Immobilisation of yeast cells on the surface of hydroxyapatite ceramics

Rapoport, Alexander; Borovikova, Diāna; Kokina, Agnese; Patmalnieks, Aloizijs; Polyak, Nataliya; Pavlovska, Ilona; Mežinskis, Gundars; Dekhtyar, Yury

doi:10.1016/j.procbio.2010.11.009

Cited by 36 publications

(17 citation statements)

References 26 publications

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“…Conversely, attachment of cells to a carrier surface is less expensive due to simplicity of the technique and cheap carriers, which are often used for this purpose, but at the same time it provides unstable immobilization of cells that can be easily detached from the carrier during industrial processes [9][10][11]. Using a newer immobilization method, recently developed in our laboratory [20], the current study was able to demonstrate high levels of stable cell immobilization using relatively simple and inexpensive methods. The peak amount of ethanol produced (48 mg/mL) did not differ from free yeast cells.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, for the described experiments, cells for the immobilization process were prepared in distilled water. The immobilization method used in the current study was recently developed by our research group and is unique because it involves an additional dehydration stage [20]. Yeasts immobilized by this newer method were determined to possess high biotechnological efficiency, which was evaluated based on heavy metal biosorption activity [20].…”

Section: Immobilization Of Yeast Cells On Ceramic Carriersmentioning

confidence: 99%

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Effects of yeast immobilization on bioethanol production

Borovikova

Scherbaka

Patmalnieks

et al. 2014

Biotech and App Biochem

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The current study evaluated a newer method, which includes a dehydration step, of immobilizing Saccharomyces cerevisiae L-77 and S. cerevisiae L-73 onto hydroxylapatite and chamotte ceramic supports. The efficiency of cell immobilization on chamotte was significantly higher than hydroxylapatite. Immobilized yeast preparations were investigated for their ethanol-producing capabilities. The glucose concentration in a fermentation medium was 100 mg/mL. Immobilized preparations produced the same amount of ethanol (48 ± 0.5 mg/mL) as free cells after 36 H of fermentation. During the early stages of fermentation, immobilized yeast cells produced ethanol at a higher rate than free cells. Yeast preparations immobilized on both supports (hydroxylapatite and chamotte) were successfully used in six sequential batch fermentations without any loss of activity. The chamotte support was more stable in the fermentation medium during these six cycles of ethanol production. In addition to the high level of ethanol produced by cells immobilized on chamotte, the stability of this support and its low cost make it a promising material for biotechnologies associated with ethanol production.

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Section: Discussionmentioning

confidence: 99%

Section: Immobilization Of Yeast Cells On Ceramic Carriersmentioning

confidence: 99%

Effects of yeast immobilization on bioethanol production

Borovikova

Scherbaka

Patmalnieks

et al. 2014

Biotech and App Biochem

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“…Perlite—as the ceramic bead—was thoroughly washed with distilled water and then dried using an electrical oven at 180 °C for 3 hr; it was thereafter autoclaved (121 °C for 20 min). Yeast suspension (150 mL) was added to the ceramic support (250 mL) and transformed in rotary incubator shaker at 30 °C for 24 and 48 hr (Rapoport et al., ). After the binding, the ceramic particles were separated and washed with distilled water; there was no chemical modification on the ceramic.…”

Section: Methodsmentioning

confidence: 99%

Immobilization of Saccharomyces cerevisiae on Perlite Beads for the Decontamination of Aflatoxin M1 in Milk

Foroughi¹,

Sarabi-Jamab²,

Keramat³

et al. 2018

Journal of Food Science

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The immobilization of Saccharomyces cerevisiae cells on perlite beads can be used to detoxify AFM1-contaminated milk. The perlite can provide a perfect support for immobilization. With respect to qualitative properties, 20 min, was suggested as the optimum time for milk decontamination. This study indicated that the detoxification of contaminated milk using immobilized S. cerevisiae cells on the perlite support did not affect the different properties of detoxified milk. This method can lead to a practical solution to address aflatoxin contamination in dairy products considered high-risk foods.

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“…The ceramic beads were washed with distilled water and dried at 180°C for 3 hr, and then sterilized (120°C for 20 min). Then the ceramic supports (the number of porous activated alumina and or alumina silicate particles which were equivalent with 250 mL) were added to 150 mL viable yeast suspensions and transformed in rotary incubator shaker at 30°C for 24 and 48 hr (Rapoport et al ). After the immobilization process, the ceramic beads were withdrawn and washed with distilled water without any chemical modification on the ceramics.…”

Section: Methodsmentioning

confidence: 99%

The use ofSaccharomyces cerevisiaeimmobilized on activated alumina, and alumina silicate beads for the reduction of Aflatoxin M₁in vitro

Foroughi¹,

Sarabi-Jamab²,

Keramat³

et al. 2019

J Food Process Preserv

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This study was aimed to investigate the immobilization of Saccharomyces cerevisiae on two different types of ceramic beads (activated alumina and alumina silicate) and comparing the supports for reduction aflatoxin M1 (AFM1) in vitro. The immobilization of S. cerevisiae was done on ceramic beads for 24 and 48 hr. The best immobilization was achieved at 48 hr. The images of scanning electron microscope confirmed that the immobilization of yeast cells on the activated alumina was better than the alumina silicate (P < 0.05). The ceramic beads with immobilized yeasts were applied to remove the AFM1 from contaminated standard liquid (0.2 µg/L) by 5, 10, and 20 min circulation time. Results showed that the AFM1 concentration in the solution after 20 min circulation was minimal and the highest AFM1 reduction was 75%, when the contaminated solution passed through the activated alumina beads. There was no Si or Al residue in standard liquid. Practical applications This study indicated that the activated alumina and alumina silicate can provide a perfect support for immobilization and the immobilization of Saccharomyces cerevisiae cells on ceramic beads can be used practically to detoxify AFM1‐contaminated liquid food.

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Immobilisation of yeast cells on the surface of hydroxyapatite ceramics

Cited by 36 publications

References 26 publications

Effects of yeast immobilization on bioethanol production

Effects of yeast immobilization on bioethanol production

Immobilization of Saccharomyces cerevisiae on Perlite Beads for the Decontamination of Aflatoxin M1 in Milk

The use ofSaccharomyces cerevisiaeimmobilized on activated alumina, and alumina silicate beads for the reduction of Aflatoxin M₁in vitro

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Immobilisation of yeast cells on the surface of hydroxyapatite ceramics

Cited by 36 publications

References 26 publications

Effects of yeast immobilization on bioethanol production

Effects of yeast immobilization on bioethanol production

Immobilization of Saccharomyces cerevisiae on Perlite Beads for the Decontamination of Aflatoxin M1 in Milk

The use ofSaccharomyces cerevisiaeimmobilized on activated alumina, and alumina silicate beads for the reduction of Aflatoxin M1in vitro

Contact Info

Product

Resources

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The use ofSaccharomyces cerevisiaeimmobilized on activated alumina, and alumina silicate beads for the reduction of Aflatoxin M₁in vitro