Immobilization of alkaline serine endopeptidase from Bacillus licheniformis on SBA-15 and MCF by surface covalent binding

Kannan, K.; Jasra, Raksh V.

doi:10.1016/j.molcatb.2008.04.007

Cited by 43 publications

(15 citation statements)

References 35 publications

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“…There was no significant peak observed for MCF. 30 After immobilization of PW 12 -APTES, the intensities of the reflections decrease, which could be assigned to the decrease in electron density contrast upon introduction of PW 12 -APTES into the mesoporous of the silica host materials. Figure 2b shows the XRD patterns of PW 12 /APTES@MCF (2 θ = 10-80 • ) .…”

Section: Oxidation Of the Benzylic Alcohols: General Proceduresmentioning

confidence: 98%

PW$_{12}$-APTES@MCF: effective nanosized mesoporous composites for the oxidation of benzyl alcohols

Fazaeli¹,

Aliyan²,

Foroushani³

et al. 2014

Turk J Chem

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Section: Oxidation Of the Benzylic Alcohols: General Proceduresmentioning

confidence: 98%

PW$_{12}$-APTES@MCF: effective nanosized mesoporous composites for the oxidation of benzyl alcohols

Fazaeli¹,

Aliyan²,

Foroushani³

et al. 2014

Turk J Chem

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“…Recently, the immobilization of enzymes onto mesoporous siliceous foams (MCFs) has been established . MCF is composed of a disordered pore system consisting of cage‐like pores (20–50 nm), which are cross‐linked by smaller windows (9–26 nm) .…”

Section: Introductionmentioning

confidence: 99%

“…Thus, MCF is an appropriate support material for the immobilization of larger enzymes or proteins. To improve the attractive interactions between the respective enzyme and the surface of the MCF, the latter can be modified by using differently substituted trialkoxy silanes …”

Section: Introductionmentioning

confidence: 99%

Immobilization of Alcohol Dehydrogenase from E. coli onto Mesoporous Silica for Application as a Cofactor Recycling System

Dreifke

Fröba

2017

ChemCatChem

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What intrigues you about the immobilization of enzymes? Enzymes are fascinating biocatalysts with ar emarkable three-dimensionals tructure. Prior to the immobilization of an enzyme,i ti sn ecessary to carefullyi nvestigate its response when applying different pH values, diverse ionic strengthso r variouss ubstrates. Herebyaconsistent and comprehensive view of the respective enzymei so btained progressively,w hich contributest ou nderstand the behavior of an immobilized enzyme.I ti si mpressive that an enzyme confined in nanopores retains its enzymatic activity. Why are mesoporouss ilicasp romising host materials for enzymes in general? The pore size of mesoporous silicas and their surface properties can be individually tailored according to the requirements of the respective enzyme. Hence, mesoporous silicas are versatile support materials. Furthermore, the syntheses of mesopo-rous silicas are well understood and are highly reproducible. Therefore, ac onstant quality of the support material can be guaranteed. Is the immobilizationo fe nzymes aseminal approach? Enzymes catalyze countless reactions in nature. Severalo f these reactions are used in laboratory as well as industrial scale. Additionally,n umerous enzymesa re knownt op ossess ab road substrate specificity,w hich enables them to convert substrates of non-natural origin too. Immobilization ensures re-usabilityo ft he enzyme. Thus, the high costs of an enzymatic catalytic route can be relativized and the advantageso f enzyme catalysis can be used to their full extent. The front cover artworkf or Issue 7/2017 is provided by M. Dreifke, F. J. Brieler and M. Frçba,U niversity of Hamburg. The image shows as ingle spherical cell of am esoporous cellular siliceous foam with an immobilized alcohol dehydrogenase (ADH).T he ADH is able to catalyze the oxidationo f2-propanol to acetone with concomitant reduction of NADP + + to NADPH. However,i nt he presence of as ufficient concentration of acetone, the back reactiona ccompanied by the oxidation of NADPH to NADP + + can be introduced too. See the Full Paper itself at http://dx.

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“…MCF is derived product of SBA-15 in which addition of swelling agents result in formation of 3D ultra cage spherical mesoporous structure connected by uniform window. The 3D MCF material has large surface area and smaller particle size, which is better for adsorbent and permits immobilization of greater amounts of enzyme [13][14][15]. Many diverse methodologies have been used by various groups of researchers including physical adsorption, covalent binding, encapsulation, entrapment, and crosslinking for immobilization of enzymes [10,12,16].…”

Section: Introductionmentioning

confidence: 99%

Kinetic resolution of (R,S)-α-tetralol catalyzed by crosslinked Candida antarctica lipase B enzyme supported on mesocellular foam: A nanoscale enzyme reactor approach

Kamble

Shinde

Yadav

2016

Journal of Molecular Catalysis B: Enzymatic

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Immobilization of alkaline serine endopeptidase from Bacillus licheniformis on SBA-15 and MCF by surface covalent binding

Cited by 43 publications

References 35 publications

PW$_{12}$-APTES@MCF: effective nanosized mesoporous composites for the oxidation of benzyl alcohols

PW$_{12}$-APTES@MCF: effective nanosized mesoporous composites for the oxidation of benzyl alcohols

Immobilization of Alcohol Dehydrogenase from E. coli onto Mesoporous Silica for Application as a Cofactor Recycling System

Kinetic resolution of (R,S)-α-tetralol catalyzed by crosslinked Candida antarctica lipase B enzyme supported on mesocellular foam: A nanoscale enzyme reactor approach

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