Immobilization and long-term culturing of mouse embryonic stem cells in collagen-chitosan gel matrix

Abstract

We propose a method of creation of a 3D matrix consisting of native collagen fibers and natural polysaccharide chitosan. The collagen-chitosan hydrogels maintain viability and prolipherative activity of embryonic stem cells obtained from internal cells of mouse blastocyst. The proposed system forming hydrogels in situ can be used in cell therapy for immobilization and targeted delivery of stem cells.

Acceleration of diabetic wound healing with chitosan-crosslinked collagen sponge containing recombinant human acidic fibroblast growth factor in healing-impaired STZ diabetic rats

“…Several recent investigators have began to show the potential application of collagen/chitosan as matrices for liver tissue engineering (Wang et al, 2003, cartilage tissue engineering (Shi et al, 2005), periodontal tissue engineering (Zhang et al, 2006), skin-tissue engineering (Tangsadthakun et al, 2007), and even used for immobilization and target delivery of stem cells (Selezneva et al, 2006). To consistent with these recent studies, we provide the first evidence to show the successful use of CCCS/FGF for therapy of the healingimpaired diabetic skin wound.…”

Toxicity of iron oxide nanoparticles: Size and coating effects

“…Embryonic human fibroblasts were obtained by the method described previously . For experiments, we used cell culture on 8 to 10 passage.…”

Reduced hydraulic permeability of three-dimensional collagen scaffolds attenuates gel contraction and promotes the growth and differentiation of mesenchymal stem cells

“…While collagen gel contraction by fibroblasts has been extensively studied, few studies of gel contraction induced by cells of the osteogenic lineage have been reported (see, for example, [19,42,43]). Collagen gels with defined k values were seeded with MSCs in order to investigate long-term cell-scaffold interactions.…”