Immobilization of cross-linked tannase enzyme on multiwalled carbon nanotubes and its catalytic behavior

Ong, Chong-Boon; Annuar, Mohamad Suffian Mohamad

doi:10.1080/10826068.2018.1425707

Cited by 24 publications

(7 citation statements)

References 27 publications

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“…The cycling stability of mGP 3 H 2 -Tannase is superior to that of many supports for tannase immobilization (Table ). ,,,, The FTIR spectra and TG curves of mGP 3 H 2 -Tannase after 10 cycles are shown in Figures S18 and S19 (Supporting Information). The slight changes indicate the stable structure of mGP 3 H 2 -tannase.…”

Section: Resultsmentioning

confidence: 99%

“…Despite the previously mentioned benefits, the enzyme-catalyzed transformation of tannin using tannase still faces several key challenges. For example, the complicated purification process, high production cost, insufficient enzyme stability, limited enzyme selectivity or specificity, low tolerance in the presence of inhibitors, finite application conditions, and inability to recycle after every use of free forms make this approach unsustainable for the long-term usage in industrial applications. , In this situation, immobilization of enzymes on a solid support is regarded as an effective strategy to overcome the above-mentioned limitations. − Stable supports for tannase immobilization include calcium alginate beads, − chitosan, manganese nanoparticles, magnetic nanoparticles, multiwalled carbon nanotubes, and silica oxide nanoparticles . The immobilization yield and reusability have been improved to a certain extent, but some major drawbacks remain.…”

Section: Introductionmentioning

confidence: 99%

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Construction of a Tannase-Immobilized Magnetic Graphene Oxide/Polymer Nanobiocatalyst with Enhanced Enzyme Stability for High-Efficiency Transformation of Tannins

Huan

Liu

et al. 2022

ACS Sustainable Chem. Eng.

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Highly efficient biotransformation of natural compounds into sustainable biochemical products has attracted great attention. The integration of nanoscience and biotechnology provides attractive solutions for this purpose. Herein, we report the fabrication of a nanobiocatalyst employing a magnetic graphene oxide/polymer nanocomposite as a robust carrier for immobilizing the tannase enzyme, which catalyzes the bioconversion of tannin into gallic acid and glucose. Attributed to the covalent immobilization and propitious interface properties of the coated polymers comprising polyethylenimine and sodium hyaluronate, the nanobiocatalyst is stable without compromising the enzymatic activity. The nanobiocatalyst exhibits 91.8% activity of original tannase and a high enzyme bound amount of 356.8 mg g −1 . The stability tests at variable temperatures (30−80 °C) and under pH conditions (4.0−9.0), various inhibitors, and a long-term storage process (25 days) reveal that the heterofunctional support and surface microenvironment facilitate better stability, adaptability, and tolerance ability of the nanobiocatalyst modified with a multicomponent polymer in comparison to the free enzyme and the nanobiocatalyst modified with the monocomponent polyethylenimine. The nanobiocatalyst maintains 94.2% of its initial activity after 10 consecutive uses and is 100% recoverable by applying an external magnet. Moreover, the nanobiocatalyst is used to hydrolyze 96.5 and 95.1% tannins in extracts from Chinese Torreya grandis testa and cake, respectively. These results establish the practicability of magnetic graphene oxide-based supports for immobilizing tannase and the promising application of immobilized tannase for efficient tannin hydrolysis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Construction of a Tannase-Immobilized Magnetic Graphene Oxide/Polymer Nanobiocatalyst with Enhanced Enzyme Stability for High-Efficiency Transformation of Tannins

Huan

Liu

et al. 2022

ACS Sustainable Chem. Eng.

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“…Due to their biochemical properties, the demand for industrial enzymes is increasing, asking for the (Ong and Annuar et al 2018) and enhances the enzymatic stability, enlarging the pH and temperature range of action (Yu et al 2004;Schons et al 2011;Kumar et al 2015). Immobilization can also protect the enzymes from inhibition effects played by metal ions.…”

Section: Tannasesmentioning

confidence: 99%

Biotransformation of industrial tannins by filamentous fungi

Prigione

Spina

Tigini

et al. 2018

Appl Microbiol Biotechnol

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Tannins are secondary metabolites widely distributed in the plant kingdom. They act as growth inhibitors towards many microorganisms: upon microbial attack, they are released helping to fight the infection of plant tissues. Extraction of tannins from plants is an active industrial sector with several applications from the beginning of the industrial era. Actually, tannins have many industrial applications in oenology, animal feeding, mining and chemical industry and, in particular, in the tanning industry. But tannins are also considered very recalcitrant pollutants in wastewaters of different origins. The ability to grow on plant substrates rich in tannins and on industrial tannin preparations is traditionally considered peculiar of some species of fungi that have developed mechanisms to tolerate the toxicity of tannins producing a complex enzymatic pattern active in the transformation of these substrates, mainly by hydrolysis and oxidation. Filamentous fungi capable of degrading tannins could have a strong environmental impact as bioremediation agents mostly in the treatment of tanning wastewaters.

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“…Reuse promotes savings in enzyme, time, investment, and labor ( Andrade et al, 2020 ). These improved properties allow the use of derivatives in a packed bed reactor (PBR) ( Wu et al, 2016 ; Ong and Annuar, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Immobilization of the Tannase From Aspergillus fumigatus CAS21: Screening the Best Derivative for the Treatment of Tannery Effluent Using a Packed Bed Reactor

Cavalcanti

Maestrello

Guimarães³

2021

Front. Bioeng. Biotechnol.

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Enzyme immobilization is an important alternative to stabilize enzyme properties favoring the efficiency of derivatives (enzyme + support/matrix) for different purposes. According to this, the current study aimed to immobilize the Aspergillus fumigatus CAS21 tannase and the use of the derivatives in the treatment of the effluent produced by the tannery industry. The tannase was immobilized on sodium alginate, DEAE-Sephadex, amberlite, and glass pearls as supports. Calcium alginate was the most adequate support for tannase immobilization with 100% yield and 94.3% for both efficiency and activity. The best tannase activity for the calcium alginate derivative was obtained at 50°C–60°C and pH 5.0. Thermal and pH stabilities evaluated for 24 h at 30°C–60°C and pH 4–7, respectively, were improved if compared to the stability of the free enzyme. Considering the reuse of the calcium alginate derivative, 78% of the initial activity was preserved after 10 catalytic cycles, and after the 9-month storage at 4°C, the activity was maintained in 70%. This derivative was applied in a packed bed reactor (PBR) for the treatment of tannin-rich effluents from the tannery industry. The reduction of the tannin content was effective reaching degradation of 74–78% after 48 h of PBR operation. The concentration of total phenolic compounds was also reduced, and the color and clarity of the effluent improved. In conclusion, the calcium alginate derivative is an attractive alternative as biocatalyst for large-scale treatment of the effluents from the tannery industry.

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Immobilization of cross-linked tannase enzyme on multiwalled carbon nanotubes and its catalytic behavior

Cited by 24 publications

References 27 publications

Construction of a Tannase-Immobilized Magnetic Graphene Oxide/Polymer Nanobiocatalyst with Enhanced Enzyme Stability for High-Efficiency Transformation of Tannins

Construction of a Tannase-Immobilized Magnetic Graphene Oxide/Polymer Nanobiocatalyst with Enhanced Enzyme Stability for High-Efficiency Transformation of Tannins

Biotransformation of industrial tannins by filamentous fungi

Immobilization of the Tannase From Aspergillus fumigatus CAS21: Screening the Best Derivative for the Treatment of Tannery Effluent Using a Packed Bed Reactor

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