Immobilization of antibodies on mesoporous silica materials and their binding abilities to antigens

Orita, Toru; Kato, Katsuya; Tomita, Masahiro

doi:10.2109/jcersj2.119.238

Cited by 17 publications

(8 citation statements)

References 32 publications

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“…These observations are consistent with earlier reports regarding the encapsulation of enzymes and antibodies in MSNPs. 50,75,76 ■. CONCLUSIONS We described the preparation of a new type of templated mesoporous silica nanoparticles (TA-MSNPs) with unique pore morphology, large pore volume, and narrow pore size distribution.…”

Section: Chemistry Of Materialsmentioning

confidence: 99%

Large Pore Mesoporous Silica Nanoparticles by Templating with a Nonsurfactant Molecule, Tannic Acid

2014

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We describe a one-pot synthesis of novel large-pore mesoporous silica nanoparticles using a nonsurfactant template, tannic acid, and protein immobilization on these particles. The tannic-acid-templated mesoporous silica nanoparticles (TA-MSNPs) possess tunable large pores (6−13 nm), unique pore morphology, and a uniform diameter of ca. 200 nm. TA-MSNPs show high protein adsorption capacity of 77.1 mg/g for lysozyme, 396.5 mg/g for bovine hemoglobin, and 130.0 mg/g for bovine serum albumin and rapid protein uptake. They can also adsorb a large amount of m-MDH (421 ± 13 mg/ g) and protect this enzyme from the environment, as demonstrated by its high activity when encapsulated inside the mesopores of TA-MSNPs. ■. INTRODUCTIONMesoporous silica nanoparticles (MSNPs) attract increasing attention because of their high surface area, hydrophilic and easily functionalized surface, and biocompatibility. These properties are of high interest for a host of applications, 1,2 including drug delivery, 3 imaging, 4−6 catalysis, 7,8 and sensing. 9 To fulfill this promise, especially in the biomedical area, monodisperse MSNPs with sizes in the 50−200 nm range (to facilitate cell uptake 10,11 ) and with large mesopores (to allow for the encapsulation of biomacromolecules) are desired. In addition, accessible internal volume resulting from interconnected pores would be beneficial for these applications. 12,13 Numerous strategies for the preparation of MSNPs have been developed 14 using templating agents, usually surfactants. 15 Ionic surfactants, such as cetyltrimethylammonium bromide (CTAB), self-assemble into micelles, which create the voids in the silica matrix during the preparation of mesoporous silica materials, such as MCM-41. The formation of the mesoporous structure of MCM-41 is mainly due to the electrostatic interactions between the ionic surfactant and silicate species. 15 For nonionic surfactants, such as triblock copolymers, the hydrogen bonding between the surfactants and the silicate is believed to be the main driving force in the formation of mesoporous silica materials, such as SBA-15. 15 Fluorocarbon surfactants were also used to synthesize mesoporous silica with improved stability and novel mesostructures. 16 In all these cases, templating surfactant agents lead to ordered structures with noninterconnected cylindrical pores. 15 Several synthetic protocols for MSNPs with particle diameter and pore size controlled by using various surfactants and swelling agents have been reported. 12−19 For example, MCM-41 nanoparticles can be prepared with diameters in the 30−280 nm range; 20 however, the maximum pore size is limited to ca. 6 nm. SBA-15 nanoparticles 21 contain mesopores of larger sizes, controlled by triblock-copolymer templates, cosolvents, and swelling agents in the range of 6 to 50 nm. However, the size of the smallest SBA-15 particles reported so far is around 500 nm. 5,22 It remains a challenge to produce uniform MSNPs with diameters smaller than 200 nm and pore size larger than 10 nm in a simple synthetic p...

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Section: Chemistry Of Materialsmentioning

confidence: 99%

Large Pore Mesoporous Silica Nanoparticles by Templating with a Nonsurfactant Molecule, Tannic Acid

2014

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“…The unique features, such as high surface area and large pore volume, make mesoporous silica an excellent candidate to be an immobilizing carrier for immunoassays. 7) Mesoporous silica is a potential material for the adsorption of antibodies. Endo et al reported formation of a thick coating of mesoporous silica powder on stainless steel by an electrophoretic deposition method.…”

Section: Mesoporous Materials By Self-assembly Of Surfactantsmentioning

confidence: 99%

Field-Induced alignment controls of one-dimensional mesochannels in mesoporous materials

Yamauchi

2013

J. Ceram. Soc. Japan

147

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Mesoporous materials prepared through the self-assembly of surfactants have attracted wide attention because of their many potential applications. The mesostructural, compositional, and morphological controls of mesoporous materials have been extensively studied thus far. In particular, the macroscopic alignments of one-dimensional (1D) mesochannels and their controls are quite important for the creation of advanced functional materials. In most cases, the direction of mesochannels in mesoporous films lies parallel to the substrate. Vertical orientation of the mesochannels can realize high diffusion and accessibility of guest species from the outside. Here, I review the recent progress on this emerging research field. Various methods have been proposed for the preparation of vertically oriented mesoporous thin films using high magnetic field, shear flow, modification of the substrate, and other methods. I also briefly introduce their applications and a perspective for the future.©2013 The Ceramic Society of Japan. All rights reserved.Key-words : Mesoporous material, Surfactant, Thin film, High magnetic processing [Received April 25, 2013; Accepted June 23, 2013] 1. Mesoporous materials by self-assembly of surfactants Ordered mesoporous materials prepared through 'self-assembly of surfactants' have attracted growing interest due to their special properties, including uniform mesopores (250 nm) and high specific surface areas. 1),2) According to the International Union of Pure and Applied Chemistry (IUPAC), porous materials are classified according to their pore sizes: microporous materials with pore sizes below 2 nm, mesoporous materials with pore sizes between 2 and 50 nm, and macroporous materials with pore sizes exceeding 50 nm.3),4) Zeolites are traditional microporous materials that have been utilized for practical applications in catalysts and adsorbents. Although the microporous materials have high surface areas, due to their small-sized pores, their applications are limited to reactions only for relatively small molecules. Recently, metalorganic frameworks (MOFs) or porous coordination polymers (PCPs) consisting of metal ions coordinated with organic molecules have been extensively studied and used for the storage of gases such as hydrogen and carbon dioxide. 5),6) However, their pore size is still small. In contrast, several types of macroporous materials, such as inverse opal films, track-etched polycarbonate membranes, and porous anodic alumina membranes, have been reported thus far. However, such pore expansion seriously decreases surface area.Unlike other porous materials, mesoporous materials can be easily prepared under a wide range of synthetic conditions. Mesoporous materials exhibit several defined mesostructures, such as lamellar, two-dimensional (2D) hexagonal, three-dimensional (3D) hexagonal, and bicontinuous cubic mesostructures. Furthermore, many framework compositions (e.g., silica, organosilica, metal, and non-siliceous oxides) and various morphologies (e.g., nanoparticle, monolith, a...

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“…Since enzymes are inactivated owing to structural changes triggered by solvent influences, prevention of conformational change in the limited space results in retention of activity. 22 When the size is matched between enzymes and nano-space, this effect is more pronounced. In case of MC, the size of FDH and pore diameter matches very well.…”

Section: Stability Of Fdh Against the Outer Environmentmentioning

confidence: 99%

Immobilization of an Enzyme Into Nano-Space of Nanostructured Carbon and Evaluation as Electrochemical Sensors

Hayashi¹,

Kato

Sasaki

2015

j nanosci nanotechnol

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The aims of this study are immobilization of formaldehyde dehydrogenase (FDH) into nano-space of nanostructured carbon and evaluation as a possible sensor detecting low concentration formaldehyde. In order to understand the effect of carbon pore size on activity and stability of FDH, mesoporous carbon (MC), originally made in our lab, and commercially available ketjen black (KB) were used in this study. Enzyme activity and electrochemical sensing ability of FDH encapsulated into such two carbon materials were compared. Our original MC resulted in favourable in some evaluated conditions but not in other conditions. MC adsorbed FDH less than KB, but enzyme activity was higher on MC per FDH. Stability against methanol increased on MC, but stability against water was rather lower on MC. Electrochemical sensing ability toward formaldehyde resulted in much better on KB, which was able to detect the sub-ppb level of formaldehyde. Consequently, such dependence is resulted by available nano-space of carbon, and so tuning the pore size of carbon is an important factor in order to develop enzyme based electrochemical sensors with high sensitivity and stability.

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Immobilization of antibodies on mesoporous silica materials and their binding abilities to antigens

Cited by 17 publications

References 32 publications

Large Pore Mesoporous Silica Nanoparticles by Templating with a Nonsurfactant Molecule, Tannic Acid

Large Pore Mesoporous Silica Nanoparticles by Templating with a Nonsurfactant Molecule, Tannic Acid

Field-Induced alignment controls of one-dimensional mesochannels in mesoporous materials

Immobilization of an Enzyme Into Nano-Space of Nanostructured Carbon and Evaluation as Electrochemical Sensors

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