Immobilised Metal Affinity Chromatography (Imac) Beads for Lysozyme Separation: Synthesis and Characterization Study

Abstract

Immobilized metal affinity chromatography (IMAC) has been established as a highly specific chromatographic technique for the production of enzymes and proteins including lysozyme. This study aimed to prepare and characterize the IMAC beads for lysozyme separation. Silica gel served as chromatographic matrix which has been coated with chitosan layer and crosslinked with glutaraldehyde (GTA-CTS-SiO 2 ) since it is very convenient to promote fixation. Various IMAC ligands were immobilized by chelating 1500 mg/l Cu , Fe 3+ and Al 3+ ions, respectively on GTA-CTS-SiO 2 . IMAC which was immobilized with 1200mg/l Cu 2+ exhibited the maximal immobilization capacity (27.08mg/g) of within 30 minutes incubation time. This fundamental study can be a momentous pathway to develop an efficient chromatographic system for lysozyme separation in the future.Keywords: silica gel, chitosan, glutaraldehyde, metal ions, copper (II) ion Abstrak Kromatografi afiniti logam dipegun (IMAC) telah diperlihatkan sebagai satu teknik kromatografi yang sangat spesifik untuk pengeluaran enzim dan protein termasuk lysozim. Kajian ini bertujuan untuk menyediakan dan mencirikan manik IMAC bagi tujuan pemisahan lysozim. Gel silika berperanan sebagai sokongan kepada kromatografi yang telah dilapisi dengan lapisan kitosan dan diaktifkan dengan glutaraldehid (GTA-CTS-SiO 2 ) kerana kesesuaiannya yang berupaya menggalakkan lekatan.

Anthropogenic determination and risk assessment of total petroleum hydrocarbons of ground water within Orji auto repair workshops, Owerri, Imo State, Nigeria

“…Petroleum hydrocarbons in organisms and exposed individuals often result in numerous health implications such as: disruption in the activities of various body organs, severe damage to the pancreas, kidney, liver, blood circulatory system, and ultimately death [12]. The aim of this research is to determine the concentration of total petroleum hydrocarbons in different groundwater samples in Orji auto repair workshops and also to assess the risk posed by consumption of such polluted water [18][19][20]27].…”