Immobilization-free photoelectrochemical aptasensor for environmental pollutants: Design, fabrication and mechanism

Sun, Caiqin; Liu, Meichuan; Sun, Huanhuan; Lu, Hanxing; Zhao, Guohua

doi:10.1016/j.bios.2019.111352

Cited by 47 publications

(21 citation statements)

References 48 publications

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“…However, Sun et al have recently reported using the full-length R12.23 aptamer in a photoelectrochemical aptasensor. 30 Unmodified R12.23 aptamer was adsorbed to graphene material to serve as the molecular beacon. The sensing principle depended upon the detachment of the aptamer from the graphene surface and the formation of the atrazine–aptamer complex.…”

Section: Resultsmentioning

confidence: 99%

In-Solution Molecular Recognition Comparison of Aptamers against the Herbicide Atrazine

2019

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Atrazine is a common herbicide that is widely used to control weed growth in both agricultural and residential settings. It has been shown to act as an endocrine disruptor that affects aquatic organisms. Rapid and low-cost monitoring methods for atrazine is the first step to mitigate its widespread persistency. Aptamers are small synthetic oligonucleotides that can assume a 3D structure to act as the molecular recognition element for a specific target of interest. Two different atrazine binding aptamers (R12.23 Trunc. and R12.45 Trunc.) have been identified from the same library design but with fundamentally different in vitro selection methodologies. While the R12.23 Trunc. has been utilized in immobilized biosensing platforms, it is unclear if in-solution-based applications would be suitable for both atrazine binding aptamers. This study provides the first insight of comparative in-solution binding profiles of the two atrazine binding aptamers. Based on our results, this information will be useful for future biosensing platform development utilizing the two aptamers.

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Section: Resultsmentioning

confidence: 99%

In-Solution Molecular Recognition Comparison of Aptamers against the Herbicide Atrazine

2019

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“…The electrochemical method was integrated with amplification strategies for assaying numerous targets, such as sulfadimethoxine, malathion, OTA, kanamycin, ampicillin, atrazine (ATZ), and acetamiprid [138,[203][204][205][206][207][208][209][210]. Sulfadimethoxine was assessed electrochemically with the assistance of nuclease amplification (Figure 11A), in which a gold surface of an electrode was immobilized with a DNA probe, and then hybridized with a specific aptamer for sulfadimethoxine, forming a dsDNA that inhibited their digestion via nuclease P1.…”

Section: Electrochemical Aptasensormentioning

confidence: 99%

“…Exo I was used to promote the autocatalytic cycling of malathion by enhancing the current change, with a linear response range of 0.5-600 ng/L [204]. OTA and ATZ were detected electrochemically and photoelectrochemically with the assistance of cycling amplification, with a linear range of 0.01 to 1 ng/mL and a LOD of 0.004 ng/mL for OTA [205] and linear range of 50 fM to 0.3 nM with LOD of 12.0 fM for ATZ [206], as outlined in Figure 11C,D.…”

Section: Electrochemical Aptasensormentioning

confidence: 99%

“…(C) OTA assay using TiO 2 nanotube electrode functionalized with DNA probe tagged with MB, reproduced from [205]. (D) Schematic protocols for photoelectrochemical aptasensor ATZ assay with nuclease signal amplification using TiO 2 nanotube covered by a layer of AuNPs, further functionalized with ATZ aptamer/graphene mixture as a sensor probe, reproduced from [206].…”

Section: Conclusion and Future Outlookmentioning

confidence: 99%

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Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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“…Profenofos and atrazine are applied in agricultural as anti-helminths and herbicides [3,4]. These environmental hormones will accumulate in livestock and crops with sewage and soil, nally enter the human body.…”

Section: Backgroudmentioning

confidence: 99%

Suspension array platform based on aptamer for high-throughput detection of five environmental hormones

Yao¹,

Jia²,

Ren³

et al. 2021

Preprint

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Background As a common small molecule substance, environmental hormones widely exist in nature, especially water sources, which have a profound effects in humans. Highly efficient and sensitive method for estrogens in the environment are essential. Results In this paper, a novel high-throughput platform was established based on five small hormones molecules specificity aptamer and magnetic beads (MBs). The results showed that the sensitivity of the proposed method are greatly improved. The limit of detection(LOD) of this method for atrazine(Atz), profenofos, bisphenolA(BPA), estradiol(E2), and polychlorinated biphenyls(PCBs) were 9.46, 20.75, 23.81, 8.97, 6.27 pg/mL, respectively. The Recovery rate of the diluted environmental hormones spiked in the samples of Haihe river were in the range of 87.5-111.02% with relative standard deviations (RSDs) lower than 28.44%. Conclusion This platform based on new complementary strand fragments can simultaneously rapid detection five environmental hormones. The whole procedure completed within 1.5h including sample treatment, incubation and detection, greatly improving the detection efficiency.

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Immobilization-free photoelectrochemical aptasensor for environmental pollutants: Design, fabrication and mechanism

Cited by 47 publications

References 48 publications

In-Solution Molecular Recognition Comparison of Aptamers against the Herbicide Atrazine

In-Solution Molecular Recognition Comparison of Aptamers against the Herbicide Atrazine

Recent Advances in Aptamer Sensors

Suspension array platform based on aptamer for high-throughput detection of five environmental hormones

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