Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst

Abstract

The immobilization technology provides a potential pathway for enzyme recycling. Here, we evaluated the potential of using dextranase immobilized onto hydroxyapatite nanoparticles as a promising inorganic material. The optimal immobilization temperature, reaction time, and pH were determined to be 25 °C, 120 min, and pH 5, respectively. Dextranase could be loaded at 359.7 U/g. The immobilized dextranase was characterized by field emission gun-scanning electron microscope (FEG-SEM), X-ray diffraction (XRD), and Fourier-transformed infrared spectroscopy (FT-IR). The hydrolysis capacity of the immobilized enzyme was maintained at 71% at the 30th time of use. According to the constant temperature acceleration experiment, it was estimated that the immobilized dextranase could be stored for 99 days at 20 °C, indicating that the immobilized enzyme had good storage properties. Sodium chloride and sodium acetic did not desorb the immobilized dextranase. In contrast, dextranase was desorbed by sodium fluoride and sodium citrate. The hydrolysates were 79% oligosaccharides. The immobilized dextranase could significantly and thoroughly remove the dental plaque biofilm. Thus, immobilized dextranase has broad potential application in diverse fields in the future.

Immobilization of Dextranase Obtained from the Marine Cellulosimicrobium sp. Y1 on Nanoparticles: Nano-TiO2 Improving Hydrolysate Properties and Enhancing Reuse

“…With the adsorption of the enzyme at 1377.83 cm −1 and at 1299.89 cm −1 , a new band appeared that belongs to the C–O and C–N stretching vibrations. This result indicated that a new carboxylic acid group was generated by enzyme immobilization [ 12 , 53 ]. Omnic 9.2 software was used to plot FT–IR spectrum from 800 cm −1 to 2000 cm −1 , and the vibration band in the spectrum can be seen more clearly.…”

“…Immobilization of enzymes improves their applicability [ 8 ] and storage [ 9 ]. Several carriers, such as alginate [ 10 ], chitin [ 11 ], agarose [ 1 ], and hydroxyapatite [ 12 ], have been used to immobilize dextranase. Although the immobilization rate of the immobilized dextranase has been improved, the stability and reusability of the immobilized enzymes are still not adequate for practical applications; therefore, identifying suitable immobilized materials continues to be the focus of research [ 13 , 14 ].…”

Cloning of Cold-Adapted Dextranase and Preparation of High Degree Polymerization Isomaltooligosaccharide

“…PsDex1711 is an endodextranase that hydrolyzes the α- (1,6) bond in dextran from the inside to reduce its molecular weight. In contrast, exodextranase cut outward from the reducing end to release glucose [31]. Most dextran anhydrases reported are endotangent dextran anhydrases, and the product formed (i.e., IMOs) has a crucial application value.…”

Synthesis and evaluation of nanosystem containing chondroitinase ABCI based on hydroxyapatite

“…The anti-inflammatory drug ibuprofen, the antibiotics norfloxacin and vancomycin, the bisphosphonate alendronate, the cardiovascular drug carvedilol, the anticancer drug cis-diamminedichloroplatinum (II), the di(ethylenediamineplatinum) medronate, and others have all been carried by nano-hydroxyapatite (Nur Farahiyah et al 2014 ). Additionally, nano-hydroxyapatite was used to effectively stabilize these enzymes with a straightforward uptake protocol while maintaining their catalytic activity for the immobilization of β-glucosidase, protease, and phytase with industrial applications as well as dextranase, which is involved in the breakdown of dental plaque (Coutinho et al 2018 ; Zdarta et al 2015 ; Coutinho et al 2020 ; Ding et al 2020 ). A different study used the hydroxyapatite drug composition to release amoxicillin to treat bone infections (Prasanna et al 2018 ).…”