Immobilization of endo-inulinase on poly-d-lysine coated CaCO3 micro-particles

Karimi, Mahsan; Habibi-Rezaei, Mehran; Safari, Mohammad; Moosavi-Movahedi, Ali Akbar; Sayyah, Maryam; Sadeghi, Rohollah; Kokini, Jozef L.

doi:10.1016/j.foodres.2014.08.041

Cited by 23 publications

(6 citation statements)

References 50 publications

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“…Thus, Ghada et al., 2017 reported that the half‐lives and D values of inulinase after covalent immobilization indicated better thermal stability of the immobilized inulinase. Karimi et al., 2014b have shown that the immobilization of endo‐inulinase increases thermal optimum of the enzyme allowing the reaction to be conducted at higher temperatures. The high temperature regime of operation aids to prevent microbial contamination and allows better solubility of inulin as substrate.…”

Section: Resultsmentioning

confidence: 99%

“…The recovered activity was normalized taking into account the same amount of enzyme. The activity was also measured during preincubation at appropriate time intervals in order to estimate the thermal stability of the enzyme . The inactivation process follows the first order reaction kinetics; it is determined according to the equation .…”

Section: Methodsmentioning

confidence: 99%

“…Previously, inulinase has been immobilized on various supports, e.g. biopolymers (chitin, chitosan, alginate) [16][17][18][19][20], synthetic polymers (polyurethane foam, polyvinyl alcohol) [21,22], inorganic supports (MWCNT, silica nanoparticles) [3,[23][24][25], or hybrids consisting of organic and inorganic parts (poly-d-lysine coated CaCO 3 microparticles, magnetic nanoparticles with wheat gluten hydrolysates, magnetite chitosan microparticles) [26][27][28]. Generally speaking, nanomaterials are currently widely used for immobilization of proteins.…”

Section: Introductionmentioning

confidence: 99%

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Inulinase immobilization on polyethylene glycol/polypyrrole multiwall carbon nanotubes producing a catalyst with enhanced thermal and operational stability

Temkov

Petrovski

Gjorgieva

et al. 2019

Engineering in Life Sciences

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This paper describes the development of a simple method for mixed non‐covalent and covalent bonding of partially purified inulinase on functionalized multiwall carbon nanotubes (f‐MWCNTs) with polypyrrole (PPy). The pyrrole (Py) was electrochemically polymerized on MWCNTs in order to fabricate MWCNTs/PPy nanocomposite. Two multiple forms of enzyme were bound to N‐H functional groups from PPy and ‐COO− from activated MWCNTs to yield a stable MWCNTs/PPy/PEG immobilized preparation with increased thermal stability. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) were used to confirm functionalization of nanoparticles and immobilization of the enzyme. The immobilization yield of 85% and optimal enzyme load of 345 μg protein onto MWCNTs was obtained. The optimum reaction conditions and kinetic parameters were established using the UV‐Vis analytical assay. The best functional performance for prepared heterogeneous catalyst has been observed at pH 3.6 and 10, and at the temperatures of 60 and 80ºC. The half‐life (t1/2) of the immobilized inulinase at 60 and 80ºC was found to be 231 and 99 min, respectively. The reusability of the immobilized formulation was evaluated based on a method in which the enzyme retained 50% of its initial activity, which occurred after the eighteenth operation cycle.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inulinase immobilization on polyethylene glycol/polypyrrole multiwall carbon nanotubes producing a catalyst with enhanced thermal and operational stability

Temkov

Petrovski

Gjorgieva

et al. 2019

Engineering in Life Sciences

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“…The inclusion of inorganic fillers, such as ZnO, TiO 2 , SiO 2 , Al 2 O 3 , Mg(OH) 2 , and CaCO 3 as third constituent in FRPCs, improve the fiber‐matrix interface circumstances and also reduce porosity, brittle failure and misalignment of fibers . The appropriate size, shape, specific surface area, concentration, and distribution of fillers contribute to significant improvement in physical, mechanical, and thermal properties of hybrid particulate filled FRPCs .…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of novel zirconia filled glass fiber reinforced polyester hybrid composites

Munawar

Khan

Gull

et al. 2016

J of Applied Polymer Sci

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Novel hybrid glass fiber reinforced polyester composites (GFRPCs) filled with 1-5 wt % microsized zirconia (ZrO 2 ) particles, were fabricated by hand lay-up process followed by compression molding and evaluated their physical, mechanical and thermal behaviors. The consumption of styrene in cured GFRPCs was confirmed by Fourier transform infrared spectroscopy. The potential implementation of ZrO 2 particles lessened the void contents marginally and substantially enhanced the mechanical and thermal properties in the resultant hybrid composites. The GFRPCs filled with 4 wt % ZrO 2 illustrated noteworthy improvement in tensile strength (66.672 MPa) and flexural strength (67.890 MPa) while with 5 wt % ZrO 2 showed 63.93% rise in hardness, respectively, as compared to unfilled GFRPCs. Physical nature of polyester matrix for composites and an improved glass transition temperature (T g ) from 103 to 112 8C was perceived by differential scanning calorimetry thermograms. Thermogravimetric analysis revealed that the thermal stability of GFRPCs was remarkably augmented with the addition of ZrO 2 .

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“…Several carrier materials have already been examined for endoinulinase immobilisation, such as polystyrene, chitin and chitosan granules, activated alumina, anion-exchange resin (Yun et al 2000), poly-d-lysine coated CaCO 3 micro-particles (Karimi et al 2014b), functionalised silica nanoparticles (Karimi et al 2014a), carbon nanotubes (Garlet et al 2014), or alginate-chitosan beads (Missau et al 2014).…”

mentioning

confidence: 99%

Immobilisation of endoinulinase on polyhydroxybutyrate microfibers

Beran¹,

Pinkrová²,

Urban³

et al. 2016

Czech J. Food Sci.

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Beran M., Pinkrová J., Urban M., Drahorád J. (2016): Immobilisation of endoinulinase on polyhydroxybutyrate microfibres. Czech J. Food Sci., 34: 541-546.Due to the health benefits associated with the consumption of prebiotic short-chain fructooligosaccharides (sc-FOS) and inulooligosaccharides (IOS), there is increased interest in the use of these compounds in food products. We have developed a new biocatalyst for the production of FOS and IOS by inulin hydrolysis. Endoinulinase from Aspergillus niger Inulinase ® Novozym 960 (Novozymes) was immobilised on polyhydroxybutyrate (PHB) nanofibres and microfibres by hydrophobic interactions. The PHB fibres were prepared by centrifugal spinning. FOS and IOS profiles were determined by ion-exchange chromatography with the Rezex RSO-Oligosaccharide column. The biocatalyst had very good activity and stability after repeated applications. It can be used in biocatalytic membrane reactors for the production of prebiotic oligosaccharides.

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Immobilization of endo-inulinase on poly-d-lysine coated CaCO3 micro-particles

Cited by 23 publications

References 50 publications

Inulinase immobilization on polyethylene glycol/polypyrrole multiwall carbon nanotubes producing a catalyst with enhanced thermal and operational stability

Inulinase immobilization on polyethylene glycol/polypyrrole multiwall carbon nanotubes producing a catalyst with enhanced thermal and operational stability

Fabrication and characterization of novel zirconia filled glass fiber reinforced polyester hybrid composites

Immobilisation of endoinulinase on polyhydroxybutyrate microfibers

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