Immobilization of bovine serum albumin via mussel-inspired polydopamine coating on electrospun polyethersulfone (PES) fiber mat for effective bilirubin adsorption

Wu, Keke; Song, Xingguo; Cui, Siyuan; Li, Zhentao; Jiao, Yanpeng; Zhou, Changren

doi:10.1016/j.apsusc.2018.04.242

Cited by 45 publications

(26 citation statements)

References 68 publications

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“…, bilirubin, with multiple binding sites per BSA molecule ( Blauer and King, 1970 ), as well as for other substances such as drugs ( Bi et al, 2009 ), or toxic compounds ( Das et al, 2017 ). Hence, BSA has been immobilized and used as a functional biopolymer for various applications, e.g., 1) for the improvement of blood compatibility (hemocompatibility) in blood filtration systems ( Zhang et al, 2013 ); 2) for the reduction of hemolysis in blood storage devices ( Li et al, 2014 ); 3) for the adsorption of bilirubin in the treatment of the liver disease hyperbilirubinemia via hemoperfusion ( Wu et al, 2018 ); 4) for the removal of bacteria, dyes and proteins by water filtration membranes ( Ahmad Khairuddin et al, 2018 ); or 5) for the generation of high-speed humidity sensors in conjugation with multiwall carbon nanotubes ( Bhattacharya and Sasmal, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Highly Efficient One-Step Protein Immobilization on Polymer Membranes Supported by Response Surface Methodology

et al. 2022

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Immobilization of proteins by covalent coupling to polymeric materials offers numerous excellent advantages for various applications, however, it is usually limited by coupling strategies, which are often too expensive or complex. In this study, an electron-beam-based process for covalent coupling of the model protein bovine serum albumin (BSA) onto polyvinylidene fluoride (PVDF) flat sheet membranes was investigated. Immobilization can be performed in a clean, fast, and continuous mode of operation without any additional chemicals involved. Using the Design of Experiments (DoE) approach, nine process factors were investigated for their influence on graft yield and homogeneity. The parameters could be reduced to only four highly significant factors: BSA concentration, impregnation method, impregnation time, and electron beam irradiation dose. Subsequently, optimization of the process was performed using the Response Surface Methodology (RSM). A one-step method was developed, resulting in a high BSA grafting yield of 955 mg m−2 and a relative standard deviation of 3.6%. High efficiency was demonstrated by reusing the impregnation solution five times consecutively without reducing the final BSA grafting yield. Comprehensive characterization was conducted by X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), and measurements of zeta potential, contact angle and surface free energy, as well as filtration performance. In addition, mechanical properties and morphology were examined using mercury porosimetry, tensile testing, and scanning electron microscopy (SEM).

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Section: Introductionmentioning

confidence: 99%

Highly Efficient One-Step Protein Immobilization on Polymer Membranes Supported by Response Surface Methodology

et al. 2022

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“…c) Bilirubin adsorption isotherms for iPAF‐5 and iPAF‐6 ( C 0 = 35−500 mg L −1 , m/V = 0.25 g L −1 ). d) Comparison of bilirubin maximum adsorption capacity ( q m ) and equilibrium adsorption time for our prepared adsorbents with other reported novel bilirubin adsorbents, PDA‐OMC, [ 4a ] HMCSs, [ 18 ] CS/SiO 2 ‐GO, [ 6e ] BSA‐PES, [ 19 ] 3D sponge, [ 20 ] MWCNTs, [ 21 ] CS/GO, [ 4b ] β ‐CD‐PES, [ 6a ] silicas, [ 6d ] macromesoporous rGO, [ 6c ] HPCMs, [ 22 ] imprinted PES fibers, [ 23 ] PEI‐PAN fibers, [ 6b ] heparin‐CS/GO, [ 24 ] rGO/CS, [ 25 ] rGO@HepMBm, [ 5b ] and PVA‐co‐PE. [ 26 ] e) Density functional theory (DFT) optimized adsorption complexes of bilirubin with PAF‐uc, iPAF, and iPAF‐6, and their calculated binding energies.…”

Section: Figurementioning

confidence: 99%

Porous Aromatic Framework with Tailored Binding Sites and Pore Sizes as a High‐Performance Hemoperfusion Adsorbent for Bilirubin Removal

Zhao

Cui

et al. 2020

Advanced Science

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Highly efficient removal of bilirubin from blood by hemoperfusion for liver failure therapy remains a challenge in the clinical field due to the low adsorption capacity and slow adsorption kinetics of currently used bilirubin adsorbents (e.g., activated carbon and ion-exchange resin). Recently, porous aromatic frameworks (PAFs) with high surface areas, tunable structures, and remarkable stability provide numerous possibilities to obtain satisfying adsorbents. Here, a cationic PAF with more mesopores, named iPAF-6, is successfully constructed via a de novo synthetic strategy for bilirubin removal. The prepared iPAF-6 exhibits a record-high adsorption capacity of 1249 mg g −1 and can adsorb bilirubin from 150 mg L −1 to normal concentration in just 5 min. Moreover, iPAF-6 shows a removal efficiency of 96% toward bilirubin in the presence of 50 g L −1 bovine serum albumin. It is demonstrated that positively charged aromatic frameworks and large pore size make a significant contribution to its excellent adsorption ability. More notably, iPAF-6/polyethersulfone composite fibers or beads are fabricated for practical hemoperfusion adsorption, which also show better removal performance than commercial adsorbents. This work can offer a new possibility for designing PAF-based bilirubin adsorbents with an appealing application prospect. Bilirubin is the major product of hemoglobin metabolism and is transported by albumin to liver for excretion. [1] The normal total bilirubin level in serum is 0.1−1.0 mg dL −1. [2] However, people with liver failure cannot eliminate bilirubin timely, leading to the excess accumulation of bilirubin in blood, called hyperbilirubinemia. [3] The extra bilirubin can cause yellow discoloration of skin and also bring about series of injuries to tissues and organs, and even result in death. [4] Therefore,

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“…[21] The presence of additional polydopamine layer was confirmed by appearance of a broad absorption band at 3300 cm -1 which corresponds to the stretching vibrations of -OH (phenolic hydroxyl) and N-H groups of polydopamine. [25] Treatment the carrageenan gel with PAMAM followed by polydopamine provided not only reactive functional groups for enzyme immobilization but also improved the mechanical properties of the coated gel disks which is necessary to keep the gel disk intact during the repeated usage. As shown from the compression test illustrated in Figure 3, the pristine carrageenan gel disk (Cgel) resisted the applied load up to a value of 1 N leading to a displacement of 4 mm.…”

Section: Matrix Preparation and Characterizationmentioning

confidence: 99%

Immobilization of β-galactosidase on carrageenan gel via bio-inspired polydopamine coating

Yassin

Naguib

Rehim

et al. 2018

J.Text.color. pol. sci.

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I N THE CONTEXT of diligent efforts directed to develop new polymeric matrices for enzyme immobilization, we developed carrageenan gel disks activated with bio-inspired polydopamine layer. The carrageenan gel disks were firstly treated with polycationic polymer (hyperbranched polyamidoamine) followed by a polydopamine layer. Such layer can act as an active platform for the covalent immobilization of enzymes via Michael addition and Schiff base. The mechanical strength of the coated gel disks was obviously enhanced. β-galactosidase was successfully immobilized with loading efficiency of about 52 %. Furthermore, the immobilized β-galactosidase exhibited enhanced activity at broader range of pH and temperature. Additionally, the immobilized β-galactosidase preserved around 80 % of the initial activity during the eleven reusability cycles. Generally, activation of the gel disks with the bioinspired polydopamine layer considered as a save alternative to the glutaraldehyde due to its cytotoxicity. Accordingly, the easily formed carrageenan gel disks coated with polydopamine could have potential as enzyme carrier in drug and food industries.

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Immobilization of bovine serum albumin via mussel-inspired polydopamine coating on electrospun polyethersulfone (PES) fiber mat for effective bilirubin adsorption

Cited by 45 publications

References 68 publications

Highly Efficient One-Step Protein Immobilization on Polymer Membranes Supported by Response Surface Methodology

Highly Efficient One-Step Protein Immobilization on Polymer Membranes Supported by Response Surface Methodology

Porous Aromatic Framework with Tailored Binding Sites and Pore Sizes as a High‐Performance Hemoperfusion Adsorbent for Bilirubin Removal

Immobilization of β-galactosidase on carrageenan gel via bio-inspired polydopamine coating

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