Immobilization of cells and enzymes to LentiKats®

Abstract

Biocatalyst immobilization is one of the techniques, which can improve whole cells or enzyme applications. This method, based on the fixation of the biocatalyst into or onto various materials, may increase robustness of the biocatalyst, allows its reuse, or improves the product yield. In recent decades, a number of immobilization techniques have been developed. They can be divided according to the used natural or synthetic material and principle of biocatalyst fixation in the particle. One option, based on the entrapment of cells or enzymes into a synthetic polyvinyl alcohol lens with original shape, is LentiKats® immobilization. This review describes the preparation principle of these particles and summarizes existing successful LentiKats® immobilizations. In addition, examples are compared with other immobilization techniques or free biocatalysts, pointing to the advantages and disadvantages of LentiKats®.

Co-Immobilization of Ketoreductase and Glucose Dehydrogenase

“…[13]; however, the co-immobilization of redox enzymes linked with cofactor regeneration have not been studied to date. Scale-up process of immobilization into PVA gel is also possible and was reviewed recently [22].…”

Optoregulated Protein Release from an Engineered Living Material

“…This in turn results in lower costs for culturing and processing them into living materials. In fact, bacteria have been successfully encapsulated in a variety of synthetic matrices made of alginate, polyacrylamide, polyvinyl alcohol, gelatin, agarose, etc., for applications in biocatalysis and probiotic food processing . However, the incorporation of programmed bacteria into biomaterials for envisioned applications in the fields of regenerative medicine and drug delivery has just started to be explored .…”

Intensified crude glycerol conversion to butanol by immobilized Clostridium pasteurianum

“…Immobilization is based on the fixation of biocatalysts into or onto various materials, for example natural wooden scobs, gelatine, agarose or synthetic polyurethane, polyacrylamide or polyvinyl alcohol (PVA) [14]. The resulting improvements of the bioprocess can include biocatalyst recycling in a repeated batch or continuous processes, or the protection of the biocatalyst against environmental effects [15][16][17].…”

“…In this work, entrapment into PVA particles was tested [14]. PVA is a very suitable entrapment material because of its high tolerance towards a wide range of temperatures (10-50 °C) [22] and pHs (2.3-9.0) [23,24].…”