Immobilization of animal cells in porous carrier culture

Looby, D.; Griffiths, Bryan

doi:10.1016/0167-7799(90)90177-y

Cited by 66 publications

(18 citation statements)

References 4 publications

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“…However, to date, no such system is really used under industrial conditions. The main drawbacks are: (i) that the linear scale-up is limited due to concentration gradients, (ii) the system is nonhomogeneous, (iii) no sampling of the reactor content is possible, and (iv) the outgrowth of cells leads to channel blockage [82].…”

Section: Fixed Bed Reactor/packed Bed Reactormentioning

confidence: 99%

State-of-the-art of the production of retroviral vectors

Merten

2004

J. Gene Med.

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Retroviral vectors are still the vectors that are used in the majority of gene therapy trials for treatment of acquired or inherited diseases. In this review, the present state-of-the-art of the production of retroviral vectors and the most important parameters, such as the choice of the producer cell line, stability issues, medium additives, serum, type of bioreactor, that influence production issues is presented and discussed in light of an optimal vector production. The available literature data clearly indicate that, on one hand, the choice of the producer cell line is of utmost importance for obtaining a high level producer cell line, and that, on the other hand, the optimization of the medium, e.g. the replacement of glucose by fructose, has a potential for improving vector production rates and titers. Finally, the use of high-density perfusion culture systems for adherent as well as for suspension cells presents the best choice for a production system, because high cell densities imply high reactor specific production rates, which must be associated with a rapid harvest of the produced vector, thus avoiding vector inactivation due to an extended residence time. The overall optimization of the cultivation and production parameters will have a significant impact on the use of retroviral vectors for gene therapy purposes.

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Section: Fixed Bed Reactor/packed Bed Reactormentioning

confidence: 99%

State-of-the-art of the production of retroviral vectors

Merten

2004

J. Gene Med.

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“…The successful application of an immobilised cell system as a biocatalyst relies on the correct choice of the main components of the system, namely the matrix material, the cell type and the configuration of the immobilization system. Among the desirable characteristics for immobilized cell systems are a high surface area-to-volume ratio, chemical and mechanical stability and optimum diffusion of oxygen and nutrients [41]. However, the use of bioreactors specifically for immobilized cells is still in its infancy.…”

Section: Immobilized Cell Bioreactorsmentioning

confidence: 99%

Bioreactors

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Anthony

2010

Plant Cell Culture

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“…The largest practical packed-bed volume in the configuration of the Celligen Plus reactor (Figure 2a) is approximately 20 l, providing 200-300 l of supernatant (Merten 2004). No industrial processes currently employ packed-bed bioreactors as they have limited linear scale-up due to concentration gradients, the system is non-homogeneous, it is not possible to sample reactor content and the outgrowth of cells leads to channel blockage (Looby and Griffiths 1990). The disadvantage of the CellCube fixed bed bioreactor is that some components, such as the oxygenator and the probes, must be cleaned and re-sterilized between batches.…”

Section: Bioreactor Designmentioning

confidence: 99%

Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes

2006

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Gene therapy is a promising technology for the treatment of several acquired and inherited diseases. However, for gene therapy to be a commercial and clinical success, scalable cell culture processes must be in place to produce the required amount of viral vectors to meet market demand. Each type of vector has its own distinct characteristics and consequently its own challenges for production. This article reviews the current technology that has been developed for the efficient, large-scale manufacture of retrovirus, lentivirus, adenovirus, adeno-associated virus and herpes simplex virus vectors.

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Immobilization of animal cells in porous carrier culture

Cited by 66 publications

References 4 publications

State-of-the-art of the production of retroviral vectors

State-of-the-art of the production of retroviral vectors

Bioreactors

Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes

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