Immobilization of Ag nanoparticles/FGF‐2 on a modified titanium implant surface and improved human gingival fibroblasts behavior

Abstract

The objective of this study was to form a rapid and firm soft tissue sealing around dental implants that resists bacterial invasion. We present a novel approach to modify Ti surface by immobilizing Ag nanoparticles/FGF-2 compound bioactive factors onto a titania nanotubular surface. The titanium samples were anodized to form vertically organized TiO(2) nanotube arrays and Ag nanoparticles were electrodeposited onto the nanotubular surface, on which FGF-2 was immobilized with repeated lyophilization. A uniform distribution of Ag nanoparticles/FGF-2 was observed on the TiO(2) nanotubular surface. The L929 cell line was used for cytotoxicity assessment. Human gingival fibroblasts (HGFs) were cultured on the modified surface for cytocompatibility determination. The Ag/FGF-2 immobilized samples displayed excellent cytocompatibility, negligible cytotoxicity, and enhanced HGF functions such as cell attachment, proliferation, and ECM-related gene expression. The Ag nanoparticles also exhibit some bioactivity. In conclusion, this modified TiO(2) nanotubular surface has a large potential for use in dental implant abutment.

Titania Nanotubes for Local Drug Delivery from Implant Surfaces

“…Upon cell study with human gingival fibroblasts (HGFs), the modified surfaces enhanced HGF functions with negligible cytotoxicity (Fig. 10.11b) [149]. In another strategy aiming towards enhancing osseointegration, Lee et al loaded N-acetyl cysteine (NAC) in TNTs and investigated its effect on bone formation in vitro and in vivo [150].…”

“…Studies with the human hepatocarcinoma [149], and c concept for applying TNTs on Ti wires as tiny implants for brain therapy [57]. Reproduced with permission from [57,149,152] cell line suggested increased cellular uptake of DNR and enhanced anti-tumor efficiency. Also shown was photocatalytic growth inhibition of cancer cells.…”

“…Loose TNT-like fragments/TiO 2 whiskers (with three-dimensional mesoporous structure) have also been demonstrated as nano-vehicles for carrying active anti-cancer drug daunorubicin (DNR) for anti-tumor functions [153]. Studies with the human hepatocarcinoma [149], and c concept for applying TNTs on Ti wires as tiny implants for brain therapy [57]. Reproduced with permission from [57,149,152] cell line suggested increased cellular uptake of DNR and enhanced anti-tumor efficiency.…”

“…In an attempt to prevent bacterial infection by quick but firm soft tissue seal formation around dental implants, TNTs on Ti surface was modified by loading Ag NPs and FGF-2 growth factor [149]. Upon cell study with human gingival fibroblasts (HGFs), the modified surfaces enhanced HGF functions with negligible cytotoxicity (Fig.…”

TiO₂ nanotube platforms for smart drug delivery: a review

“…HGFs were cultured on the Ag/FGF-2 immobilized TNTs for cytocompatibility determination. 119 The result indicated that Ag/FGF-2-decorated TNTs has an excellent cytocompatibility compared to pure Ti, and the immobilized FGF-2 could enhance HGF cell attachment and proliferation. Furthermore, Bhattarai et al examined the feasibility of chitosan-gold nanoparticles conjugated with plasmid DNA/c-myb (Ch-GNPs/c-myb)-coated Ti implants and inserted it into rat mandibles to determine its in vivo effect.…”