Immobilization of diesel-degrading consortia for bioremediation of diesel-contaminated groundwater and seawater

Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria

“…This might be due to the challenges of applying free cells in open waters or coastal areas. To cope with that, some researchers propose immobilizing the bacterial agents into carriers to enhance their biodegradation efficiency [28,30,31]. For instance, Junusmin et al [64] observed that a freeze-dried bacterial consortium (composed of two species of Bacillus and one Enterobacter species) immobilized onto different carriers could degrade up to 93% TPHs after 28 days incubation with crude oil.…”

“…Regarding the application of bioremediation agents in open water systems, such as seawater, the application of free-cell bioremediation might be challenging as it might disperse in the water column [26]. To tackle this issue, some studies suggest the application of immobilized bacterial cells into carriers, biofilms or enzyme substances to enhance the hydrocarbons biodegradation performance [27][28][29][30][31]. Wang et al [28] studied the application of immobilized bacteria in field tests (3 months), contaminated with crude oil and diesel and observed that the immobilized bacterial consortia performed better in the degradation of hydrocarbons.…”

Bioremediation of tetracycline antibiotics-contaminated soil by bioaugmentation

“…On the one hand, this could be due to the excellent structure of SCB, which contributed to the adhesion and proliferation of microbes on the SCB, resulting in an outstanding mass transfer performance, enhanced cell permeability, and high biological density. 61,62 On the other hand, the porous SCB was capable of providing an ideal shelter and biocompatible environment for the introduced microorganisms and for partially resisting the adverse effects of substrate OTC inhibition, 66 and its decomposition that supplied carbon and nitrogen could increase soil nutrients so as to create a condition that was favorable for the growth of the introduced microorganisms. 60,61 Moreover, in terms of the bioprocess mediated by the freely suspended cells or immobilized cells, the degradation rate reached a maximum of 4.27 mg kg −1 d −1 and 4.75 mg kg −1 d −1 on the 5 th d post-inoculation, respectively, and gradually reduced as the incubation time increased, which revealed that the bacterium BC could rapidly adapt to the OTC-contaminated soil and had potential for the remediation of OTC-contaminated soils.…”

“…54 The immobilized process could offer substantial advantages in terms of the enhanced cell permeability, enhanced surface area, high biological density, increased microbial stability, environmental sustainability, strong resistance to toxicity, high tolerance to the targeted contaminants' toxicity, enhanced removal efficiency of the targeted contaminants, protection of the introduced microorganisms, and broader operational range of temperature and pH. 60–64 Interestingly, among various immobilization support matrix, sugarcane bagasse (SCB) has the advantages of appropriate porous properties, low-price, abundance, non-toxicity, high affinity to microorganisms, high mechanical strength, and easy biodegradability. 45 Simultaneously, it is also capable of supplying essential organics and nutrients so as to support bacterial growth.…”

Characteristics of crude oil‐degrading bacteria Gordonia iterans isolated from marine coastal in Taean sediment

“…Gordonia sp. JG39 was reduced in freshwater with 500 mg‐ diesel oil/L, 94% of diesel degraded within 11 days (Chen, Whang, Pan, & Yang, ).…”