Immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide for selective extraction of phosphoproteins

Novel Ti4+-Chelated Polyoxometalate/Polydopamine Composite Microspheres for Highly Selective Isolation and Enrichment of Phosphoproteins

“…In Figure d, both P 5 W 30 /PDA and P 5 W 30 /PDA-Ti 4+ contain C, N, O, and W, but Ti 2p peak only appears in P 5 W 30 /PDA-Ti 4+ , indicating that Ti 4+ is successfully immobilized on the surface of P 5 W 30 /PDA composites. In O 1s spectrum of P 5 W 30 /PDA-Ti 4+ (Figure S3a), the peak of 530.1 eV is ascribed to the W–O–W bond derived from P 5 W 30 ; the peak of 530.9 eV is ascribed to the Ti–O bond derived from Ti 4+ ; the peaks of 532.0 and 533.3 eV are ascribed to CO and C–OH bonds coming from PDA, respectively. As shown in Figure S3b, there is only one peak at 399.8 eV ascribed to C–NH–C in N 1s spectrum, indicating that the amine group is bound to the aromatic ring and an indole structure of PDA is formed .…”

“…Ti 4+ has a strong affinity toward phosphate groups, facilitating the adsorption of phosphoproteins on P 5 W 30 /PDA-Ti 4+ . Moreover, the P 5 W 30 framework containing PO 4 tetrahedron and WO 6 octahedron can form π-bond. , Therefore, there is π stacking interaction between the dp π-bonds of P 5 W 30 and PO 4 3– , further facilitating the selective capture of phosphoproteins. Ova has two dominant phosphorylated sites at serine-68 and/or 344, and the adsorption mechanism toward phosphoproteins is the same as β-ca.…”

Determination of Trace Phosphoprotein in Food Based on Fluorescent Probe-Triggered Target-Induced Quench by Electrochemiluminescence

“…To date, almost all of the investigations aimed at phosphosites in samples through the mass spectrometry (MS) method , and very few investigations of quantitative analysis and systematic comparison of the phosphoprotein level in different foods have been reported. This may be because the food sample matrix is too complicated and needs complex multistep enrichment procedures and expensive instruments.…”

Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment

“…These materials mainly include species based on coordination interactions and multiple hydrogen bonding. The coordination interaction-based materials take advantages of the strong affinity between phosphate groups and transition metal cations, representative with immobilized metal affinity chromatography (IMAC) materials [13][14][15] and TiO 2 [16,17]. For example, Zn 2+ -immobilized superparamagnetic nanoparticles with multivalent ligand molecules were developed and used for phosphoproteins enrichment [13].…”