Immobilization of bioluminescent Escherichia coli cells using natural and artificial fibers treated with polyethyleneimine

Chu, Yi; Hsu, Chia-Hua; Soma, Pavan Kumar; Lo, Y. Martin

doi:10.1016/j.biortech.2009.01.072

Cited by 51 publications

(33 citation statements)

References 41 publications

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“…It was found that the isomaltulose production increased because of the high cell retention and the high physical strength of the treated beads during fermentation compared to the non-treated beads. Moreover, Chu et al 12 reported that viscose fiber treated with 0.667% PEI was found capable of immobilizing the most viable recombinant E. coli. The PEI is a highly branched cationic polymer and has been widely used as a crosslinker to enhance the attachment of the cell to the matrix 27 .…”

Section: Resultsmentioning

confidence: 99%

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Effect of the cross linkers’ concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase)

CheMan

Illias

Sulaiman

et al. 2017

Chem. Eng. Res. Bull.

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Abstract:The excretion of recombinant proteins into the culture medium is a preferred approach over cytoplasmic expression because of the high specific activity and ease of purification. However, cell lysis is one of a major problem during the excretion of recombinant protein due to the pressure build up in the periplasmic space through overproduction of the expressed recombinant protein. Cell immobilization is a promising solution for the improvement of protein excretion with reduction in cell lysis. In this study, the effect of cross linkers concentrations on the process of cell immobilization for the cyclodextringlucanotransferase (CGTase) excretion and cell viability were investigated. The hollow fiber membrane was treated using different concentrations (0.3, 0.67 and 1%) of cross linkers namely glutaraldehyde (GA), poly-L-lysine (PLL) and polyethylenimine (PEI). The optimum concentration of cross linkers was found to be 0.3% with the high excretion of CGTase. Interestingly, the immobilized cell on the non-treated hollow fiber membrane showed up to 15% increment of CGTase excretion with 55% reduction of cell lysis, as compared to the immobilized cells on the treated hollow fiber membrane. It could be concluded that, the low concentration of cross linkers exhibited the highest CGTase excretion. Moreover, the non-treated hollow fiber membrane is a promising approach for attachment of cells to the membrane without requiring treatment with any chemicals for the production of recombinant enzyme.

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Section: Resultsmentioning

confidence: 99%

“…The effect of crosslinkers[glutaraldehyde (GA), poly-L-lysine (PLL) and polyethyleneimine (PEI)] concentration on CGTase excretion and cell viability of the immobilized cell was studied using concentrations of 0.30, 0.67 and 1.00 % based on the study conducted by Chu et al 12 . The hollow fiber membranes were rinsed with the sterile distilled water and dried.…”

mentioning

confidence: 99%

Effect of the cross linkers’ concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase)

CheMan

Illias

Sulaiman

et al. 2017

Chem. Eng. Res. Bull.

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“…It has a high hydrophilicity, an important stability during long periods of repeated use and an easy application technique (Chu et al 2009). The cotton (Gossypium barbadence) employed for immobilization of E. coli pAD26 cells to produce CGTase enzyme was purchased from King Flex laboratory.…”

Section: Microbial Cells Immobilization and Fermentation Conditionsmentioning

confidence: 99%

“…However, biomaterials are renewable, nontoxic to microbial cells and especially to the environment, biodegradable, readily available, inexpensive and easy to use (Chu et al 2009;Pazzetto et al, 2011). Previous studies of our research group on CGTases have been focused on isolation of the microorganism, purification and characterization of Paenibacillus pabuli US132 CGTase (Jemli et al 2007), mutations affecting the enzyme activity (Jemli et al 2012) as well as overexpression of P. pabuli extracellular US132 CGTase gene in Escherichia coli and optimization of the culture media for recombinant enzyme production .…”

Section: Introductionmentioning

confidence: 99%

Improvement of cyclodextrin glycosyltransferase (CGTase) production by recombinant Escherichia coli pAD26 immobilized on the cotton

et al. 2012

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Abstract:The cyclodextrin glycosyltransferase (CGTase) of the recombinants Escherichia coli pAD26 cells immobilized on cotton was optimally produced by statistical methodology. Primarily, carbon and nitrogen sources were selected by one-factor-at-a-time method. Wheat starch, Casamino acid, Edamin and Hy-soy were identified as the best nutrients. These sources were secondly confirmed by Plackett-Burman design (fifteen variables were studied with sixteen experiments), as the most significant components with respect to CGTase production. In the third step, concentration of most significant factors and their interaction were optimized with a Box-Behnken experimental design. Under the optimized conditions (agitation 200 rpm, yeast extract concentration 20 g/L, wheat starch concentration 10 g/L and Hy-soy concentration 2.5 g/L), CGTase yield 145.11 U/mL was 3.6 and 23 folds higher than those obtained by the use of the initial conditions (39.77 U/mL) and free cells (6.37 U/mL), respectively.

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“…In contrast to ceramics, wood chips and straw, fibrous matrices provide adequate supporting surfaces for cell adsorption [9,10], due to their high specific surface area, void volume, mechanical and permeability, low pressure drop, diffusion problems and toxicity, maximum loading, biodegradability and durability and low cost and high availability [11][12][13]. Their natural configuration also allows them to trap more cells than other materials [12,14,15].…”

Section: Introductionmentioning

confidence: 99%

Surface Modifications for Controlled and Optimized Cell Immobilization by Adsorption: Applications in Fibrous Bed Bioreactors Containing Recombinant Cells

Kilonzo¹

2012

J Microb Biochem Technol

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The effects of surface properties of S. cerevisiae strains 468/pGAC9 and 468 on adhesion to polyethylenimine (PEI) and/glutaraldehyde (GA) pretreated cotton (CT), polyester (PE), polyester + cotton (PECT), nylon (NL), polyurethane foam (PUF), and cellulose re-enforced polyurethane (CPU) fibers were investigated. Process parameters (circulation velocity, pH, ionic strength, media composition and surfactants) were also examined. 80, 90, and 35% of the cells were adsorbed onto unmodified CT, PUF and PE, respectively. PEI-GA pre-treated CT and alkali treated PE yielded 25% and 60% cell adhesion, respectively. Adsorption rate (K a ) ranged from 0.06 to 0.17 for CT and 0.06 to 0.16 for PE at varied pH. Adhesion increased by 15% in the presence of ethanol, low pH and ionic strength, and decreased by 23% in the presence of yeast extract and glucose. Shear flow and 1% Triton X-100 detached 62 and 36% nonviable cells from PE and CT, respectively, suggesting that cell immobilization in fibrousbed bioreactors can be controlled to optimize cell density for long-term stability.

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Immobilization of bioluminescent Escherichia coli cells using natural and artificial fibers treated with polyethyleneimine

Cited by 51 publications

References 41 publications

Effect of the cross linkers’ concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase)

Effect of the cross linkers’ concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase)

Improvement of cyclodextrin glycosyltransferase (CGTase) production by recombinant Escherichia coli pAD26 immobilized on the cotton

Surface Modifications for Controlled and Optimized Cell Immobilization by Adsorption: Applications in Fibrous Bed Bioreactors Containing Recombinant Cells

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