Immobilization of antibodies in micropatterns for cell detection by optical diffraction

Morhard, F; Pipper, J.; Dahint, R.; Grunze, M.

doi:10.1016/s0925-4005(00)00574-8

Cited by 65 publications

(45 citation statements)

References 12 publications

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“…This device, with sensitivity up to 1 pg/ml antigen in human saliva, opened a new perspective in the monitoring and control of disease spreading [187]. An alternative approach aimed to disease control was provided by the pioneering work of Morhard et al in year 2000, which reported bacterial detection by antibodies patterned by μCP [188]. Escherichia coli antibodies were directly imprinted on a rigid substrate.…”

Section: Sensingmentioning

confidence: 99%

Nanoimprint Lithography: Toward Functional Photonic Crystals

Lova

Soci

2015

Organic and Hybrid Photonic Crystals

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In this chapter we review the use of nanoimprint lithography and its derivative soft-lithography techniques for the fabrication of functional photonic crystals. Nanoimprint is a viable, scalable, and cost-effective solution for large area patterning. While initially it relied primarily on pattern transfer from a rigid mold to a thermally softened polymer by embossing, in the last two decades the process evolved rapidly, giving rise to new technologies that allow direct imprint of functional materials such as conjugated polymers, metals, biological matter, and metal oxides. These advancements generated increasing interest in the use of nanoimprint lithography for the fabrication of photonic structures for light management in optoelectronic devices. After describing standard nanoimprint lithography and its derivative soft-lithography methods, we briefly discuss nanoimprint capabilities and prospects in photonic applications. In particular we review recent implementations of imprinted photonic structures for light management in organic light emitting diodes, solar cells, solid state lasers and sensors.

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Section: Sensingmentioning

confidence: 99%

Nanoimprint Lithography: Toward Functional Photonic Crystals

Lova

Soci

2015

Organic and Hybrid Photonic Crystals

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“…An extensive study of surface modifications and different stamping procedures as well as several printed patterns is given in Ref. [36] After trying a wide variety of immobilization protocols the direct microcontact printing of suitable derivatized antibodies gave the best results. This method ensures stable, homogeneous covering of the transducer surface hence is ideal for binding microorganisms.…”

Section: Optical Sensorsmentioning

confidence: 99%

Sensor strategies for microorganism detection?from physical principles to imprinting procedures

Dickert

Lieberzeit

Hayden

2003

Analytical and Bioanalytical Chemistry

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“…For example, by the diffraction patterns generated by a grating structure formed by the Escherichia coli bacteria, Morhard et al (2000) could detect their concentrations of higher than 10 6 cells/ ml. In a similar way, Williams et al (1991) detected the growth of yeast and bacteria, and St.…”

Section: Introductionmentioning

confidence: 99%

“…Different from all of the above literature reports, this paper for the first time utilizes merely the diffraction of an optical aperture or an aperture array to detect the biological cells. It does not need the cells to be organized in a periodic pattern (Morhard et al 2000;Williams et al 1991;St. John et al 1998), does not use diffraction gratings (Chen et al 2005), and its aperture works as an imaging formation element instead of amplifying a lens formed image (King et al 2005), and most of all, it is integrated in a robust and inexpensive MEMS chip.…”

Section: Introductionmentioning

confidence: 99%

Design of MEMS devices with optical apertures for the detection of transparent biological cells

Zhou

Poenar

Liu

et al. 2008

Biomed Microdevices

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This paper provides a novel technique to detect transparent biological living cells trapped in a microfluidic MEMS device by optical diffraction. The device essentially consists of an optical aperture or an aperture array patterned in metal layer and a microfluidic chamber positioned above the center of the aperture. When the cells in the chamber are illuminated through the aperture, the far-field diffraction pattern can be recorded by a CCD camera or a photodetector array. This diffraction pattern uniquely corresponds to the sizes, positions, and intrinsic optical properties of the aperture, cells, and the microfluidic chamber materials, so any unknown but relevant parameter is able to be extrapolated when all other parameters are fixed or identified. This paper describes in detail the designs of various microfluidic chambers and apertures for this application, and the development of a complete set of software for the analysis of the cells' optical properties. Compared with other currently available methods for the detection of transparent living cells, this method has the advantages of simple device structure, easy to manipulate, able to simultaneously detect several cells of different species, as well as providing accurate and sensitive results. Besides the detection of living cells, this technique can also be used to detect or characterize other transparent or low optical absorption particles, such as polymer spheres or insoluble droplets, inside an aqueous solution.

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Immobilization of antibodies in micropatterns for cell detection by optical diffraction

Cited by 65 publications

References 12 publications

Nanoimprint Lithography: Toward Functional Photonic Crystals

Nanoimprint Lithography: Toward Functional Photonic Crystals

Sensor strategies for microorganism detection?from physical principles to imprinting procedures

Design of MEMS devices with optical apertures for the detection of transparent biological cells

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