isolated from a heterogeneous population. Since cells remained adherent throughout the isolation process, separations based on morphologic characteristics, for example cell shape, were feasible. Repeated measurements of each cell in an array were performed permitting the selection of cells based on their temporal behavior, e.g. growth rate. The pallet array system provides the flexibility to select and collect adherent cells based on phenotypic and temporal criteria and other characteristics not accessible by alternative methods. ' 2007 International Society for Analytical Cytology

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Section: Fluorescence Of Su-8mentioning

confidence: 99%

Broadening cell selection criteria with micropallet arrays of adherent cells

et al. 2007

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“…Furthermore, it should be amenable to subsequent immobilization of molecules. We use the novolacepoxy resin SU8 (a negative resist), which is widely used for microfabrication and micropatterning [11][12][13] and as a support material for the direct attachment of biomolecules [14][15][16][17][18]. We have previously used SU8 to manufacture micronmetresized bars (i.e.…”

Section: Introductionmentioning

confidence: 99%

Holographically encoded microparticles for bead-based assays

Birtwell

Banu

Zheludev

et al. 2009

J. Phys. D: Appl. Phys.

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We demonstrate a re-writable, high capacity holographic encoding technique for multiplexed bead-based suspension assays. The microparticles are made from SU8 doped with a photochromic diarylethene dye and manufactured using multilayer photolithography and dry etching. Each particle is encoded with a unique hologram, whose diffraction pattern consists of bright and dark regions, representing a binary number that identifies the particle. Theoretically up to 10 24 unique codes are available on a 100 µm particle using this method, when the code is read with a standard 2/3 CMOS camera. Encoding capacities of 512 unique codes have been demonstrated on a 500 µm SU8 particle. The code is thermally stable for 3 days at 25 • C, and once written, the code can be erased and re-written once whilst still remaining readable. The code can be written into the particle during an assay experiment (no pre-encoding is required) and requires simple optics for reading.

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“…16 In this paper we describe, for the first time, the preparation and surface modification of SU-8 particles shaped as microbars, the attachment of appropriate spacers in combination with linkers and the successful application of these particles to multistep synthesis leading to oligonucleotides and peptides attached in an unambiguous manner to the support surface. While SU-8 will probably not become a successful support for synthesis on its own right competing with optimized supports for synthesis, its establishment in microfabrication coupled with the feasibility of carrying out multistep synthesis enables access to interesting applications in bioanalytical sciences (e.g.…”

Section: Introductionmentioning

confidence: 99%

Multistep Synthesis on SU-8: Combining Microfabrication and Solid-Phase Chemistry on a Single Material

et al. 2007

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SU-8 is an epoxy-novolac resin and a well-established negative photoresist for microfabrication and microengineering. The photopolymerized resist is an extremely highly crosslinked polymer showing outstanding chemical and physical robustness with residual surface epoxy groups amenable for chemical functionalization. In this paper we describe, for the first time, the preparation and surface modification of SU-8 particles shaped as microbars, the attachment of appropriate linkers, and the successful application of these particles to multistep solid-phase synthesis leading to oligonucleotides and peptides attached in an unambiguous manner to the support surface.

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Immobilisation of DNA to polymerised SU-8 photoresist

Cited by 85 publications

References 20 publications

Broadening cell selection criteria with micropallet arrays of adherent cells

Broadening cell selection criteria with micropallet arrays of adherent cells

Holographically encoded microparticles for bead-based assays

Multistep Synthesis on SU-8: Combining Microfabrication and Solid-Phase Chemistry on a Single Material

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