Immobilization of bilirubin oxidase on graphene oxide flakes with different negative charge density for oxygen reduction. The effect of GO charge density on enzyme coverage, electron transfer rate and current density

Filip, Jaroslav; Andicsovà‐Eckstein, Anita; Vikartovská, Alica; Tkac, Jan

doi:10.1016/j.bios.2016.06.006

Cited by 34 publications

(16 citation statements)

References 49 publications

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“…While HRP and laccase have been vital in enzyme biosensor studies, other enzymes can be immobilized to create highly specific biosensors. For example, bilirubin oxidase was immobilized on GO-based surfaces [ 115 , 116 ]. Such biosensors can have a significant impact in the medical field due to their ability to detect bilirubin, an essential compound for assessing liver function.…”

Section: Graphene-based Nanomaterials and Enzymesmentioning

confidence: 99%

Recent advances in graphene-based biosensor technology with applications in life sciences

et al. 2018

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Graphene’s unique physical structure, as well as its chemical and electrical properties, make it ideal for use in sensor technologies. In the past years, novel sensing platforms have been proposed with pristine and modified graphene with nanoparticles and polymers. Several of these platforms were used to immobilize biomolecules, such as antibodies, DNA, and enzymes to create highly sensitive and selective biosensors. Strategies to attach these biomolecules onto the surface of graphene have been employed based on its chemical composition. These methods include covalent bonding, such as the coupling of the biomolecules via the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide reactions, and physisorption. In the literature, several detection methods are employed; however, the most common is electrochemical. The main reason for researchers to use this detection approach is because this method is simple, rapid and presents good sensitivity. These biosensors can be particularly useful in life sciences and medicine since in clinical practice, biosensors with high sensitivity and specificity can significantly enhance patient care, early diagnosis of diseases and pathogen detection. In this review, we will present the research conducted with antibodies, DNA molecules and, enzymes to develop biosensors that use graphene and its derivatives as scaffolds to produce effective biosensors able to detect and identify a variety of diseases, pathogens, and biomolecules linked to diseases.

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Section: Graphene-based Nanomaterials and Enzymesmentioning

confidence: 99%

Recent advances in graphene-based biosensor technology with applications in life sciences

et al. 2018

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“…It can be seen that high value (10 A/g·cm -2 for rGO-10min) was obtained in this work suggesting that O 2 plasma processing of rGO can be sufficient for tailoring properties of rGO surface for BOD adsorption. Cosnier et al [46] Small-flakes rGO BOD 18 Tkac et al [47] Oxygen-plasma treated rGO BOD 10 This work…”

Section: Effect Of Treatment Time On Degree Of Surface Functionalizationmentioning

confidence: 92%

Tailoring properties of reduced graphene oxide by oxygen plasma treatment

Kondratowicz

Nadolska

Şahin

et al. 2018

Applied Surface Science

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“…MET EBFCs rely heavily on redox mediators to shuttle electrons between biocatalytic active sites and electrode surfaces, whereas DET EBFCs enable electron transfer from the enzyme active sites directly to the electrode . In either case, the other electrode (cathode) requires an oxygen‐reduction catalyst, such as laccase or bilirubin oxide (BOD), to facilitate the oxygen reduction reaction (ORR). Unfortunately, in general, the current catalysts under investigation show very low catalytic performance as well as poor adhesion to the electrode surface.…”

Section: Figurementioning

confidence: 99%

In Situ Engineering of Intracellular Hemoglobin for Implantable High‐Performance Biofuel Cells

et al. 2019

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The key challenge for the broad application of implantable biofuel cells (BFCs) is to achieve inorganicorganic composite biocompatibility while improving the activity and selectivity of the catalysts.W eh ave fabricated nanoengineered red blood cells (NERBCs) by an environmentally friendly method by using red blood cells as the raw material and hemoglobin (Hb) embedded with ultrasmall hydroxyapatite (HAP,C a 10 (PO 4 ) 6 (OH) 2 )a st he functional BFC cathode material. The NERBCs showed greatly enhanced cell performance with high electrocatalytic activity,s tability, and selectivity.T he NERBCs maintained the original biological properties of the natural cell, while enhancing the catalytic oxygen reduction reaction (ORR) through the interaction between À OH groups in HAP and the Hb in RBCs.They also enabled direct electron transportation, eliminating the need for an electron-transfer mediator,a nd showed catalytic inactivity for glucose oxidation, thus potentially enabling the development of separator-free BFCs.Enzyme biofuel cells (EBFCs) [1] have attracted considerable attention as micro- [2] or even nanoscale power [3] sources for implantable biomedical devices,s uch as cardiac pacemakers, [4] implantable self-powered sensors, [5] and biosensors for monitoring physiological parameters. [6] There are two types of EBFCs,w hich differ in the operating mechanism;n amely, mediated electron transfer (MET) [7] and direct electron transfer (DET) [8] EBFCs.M ET EBFCs rely heavily on redox mediators to shuttle electrons between biocatalytic active sites and electrode surfaces,w hereas DET EBFCs enable electron transfer from the enzyme active sites directly to the electrode. [9] In either case,t he other electrode (cathode) requires an oxygen-reduction catalyst, such as laccase [10] or bilirubin oxide (BOD), [11] to facilitate the oxygen reduction reaction (ORR). Unfortunately,i ng eneral, the current catalysts under investigation show very low catalytic performance as well as poor adhesion to the electrode surface. Such drawbacks drastically limit the practical application of EBFCs. [12] Although approaches such as electrode nanomodification, [1b] enzyme immobilization, [13] and redox-mediator addition [14] have been intensively investigated to facilitate electron transfer between enzymes and electrodes,t hey usually face some challenges,s uch as avoiding the deformation and inactivation of enzymes, [15] preventing nanotoxicity, [16] or improving the stability of the cell. [17] As is well-known, the biosafety of nanomaterials synthesized in vitro for long-term operation in the body,i no ther words,t he use of invasive, external, and foreign (relative to the nature of the cell) nanomaterials,i sc ontroversial, for many reasons,i ncluding their unexpected migration and accumulation. [18] Nanobioengineering, [19] which has already seen great success in the fields of medicine,agriculture,environment, and electronic systems offers ap otential opportunity to tackle these challenges.I n situ biomineralization [20] processes...

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Immobilization of bilirubin oxidase on graphene oxide flakes with different negative charge density for oxygen reduction. The effect of GO charge density on enzyme coverage, electron transfer rate and current density

Cited by 34 publications

References 49 publications

Recent advances in graphene-based biosensor technology with applications in life sciences

Recent advances in graphene-based biosensor technology with applications in life sciences

Tailoring properties of reduced graphene oxide by oxygen plasma treatment

In Situ Engineering of Intracellular Hemoglobin for Implantable High‐Performance Biofuel Cells

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