Immobilization of double functionalized carbon nanotubes on glassy carbon electrodes for the electrochemical sensing of the biotin–avidin affinity

Fabre, Bruno; Samorì, Cristian; Bianco, Alberto

doi:10.1016/j.jelechem.2011.11.029

Cited by 10 publications

(3 citation statements)

References 55 publications

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“…The unique feature of TMTAA makes it a suitable candidate as tethering agent for biomolecule immobilization in biosensor applications [60]. Ferrocene functionalized CNT was also studied and electrochemical systems for biosensor applications [80,81,82]. Yang et al first reported the preparation of non-covalent nanohybrid of ferrocene functionalized SWCNT with enhanced electrochemical properties [62].…”

Section: Functionalization Of Carbon Nanotubesmentioning

confidence: 99%

Non-Covalent Functionalization of Carbon Nanotubes for Electrochemical Biosensor Development

Zhou

Fang

Ramasamy

2019

Sensors

257

127

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Carbon nanotubes (CNTs) have been widely studied and used for the construction of electrochemical biosensors owing to their small size, cylindrical shape, large surface-to-volume ratio, high conductivity and good biocompatibility. In electrochemical biosensors, CNTs serve a dual purpose: they act as immobilization support for biomolecules as well as provide the necessary electrical conductivity for electrochemical transduction. The ability of a recognition molecule to detect the analyte is highly dependent on the type of immobilization used for the attachment of the biomolecule to the CNT surface, a process also known as biofunctionalization. A variety of biofunctionalization methods have been studied and reported including physical adsorption, covalent cross-linking, polymer encapsulation etc. Each method carries its own advantages and limitations. In this review we provide a comprehensive review of non-covalent functionalization of carbon nanotubes with a variety of biomolecules for the development of electrochemical biosensors. This method of immobilization is increasingly being used in bioelectrode development using enzymes for biosensor and biofuel cell applications.

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Section: Functionalization Of Carbon Nanotubesmentioning

confidence: 99%

Non-Covalent Functionalization of Carbon Nanotubes for Electrochemical Biosensor Development

Zhou

Fang

Ramasamy

2019

Sensors

257

127

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“…Yet, direct comparisons with other materials applied to the same type of sensors ought to be done, considering the same target analyte. In this sense, regardless of the existence of several reports on the detection and quantification of avidin via affinity interaction with biotin, − none are based on impedimetric methods but rather on voltammetric and transistor approaches, for which the reported LODs and LOQs are generally of the same order of magnitude as the ones found in this work.…”

Section: Results and Discussionmentioning

confidence: 66%

Physical Structure and Electrochemical Response of Diamond–Graphite Nanoplatelets: From CVD Synthesis to Label-Free Biosensors

Santos¹,

Pereira²,

Fernandes³

et al. 2019

ACS Appl. Mater. Interfaces

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Hybrid diamond–graphite nanoplatelet (DGNP) thin films are produced and applied to label-free impedimetric biosensors for the first time, using avidin detection as a proof of concept. The DGNPs are synthesized by microwave plasma chemical vapor deposition through H2/CH4/N2 gas mixtures in a reproducible and rapid single-step process. The material building unit consists of an inner two-dimensional-like nanodiamond with preferential vertical alignment covered by and covalently bound to nanocrystalline graphite grains, exhibiting {111}diamond||{0002}graphite epitaxy. The DGNP films’ morphostructural aspects are of interest for electrochemical transduction, in general, and for Faradaic impedimetric biosensors, in particular, combining enhanced surface area for biorecognition element loading and facile Faradaic charge transfer. Charge transfer rate constants in phosphate buffer saline/[Fe(CN)6]4– solution are shown to increase up to 5.6 × 10–3 cm s–1 upon N2 addition to DGNP synthesis. For the impedimetric detection of avidin, biotin molecules are covalently bound as avidin specific recognition elements on (3-aminopropyl)triethoxysilane-functionalized DGNP surfaces. Avidin quantification is attained within the 10–1000 μg mL–1 range following a logarithmic dependency. The limits of detection and of quantitation are 1.3 and 6.4 μg mL–1 (19 and 93 nM), respectively, and 2.3 and 13.8 μg mL–1 (33 and 200 nM) when considering the nonspecific response of the sensors.

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“…Herein we detect concentrations down to 2.5 ng ml −1 in both modalities, but these are not limit values. As comparison, electrochemical sensing with carbon nanotubes can reach 0.9-20 nM [45] and functionalized glassy carbon electrode 10 nM [46]. Electrical conductivity detection based on graphene field effect transistors has been reported to detect less than 50 ng ml −1 concentrations of streptavidin.…”

Section: Effect On Electrical Properties Of Graphenementioning

confidence: 99%

Electrochemical functionalization strategy for chemical vapor deposited graphene on silicon substrates: grafting, electronic properties and biosensing

Sarmiento¹,

Guzmán²,

Lockett³

et al. 2019

Nanotechnology

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Herein, we present an electrochemical functionalization strategy for high quality single-layer and multilayer chemical vapor deposited (CVD) graphene directly on a Si/SiO 2 chip facilitating electronic interfacing. This method avoids oxidation and tearing of graphene basal planes. We demonstrate effective functionalization by D-(+)-biotin (Bio), 4-(phenyldiazenyl)-aniline (Dz), and gallic acid (Gall) using cyclic voltammetry. Raman spectroscopy and XPS are used to demonstrate effective functionalization. In order to evaluate the effect of the electrochemical functionalization on graphene properties, DC electrical conductivity, XPS, mobility, and carrier density analysis are presented. We show that this functionalization strategy does not degrade graphene mobility (10 3 cm 2 V −1 s −1 ). After functionalization we observe a rise in Fermi level of ∼0.06 eV. In addition, we prove sensing capabilities with a CVD graphene monolayer on the biotin/avidin system by electrical resistance measurements and electrochemical impedance spectroscopy reaching a detection of 2.5 ng ml −1 . This paper demonstrates an effective strategy to functionalize high quality CVD graphene on a chip compatible with an electronic interface readout.

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Immobilization of double functionalized carbon nanotubes on glassy carbon electrodes for the electrochemical sensing of the biotin–avidin affinity

Cited by 10 publications

References 55 publications

Non-Covalent Functionalization of Carbon Nanotubes for Electrochemical Biosensor Development

Non-Covalent Functionalization of Carbon Nanotubes for Electrochemical Biosensor Development

Physical Structure and Electrochemical Response of Diamond–Graphite Nanoplatelets: From CVD Synthesis to Label-Free Biosensors

Electrochemical functionalization strategy for chemical vapor deposited graphene on silicon substrates: grafting, electronic properties and biosensing

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