Immobilization: A Cause of Resorptive Hypercalciuria

Jeantet, A; Giachino, G; Rossi, Paulo Giusti; Tetta, Cecilia; Thea, A; Squiccimarro, G; Calitri, V; Vercellone, A

doi:10.1159/000408545

Cited by 4 publications

(3 citation statements)

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“…SDS-PAGE was performed ta~iag the method of Laemmli [35] with the mod~ticat~ons described by Melki ctal. [28] in order to inc~ea,~c the separation of the ct andfl ~ubunits of tubalin 1EF was carried oat in cylindrical gels and the presence of 9 5 M urea, as described by Wolff' et al [36], e~cept that pH 5.0-5.5 ampholyte~ (Serva) were used. Gels were stained with 0 2% Coomassie brilliant blue R-250 in 5% accuc acid and 50% ethanol m water.…”

Section: Sgs-polyacr Ylamid Gel E~ectrophorests and Tsoelectrw Focumentioning

confidence: 99%

Structure of tubulin C‐terminal domain obtained by subtilisin treatment The major α and β tubulin isotypes from pig brain are glutamylated

Redeker¹,

et al. 1992

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Limited ~ubtihsin digestion of the tubulin ~, fl heterodlmei ha~ been used in this ~vork to reduce the total number of tubulin isotypes from 20 for native to 9 re1 bubtdma.¢lcaved tubuhn This indicalc~ that ~he major part of tul~uhn h,*terogenelty is located at the C.terminus of the molecule. The C-terminal pcpfides of both a and fl ~ubumt~ of tt,buhn ~.ere purified by anion-enchange HPLC. Combined tt~e of Edman desradation chemistry and mass spectrometry on the isolated peptlde~ shown that subtdi~in cleavase occurs at posmon Asp-438 and His-406 of ct and Gin-433 and His.396 o1"/3 tubuhn chums. Quaiahtat~ve analysis of our data show that cleavage at po~it~ons His.406 (~) and I-1is-396 (.8) occurs with a low efficient), an~l indicates that the naajor i~otylgeS of P~8 brain tubulm are modified by sequemial altachmeat of I to 5 glutam~c acid residues at posit~ons or -435 of ¢ and/3 tubuhn, respectively

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Section: Sgs-polyacr Ylamid Gel E~ectrophorests and Tsoelectrw Focumentioning

confidence: 99%

Structure of tubulin C‐terminal domain obtained by subtilisin treatment The major α and β tubulin isotypes from pig brain are glutamylated

Redeker¹,

et al. 1992

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“…This is further compounded by a higher incidence of peripheral vasculopathy and other cardiac morbidities in patients with DM, as with our participants. Immobilization induces increased bone turnover and leads to resorptive hypercalciuria 22. Second, frail individuals are often encouraged to increase their nutrient intake to avoid negative protein balance,23 and this high dietary protein intake significantly increases the probability of calciuria and renal stones 24.…”

Section: Discussionmentioning

confidence: 99%

Frailty predicts a higher risk of incident urolithiasis in 525 368 patients with diabetes mellitus: a population-based study

Chao¹,

Wang

Hung³

et al. 2020

BMJ Open Diab Res Care

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ObjectivePatients with diabetes have an increased risk for urolithiasis, but the associated risk factors remain an active area of research. We investigated whether frailty influenced the probability of patients with diabetes developing urolithiasis.Research design and methodsUsing data from the Longitudinal Cohort of Diabetic Patients from 2004 to 2010, we identified those without and with frailty based on a validated, modified FRAIL scale. Patients were followed until they developed urolithiasis, and we used Kaplan-Meier and Cox proportional hazard regression analyses to examine the relationship between frailty, its severity, and the risk of urolithiasis, accounting for demographic profiles, comorbidities, frailty status changes over follow-up, and medications, with risk competition by mortality.ResultsAmong 525 368 patients with diabetes, 64.4% were not frail, while 28.5%, 6.6%, and 0.6% had 1, 2, and ≥3 FRAIL items at baseline. After 4.2 years of follow-up, 13.4% experienced incident urolithiasis. Cox proportional hazard regression analysis showed that patients with diabetes having at least one FRAIL criterion exhibited a significantly higher risk for urolithiasis compared with non-frail patients (for 1, 2, and ≥3 items, hazard ratio (HR)s: 1.04, 1.23, and 1.46; 95% confidence intervals (CIs) 0.99 to 1.09, 1.12 to 1.35, and 1.12 to 1.91, respectively). This increase in urolithiasis risk remained significant if we restricted analyses to renal stones or recurrent urolithiasis as the study outcomes.ConclusionsFrailty may pose a risk for incident urolithiasis in patients with diabetes. Treating frailty may potentially reduce their risk for urolithiasis.

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“…Obesity is demonstrated as a risk factor for the formation of calcium oxalate and uric acid stones in the elderly, according to findings from comprehensive cohort studies such as the Amirkola Health and Ageing Project (AHAP) [22]. Chronic immobility also affects bone metabolism, with increased bone turnover eventually leading to a state of resorptive hypercalciuria [23]. Another explanation for increased risks of developing urolithiasis amongst older and frail individuals likely relates to their dietary patterns.…”

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confidence: 99%

To What Extent Does Frailty Influence the Risk of Developing Urolithiasis?

Chinnadurai

2022

Uro

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Urolithiasis has become more prevalent in recent years, given the rapid rise of the global geriatric population. Although factors such as ethnicity, dietary and fluid intake, co-morbidity status and age have been associated with increased incidence of urolithiasis, the links between frailty status and risks of developing urolithiasis are not yet known. In this commentary, we will explore the scale and significance of this relationship based on emerging evidence. We will review the plausible factors on how a more severe frailty status may be significantly associated with greater risks of developing urolithiasis. We will also discuss the strategies that may help to lower the incidence of urolithiasis in older and frail individuals. We hope our article will bring greater awareness on this issue and motivate further research initiatives evaluating the relationship between frailty and urolithiasis, as well as holistic prevention strategies to lower the risks of developing urolithiasis within this vulnerable population.

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Immobilization: A Cause of Resorptive Hypercalciuria

Cited by 4 publications

References 0 publications

Structure of tubulin C‐terminal domain obtained by subtilisin treatment The major α and β tubulin isotypes from pig brain are glutamylated

Structure of tubulin C‐terminal domain obtained by subtilisin treatment The major α and β tubulin isotypes from pig brain are glutamylated

Frailty predicts a higher risk of incident urolithiasis in 525 368 patients with diabetes mellitus: a population-based study

To What Extent Does Frailty Influence the Risk of Developing Urolithiasis?

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