Immobilization and Application of Partial Purified Lovastatin Produced From Local Isolate Aspergillus Terreus A50 Using Solid State Fermentation

AL-Sa’ady, Ali J. R.; Aziz, Ghazi M.

doi:10.36103/ijas.v52i2.1299

Cited by 5 publications

(5 citation statements)

References 20 publications

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“…sp. (F6) in concentrations of 20 , 40 and 60 %, a significant impact on reducing the germination rate of Ocimum seeds, as the germination rate of the three concentrations reached 84.77 IOP Publishing doi:10.1088/1755-1315/1262/3/032052 7 and 83.64% sequentially compared to the comparative treatment, in which the germination rate reached 87.47% (34,35,36). And I differ significantly with the coefficients of the filtrate of the fungus Alternaria sp.…”

Section: Testing the Efficiency Of Pathogenic Fungi Filtrate In Germi...mentioning

confidence: 98%

The Effect of Fungi Accompanying cuscuta on the Germination Rates of Ocimum and cuscuta Seeds and Their Molecular Diagnosis

Al-Zubaidi,

Al-Juboory

2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study was conducted at the Faculty of Agricultural Engineering Sciences / Plant Protection Department / University of Baghdad-Jadriya, with the aim of isolating fungal pathogens from samples of the parasite on different plant families (Solanum, Astragalus, Conocarpus, chenopodium, Alhagi, Ocimum) from several areas in Baghdad and Diyala Governorate and diagnosing them phenotypically and testing their pathogenicity. The results of isolation and phenotypic diagnostics from the stems and seeds of the cuscuta showed the presence of 12 genera belonging to nine species : Alternaria, sp. (F2), Alternaria sp. (F4), Aspergillus sp. (F6), Aspergillus sp. (F12), Chaetomium sp. (Amesia sp.) (F7), Cladosporium sp. (F8), Curvularia sp. (F5), Helminthosporium sp. (F10), Penicillium sp. (F9), Rhizoctonia solani (F1), Stemphylium sp. (F3), Stemphylium sp. (F11). The results showed that the fungus Aspergillus sp., Curvularia sp., Chaetomium sp. Helminthosporium sp. and SP. Stemphylium was first recorded on the Cuscuta plant in Iraq. The results of the pathogenetic assessment showed that all the tested isolates (F1-F12) were pathogenic to Porter and Ocimum seeds, as they caused a significant reduction in seed germination rates compared to the control treatment (without inoculation with fungal vaccine). it was found that there was a difference between fungal isolates in reducing the percentage of seed germination, as some fungal isolates achieved the highest reduction in the percentage of germination of carrier seeds. the effect of their fungal filters on the germination of Porter and Ocimum seeds was tested and diagnosed in part. The results showed that the leached fungus Chaetomium sp. With concentrations of 20, 40 and 60%, it significantly reduced the percentage of germination of Porter seeds and differed significantly with the coefficients of Aspergillus SP.(F6) fungal filters, Stemphylium sp.(F3), Alternaria sp.(F4), Curvularia sp.(F5) by analogy with the comparison treatment, while there are no significant differences between the fungal filtrate at the three concentrations. The results also showed that the fungus leached Chaetomium sp.(F7). And Aspergillus sp. sp.(F6) in concentration, it had a significant effect in reducing the percentage of germination of Ocimum seeds compared to the comparative treatment. She showed that the isolates belong to fungi (F2 Aspergillus Flavus and(F4) Alternaria alstroemeria and that F5 Curvularia spicifera and F3 Stemphylium vesicarium and F7 belong to Amesia atrobrunneum and the name was changed instead of the old name Chaetomium atrobrunneum and deposited in the gene bank Gene Bank and NCBI under the accession numbers oq746923, Oq746924, Oq746925 and Oq74692.

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Section: Testing the Efficiency Of Pathogenic Fungi Filtrate In Germi...mentioning

confidence: 98%

The Effect of Fungi Accompanying cuscuta on the Germination Rates of Ocimum and cuscuta Seeds and Their Molecular Diagnosis

Al-Zubaidi,

Al-Juboory

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Cytotoxicity was tested according to the method described by [27,28]. The MTT test was used to determine the cytotoxicity of β-CN protein hydrolyzates purified from camel milk on HepG2, PC3, and MCF-7 cells, this is done by placing the cell suspension at concentrations (1 x 10 4 -1 x 10 6 cells/ml) in a flat-bottomed 96-hole plate to a final volume of 200 microliters of complete culture medium for each hole, and then incubating in an incubator supplied with 5% CO 2 gas at a temperature of 37 Cº for 24 hours.…”

Section: Mtt Cytotoxicity Test To Examine Cell Viabilitymentioning

confidence: 99%

Evaluation of the Toxicological Efficacy of Protein Hydrolysates From Camel Milk β-Casein Against Different Types of Cancer Cell Lines in Vitro

Hadi Al-Shaikh,

Doosh

2024

IOP Conf. Ser.: Earth Environ. Sci.

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This study aimed to isolate and purify the β-CN protein from camel milk using ion exchange chromatography and gel filtration techniques and to verify its purity using polyacrylamide gel electrophoresis (PAGE), Then prepare hydrolyzates of β-CN isolated from camel milk by enzymatic hydrolysis using the enzyme pepsin and trypsin and their mixture in a ratio (1:1). The cytotoxicity of β-CN protein degraders was studied by conducting an MTT test to determine the toxic effect of β-CN degraders and their ability to inhibit the growth of cancer cells represented by HepG2 liver cancer cells, PC3 prostate cancer cells, and MCF-7 breast cancer cells and comparing them with normal HdFn cells in vitro. The results showed the presence of two peaks for the β-CN protein on DEAE-Cellulose and one peak on the Sephadex G-100 column and the appearance of a clear and pure protein band on the polyacrylamide gel. the highest decomposition rate was due to the synergistic action of the enzyme pepsin and trypsin at a ratio of 1:1 and amounted to 52.32%. The results also showed that β-CN protein hydrolyzates have an effective inhibitory effect on cancer cell growth at a concentration of 400µg/mL, as the inhibition rate reached (45.9, 59.5 and 63.3%) for the cell types, respectively. Based on the results of the MTT test, the MCF-7 cell line was chosen as it is the most affected by the β-CN degraders under study. Cytotoxicity was evaluated by performing an HCS test to detect some changes that may occur to cells by measuring cellular indicators, including the Viable Cell Count (VCC), total Nuclear Intensity (TNI), Cell Membrane Permeability (CMP), Mitochondrial Membrane Potential (MMP), and Cytochrome C releasing (CC) level. The results indicated a decrease in the number of (VCC) for MCF-7 cells, especially at concentrations of 100 and 200 µg/mL, by 31.53 and 47.58%, respectively, and an increase in (TNI), significantly at a significance level (P≤0.05), by 39.10 and 51.91%, respectively, there was a clear effect on (MMP) by 20.47 and 27.39%, respectively, and a significant increase in the level of Cytochrome C release by 16.16 and 25.84%, respectively. It can be concluded from the above that β-CN hydrolyzates have high inhibitory activity against the cancer cells under study in vitro.

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“…By incubating the chosen isolate for various amounts of time, the protease production parameter was first optimized (4,6,8,12,16,20,24,28,32,36,40,44, and 48 hours). Conical flasks were incubated for various amounts of time spaced (4 hours apart) to find the better incubation time for protease synthesis.…”

Section: Effect Of Incubation Timementioning

confidence: 99%

“…Despite the fact that E.coli is frequently utilized as a model pathogen and for the generation of recombinant proteins in the biotechnology sector [5], this is the case. Short bacilli, non-spore-forming, facultatively anaerobic, gram-negative bacteria [6], belonging to the Enterobacteriaceae family, and Escherichia coli are all characteristics of this organism [7]. It is produced on a basic media.…”

Section: Introductionmentioning

confidence: 99%

Biodisintegration of human mucin protein by protease produced from Escherichia coli AJ55 isolated from Urinary Tract Infection of Iraqi pa-tients

Alsa’ady,

Ibrahim,

Hassan

et al. 2023

Iraqi Journal of Science

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Proteases have various applications in the food, pharmaceutical, medicine, pathogenicity of some pathogenic bacteria, and detergent sectors as well as meeting the needs of approximately 60% of the global enzyme industry, whereas they catalyze the breakdown of protein molecules into peptides and amino acids. Production and purification of protease enzyme by the isolate Escherichia coli AJ55 was scrutinized in the present study. Cultivation optimum conditions, were various complex medium, carbon source, nitrogen source, temperature, pH of the medium, and time of incubation were optimized to enhance the total protease production in shake flask culture of E.coli AJ55. The nutrient broth supplemented with 2% glucose and 2% yeast extract, with a pH of 7.0 and incubated at 37 °C for 24 hours, better conditioned for producing the maximum production of protease. Escherichia coli AJ55 proteolytic enzyme was separated and purified using ion-exchange chromatography on a DEAE-Cellulose column and Sephadex G-150 gel after being precipitated with 0-70% saturated ammonium sulfate. Protease that had been partially purified had a yield of 34%, a purification fold of 13.4, an activity of 12.16 U/ml, a protein concentration of 0.005 mg/ml, and a specific activity of 2432 U/mg. By using gel filtration chromatography on a Sephadex G-150 gel, partially purified protease was examined for its ability to cleave the mucin protein. The findings of mucin biodegradation showed that the five fractions of the small peptides were produced after treatment of mucin with partially purified protease.

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Immobilization and Application of Partial Purified Lovastatin Produced From Local Isolate Aspergillus Terreus A50 Using Solid State Fermentation

Cited by 5 publications

References 20 publications

The Effect of Fungi Accompanying cuscuta on the Germination Rates of Ocimum and cuscuta Seeds and Their Molecular Diagnosis

The Effect of Fungi Accompanying cuscuta on the Germination Rates of Ocimum and cuscuta Seeds and Their Molecular Diagnosis

Evaluation of the Toxicological Efficacy of Protein Hydrolysates From Camel Milk β-Casein Against Different Types of Cancer Cell Lines in Vitro

Biodisintegration of human mucin protein by protease produced from Escherichia coli AJ55 isolated from Urinary Tract Infection of Iraqi pa-tients

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