Immobilization of Active Hydrogenases by Encapsulation in Polymeric Porous Gels

Elgren, Timothy E.; Zadvorny, Oleg A.; Brecht, E.; Douglas, Trevor; Зорин, Н. А.; Maroney, Michael J.; Peters, John W.

doi:10.1021/nl0512224

Cited by 21 publications

(19 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A convenient reductant for industrial use would be hydrogen, which can be used to regenerate NADH catalysed by hydrogenase enzymes, and which can be reformed from a portion of the methane fed to the system. Such a reaction might be achieved by co-immobilization of hydrogenase 52 with pMMO, which would have the further advantage that all the reactants are gas phase and all the products are aqueous, simplifying reactant–product separation. Other options include supplying the electrons electrochemically, or co-immobilizing pMMO with methanol dehydrogenase to yield formaldehyde as the product for collection.…”

Section: Discussionmentioning

confidence: 99%

Printable enzyme-embedded materials for methane to methanol conversion

et al. 2016

View full text Add to dashboard Cite

An industrial process for the selective activation of methane under mild conditions would be highly valuable for controlling emissions to the environment and for utilizing vast new sources of natural gas. The only selective catalysts for methane activation and conversion to methanol under mild conditions are methane monooxygenases (MMOs) found in methanotrophic bacteria; however, these enzymes are not amenable to standard enzyme immobilization approaches. Using particulate methane monooxygenase (pMMO), we create a biocatalytic polymer material that converts methane to methanol. We demonstrate embedding the material within a silicone lattice to create mechanically robust, gas-permeable membranes, and direct printing of micron-scale structures with controlled geometry. Remarkably, the enzymes retain up to 100% activity in the polymer construct. The printed enzyme-embedded polymer motif is highly flexible for future development and should be useful in a wide range of applications, especially those involving gas–liquid reactions.

show abstract

Section: Discussionmentioning

confidence: 99%

Printable enzyme-embedded materials for methane to methanol conversion

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Radical of TMEDA move from surface to far away to initiate the polymerization of PEG pre-polymer; (3) act as H donor: SO 4 -. or HOÁ produced by KPS rapidly diffused and attracted H atoms from DBK molecule far from the UV source, generating more free radicals (see Figure 6).…”

Section: Development Of High Efficient Photo-initiator Systemmentioning

confidence: 99%

“…2,3 Because of high cell density, rapid biochemical reaction and easy separation of solid from liquid, immobilized cells have great potentials in the application of wastewater treatment, chemical synthesis and biochemical reaction, etc. 4,5 However, most hydrogels were synthesized by thermal polymerization, in which toxic monomer, chemical crosslinker and initiator were involved, accompanying high temperature. 6 Furthermore, the polymerization always lasts for more than 4 or 5 h. 7 If cells or bacteria were present in the reaction mixture, the toxic reagents and high temperature would seriously harm their activities, long polymerization time worsening the situation.…”

Section: Introductionmentioning

confidence: 99%

The development of a highly efficient photo‐initiator system and its application in the photo‐immobilization of activated sludge

Qiao

Liu

2013

J of Applied Polymer Sci

View full text Add to dashboard Cite

In this study, a highly efficient photo-initiator system was developed, which contained potassium persulfate, N, N, N 0 , N 0 -tetramethylethylethylenediamine plus benzil dimethyl ketal. The photo-initiator system could successfully initiate poly (ethylene glycol) dimethyl-acrylate prepolymer to polymerize and crosslink under the irradiation of UV rays, in the presence of concentrated activated sludge, finally leading to the formation of immobilized activate sludge pellet beads. The presence of O 2 and thickness of the reaction solution did not influence the photo-immobilization process. Respiratory measurement result demonstrated that most activated sludge kept alive during the photo-immobilization. Mechanical strength of the immobilized cells could be improved by optimizing contents and ratio of the initiator system. The corresponding mechanism was also discussed. V C 2013 Wiley Periodicals, Inc. J. Appl.Polym. Sci. 2014, 131, 39838.

show abstract

“…Enzyme encapsulation in silica-derived sol-gel materials has been demonstrated to stabilize many enzymes. This procedure was applied to hydrogenase [97]. The majority of hydrogenase was shown to be entrapped in the gel and protected against proteolysis.…”

Section: Carbon Nanotubes For Biological Production Of Dihydrogenmentioning

confidence: 99%

Carbon Nanotube-Enzyme Biohybrids in a Green Hydrogen Economy

Poulpiquet¹,

Ciaccafava²,

Benomar³

et al. 2013

Syntheses and Applications of Carbon Nanotubes and Their Composites

View full text Add to dashboard Cite

Additional information is available at the end of the chapter http://dx.doi.org/10.5772/51782. Introduction "lternative energy pathways to replace depleting oil reserves and to limit the effects of global warming by reducing the atmospheric emissions of carbon dioxide are nowadays required. Dihydrogen appears as an attractive candidate because it represents the highest energy output relative to the molecular weight MJ kg -against MJ kg -for natural gas , and because its combustion delivers only water and heat. Whereas the main renewable sources of energy available in nature solar, wind, geothermal… need to be transformed, dihydrogen is able to transport and store energy. Dihydrogen can be produced from renewable energies, indirectly from photosynthesis via biomass transformation, or directly by bacteria. It can be converted into electricity using fuel cell technology. From all these properties and because it does not compete with food and water resources, dihydrogen has been defined as third generation biofuel. It thus emerges as a new fully friendly environmental energy vector. The use of dihydrogen as an energy carrier is not a new idea. Let us simply remember that Jules Verne, a famous French visionary novelist, wrote early in : I believe that O and H will be in the future our energy and heat sources [ ]. His prediction simply relied on the discovery a few years before of the fuel cell concept by C. Schönbein, then W. Groove, who demonstrated that when stopping water electrolysis, a current flow occurred in the reverse way [ ]. However in order to implement the dihydrogen economy and replace fossil fuels, there are significant technical challenges that need to be overcome in each of the following domains: . dihydrogen production and generation, . dihydrogen storage and transportation, . dihydrogen conversion to electrical energy."s opposed to widespread opinions, natural dihydrogen sources exist alone on the earth's surface. Local and continuous emanations of dihydrogen can be observed in cratonic zones, ophiolitic rocks or oceanic ridges [ ]. Dihydrogen is effectively produced in the upper mantle of the earth through natural oxidation of iron II -rich minerals, like ferromagnesians, by water of the hydrosphere. The ferrous iron is oxidized in ferric iron and water is concurrently reduced in dihydrogen, as given by following equation: Fe . Exploitation of these sources remains however difficult so far as dihydrogen does not accumulate on the earth subsurface, especially for two reasons. First because as a powerful energy source dihydrogen is quickly consumed biologically or abiotically , and second because as the lightest and most mobile gas it is not much retained by Earth's attraction and escapes in the atmosphere.Combined with water and hydrocarbons dihydrogen is nevertheless the most abundant element on earth. Green means to ecologically convert H containers into dihydrogen still remain however challenging. The energetic volume density of dihydrogen is low . MJ m -against MJ m -for natural gas so that sto...

show abstract

Immobilization of Active Hydrogenases by Encapsulation in Polymeric Porous Gels

Cited by 21 publications

References 9 publications

Printable enzyme-embedded materials for methane to methanol conversion

Printable enzyme-embedded materials for methane to methanol conversion

The development of a highly efficient photo‐initiator system and its application in the photo‐immobilization of activated sludge

Carbon Nanotube-Enzyme Biohybrids in a Green Hydrogen Economy

Contact Info

Product

Resources

About