Immobilization of antimicrobial and anti-quorum sensing enzymes onto GMA-grafted poly(vinyl chloride) catheters

Costoya, Alejandro; Becerra, Luis E. Velázquez; Meléndez‐Ortiz, H. Iván; Díaz-Gómez, Luis; Mayer, Celia; Otero, Ana; Concheiro, Angel; Bucio, Emilio; Alvarez‐Lorenzo, Carmen

doi:10.1016/j.ijpharm.2018.12.075

Cited by 24 publications

(24 citation statements)

References 62 publications

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“…An increase of the grafting yield with respect to the concentration and the irradiation gamma dose was observed, achieving values up to 200%. Costoya et al [ 40 ] grafted glycidyl methacrylate (GMA) onto poly(vinyl chloride) (PVC). A pre-irradiation dose of 40 kGy produced a yield of 120%; whereas, Caner et al [ 41 ] chemically grafted poly(N-vinylimidazole) onto chitosan via ceric ion initiation, achieving 140% of grafting yield.…”

Section: Resultsmentioning

confidence: 99%

Obtainment and Characterization of Hydrophilic Polysulfone Membranes by N-Vinylimidazole Grafting Induced by Gamma Irradiation

et al. 2020

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Polysulfone (PSU) film and N-vinylimidazole (VIM) were used to obtain grafted membranes with high hydrophilic capacity. The grafting process was performed by gamma irradiation under two experiments: (1) different irradiation doses (100–400 kGy) and VIM 50% solution; (2) different concentration of grafted VIM (30–70%) and 300 kGy of irradiation dose. Characteristics of the grafted membranes were determined by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), contact angle, swelling degree, desalination test, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Both experiments indicated that the absorbed dose 300 kGy and the VIM concentration, at 50% v/v, were effective to obtain PSU grafted membranes with 14.3% of grafting yield. Nevertheless, experimental conditions, 400 kGy, VIM 50% and 300 kGy, VIM 60–70% promoted possible membrane degradation and VIM homopolymerization on the membrane surface, which was observed by SEM images; meanwhile, 100–200 kGy and VIM 30–50% produced minimal grafting (2 ± 0.5%). Hydrophilic surface of the grafted PSU membranes by 300 kGy and VIM 50% v/v were corroborated by the water contact angle, swelling degree and desalination test, showing a decrease from 90.7° ± 0.3 (PSU film) to 64.3° ± 0.5; an increment of swelling degree of 25 ± 1%, and a rejection-permeation capacity of 75 ± 2%. In addition, the thermal behavior of grafted PSU membranes registered an increment in the degradation of 20%, due to the presence of VIM. However, the normal temperature of the membrane operation did not affect this result; meanwhile, the glass transition temperature (Tg) of the grafted PSU membrane was found at 185.4 ± 0.5 °C, which indicated an increment of 15 ± 1%.

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Section: Resultsmentioning

confidence: 99%

Obtainment and Characterization of Hydrophilic Polysulfone Membranes by N-Vinylimidazole Grafting Induced by Gamma Irradiation

et al. 2020

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“…The modified SR films were dried under a vacuum at 60 °C. Finally, the graft percentage of the films was calculated was using the following gravimetry equation (Equation (1)) [ 12 ]:

where Wo and W represent the mass of the SR film before and after grafting, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The samples were irradiated with a 60 Co source in the dose interval from 20 to 100 kGy, and the monomer concentration ranged from 0.4 to 1.5 M. The films were washed in ethanol to remove the unrealized graft of poly(2MBA) or homopolymers. The modified SR films were dried under a vacuum at 60 • C. Finally, the graft percentage of the films was calculated was using the following gravimetry equation (Equation 1) [12]:…”

Section: Grafting Process (Sr-g-2mba)mentioning

confidence: 99%

“…Gamma radiation (specifically 60 Co) is a technique for medical device modification, and it is used to graft or functionalize polymers on the surface of a device and to achieve drug and enzyme loading/release [12,13]. The method is based on the irradiation of the materials with gamma rays, which generates radicals on the surface that initiate the grafting polymerization process (the direct method) [14].…”

Section: Introductionmentioning

confidence: 99%

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Radiation Grafting of a Polymeric Prodrug onto Silicone Rubber for Potential Medical/Surgical Procedures

et al. 2020

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Silicone rubber (SR) is a material used for medical procedures, with a common example of its application being in implants for cosmetic or plastic surgeries. It is also an essential component for the development of medical devices. SR was functionalized with the polymeric prodrug of poly(2-methacryloyloxy-benzoic acid) (poly(2MBA)) to render the analgesic anti-inflammatory drug salicylic acid by hydrolysis. The system was designed by functionalizing SR films (0.5 cm × 1 cm) with a direct grafting method, using gamma irradiation (60Co source) to induce the polymerization process. The absorbed dose (from 20 to 100 kGy) and the monomer concentration (between 0.4 and 1.5 M) were critical in controlling the surface and the bulk modifications of SR. Grafting poly(2MBA) onto SR (SR-g-2MBA) were characterized by attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy/energy-dispersive X-ray spectrometry, fluorescence microscopy, the contact angle, and the swelling. SR-g-2MBA demonstrated the drug’s sustained and pH-dependent release in simulated physiological mediums (pH = 5.5 and 7.4). The drug’s release was quantified by high-performance liquid chromatography and confirmed by gas chromatography–mass spectrometry. Finally, cytocompatibility was demonstrated in murine fibroblast and human cervical cancer cell lines. The developed systems provide new polymeric drug release systems for medical silicone applications.

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“…Polyvinylchloride [25] The bag containing blood and solutions Polyethylene [26] Bone related implants Nowadays, polymer-based implantable devices like bioengineered veins, plates of bone, dental parts, muscles, discs of vertebral, valves of heart, and pacemakers are used in biomedical engineering to reinstate the damaged organs [5,6]. Polymer-based biomaterials can provide assistance for the healing process in the human body.…”

Section: Biomaterialsmentioning

confidence: 99%

Fabrication and Analysis of Polydimethylsiloxane (PDMS) Microchannels for Biomedical Application

et al. 2020

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In this research work, Polydimethylsiloxane (PDMS) has been used for the fabrication of microchannels for biomedical application. Under the internet of things (IoT)-based controlled environment, the authors have simulated and fabricated bio-endurable, biocompatible and bioengineered PDMS-based microchannels for varicose veins implantation exclusively to avoid tissue damaging. Five curved ascending curvilinear micro-channel (5CACMC) and five curved descending curvilinear micro-channels (5CDCMC) are simulated by MATLAB (The Math-Works, Natick, MA, USA) and ANSYS (ANSYS, The University of Lahore, Pakistan) with actual environments and confirmed experimentally. The total length of each channel is 1.6 cm. The diameter of both channels is 400 µm. In the ascending channel, the first to fifth curve cycles have the radii of 2.5 mm, 5 mm, 7.5 mm, 10 mm, and 2.5 mm respectively. In the descending channel, the first and second curve cycles have the radii of 12.5 mm and 10 mm respectively. The third to fifth cycles have the radii of 7.5 mm, 5 mm, and 2.5 mm respectively. For 5CACMC, at Reynolds number of 185, the values of the flow rates, velocities and pressure drops are 19.7 µLs−1, 0.105 mm/s and 1.18 Pa for Fuzzy simulation, 19.3 µLs−1, 0.1543 mm/s and 1.6 Pa for ANSYS simulation and 18.23 µLs−1, 0.1332 mm/s and 1.5 Pa in the experiment. For 5CDCMC, at Reynolds number 143, the values of the flow rates, velocities and pressure drops are 15.4 µLs−1, 0.1032 mm/s and 1.15 Pa for Fuzzy simulation, 15.0 µLs−1, 0.120 mm/s and 1.22 Pa for ANSYS simulation and 14.08 µLs−1, 0.105 mm/s and 1.18 Pa in the experiment. Both channels have three inputs and one output. In order to observe Dean Flow, Dean numbers are also calculated. Therefore, both PDMS channels can be implanted in place of varicose veins to have natural blood flow.

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Immobilization of antimicrobial and anti-quorum sensing enzymes onto GMA-grafted poly(vinyl chloride) catheters

Cited by 24 publications

References 62 publications

Obtainment and Characterization of Hydrophilic Polysulfone Membranes by N-Vinylimidazole Grafting Induced by Gamma Irradiation

Obtainment and Characterization of Hydrophilic Polysulfone Membranes by N-Vinylimidazole Grafting Induced by Gamma Irradiation

Radiation Grafting of a Polymeric Prodrug onto Silicone Rubber for Potential Medical/Surgical Procedures

Fabrication and Analysis of Polydimethylsiloxane (PDMS) Microchannels for Biomedical Application

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