Immobilization of Enzymes on PLGA Sub‐Micrometer Particles by Crosslinked Layer‐by‐Layer Deposition

Sieber, Sandro; Siegrist, Stefan; Schwarz, Stéphanie; Porta, Fabiola; Schenk, Susanne; Huwyler, Jörg

doi:10.1002/mabi.201700015

Cited by 17 publications

(11 citation statements)

References 42 publications

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“…Zebrafish Experiments : Zebrafish larvae originating from adult Tg(mpeg1:Gal4;UAS:Kaede) were raised at 28 °C in zebrafish culture media . Pigment cell formation was prevented by the addition of 1‐phenyl‐2‐thiourea to the zebrafish culture media at 24 h post fertilization.…”

Section: Methodsmentioning

confidence: 99%

Poly(Sarcosine) Surface Modification Imparts Stealth‐Like Properties to Liposomes

Bleher

Buck

Muhl

et al. 2019

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Thereby, one of the main goals of liposomes is to protect the entrapped drug while also reducing its off-site toxicity, as shown for multiple formulations. [5][6][7][8] To this end, increasing on-target tissue concentration is a key aspect which can be achieved both by nontargeted and targeted liposomes. [9][10][11][12] In this regard, a crucial point is the prolongation of systemic circulation lifetime. It is well-known that upon injection the majority of conventional (plain) liposomes are cleared rapidly from the blood stream by cells of the mononuclear phagocyte system. [13][14][15] To enhance circulation times, a variety of approaches to modify the surface of liposomes emerged, [16][17][18] yet ultimately polyethylene glycol (PEG) established itself as gold standard in the early 1990s aiming at decreasing opsonization. [19][20][21] PEGylation not only reduces but also alternates the recognition of nanoparticles by complement factors and opsonins, resulting in a decreased nanoparticle recognition and clearance by macrophages. [22,23] Manipulating opsonization, including the reduction of complement activation thus is one of the main goals in preventing rapid clearance.Today, surface modifications of liposomes are mostly realized with amphiphilic lipid-polymer conjugates like 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-mPEG2k). [24,25] Despite the benefits and being considered mostly nonimmunogenic, phospholipid-PEG conjugates have also demonstrated some drawbacks over the Circulation lifetime is a crucial parameter for a successful therapy with nanoparticles. Reduction and alteration of opsonization profiles by surface modification of nanoparticles is the main strategy to achieve this objective. In clinical settings, PEGylation is the most relevant strategy to enhance blood circulation, yet it has drawbacks, including hypersensitivity reactions in some patients treated with PEGylated nanoparticles, which fuel the search for alternative strategies. In this work, lipopolysarcosine derivatives (BA-pSar, bisalkyl polysarcosine) with precise chain lengths and low polydispersity indices are synthesized, characterized, and incorporated into the bilayer of preformed liposomes via a post insertion technique. Successful incorporation of BA-pSar can be realized in a clinically relevant liposomal formulation. Furthermore, BA-pSar provides excellent surface charge shielding potential for charged liposomes and renders their surface neutral. Pharmacokinetic investigations in a zebrafish model show enhanced circulation properties and reduction in macrophage recognition, matching the behavior of PEGylated liposomes. Moreover, complement activation, which is a key factor in hypersensitivity reactions caused by PEGylated liposomes, can be reduced by modifying the surface of liposomes with an acetylated BA-pSar derivative. Hence, this study presents an alternative surface modification strategy with similar benefits as the established PEGylation of nanoparticles, but with the potential...

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Section: Methodsmentioning

confidence: 99%

Poly(Sarcosine) Surface Modification Imparts Stealth‐Like Properties to Liposomes

Bleher

Buck

Muhl

et al. 2019

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“…Eggs were collected and kept in zebrafish culture media containing 1-phenyl 2-thiourea (PTU) in order to suppress pigment cell formation as already described elsewhere. 59 Small clusters formed by DY-633-P20-a and SRB-P20-b, large clusters formed by DY-633-P20-a and SRB-P20-b, and non-clustered sample containing DY-633-P20-b and SRB-P20-b were injected into zebrafish blood circulation (3 nL, 0.2 mg mL −1 ) via the Duct of Cuvier at 2 days post fertilization (dpf ). 60 Injected live zebrafish embryos were casted into 0.3% agarose (w/v) containing 0.01% tricaine (w/v) as anesthetic.…”

Section: 6mentioning

confidence: 99%

DNA-directed arrangement of soft synthetic compartments and their behavior in vitro and in vivo

et al. 2020

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“…In vivo tests on mice with diabetes type I demonstrated that the nanoreactor carrier was effective for regulating the glycemia levels to normal values for more than 80 hours with a single administration. Another recent application was developed for potential uses for biomedical applications [81]. PLGA-enzyme particles were produced by immobilizing the enzymes acid phosphatase (AP) or β-galactosidase (β-GAL) between the PSS and PAH layers via glutaraldehyde cross-linking.…”

Section: Layer By Layer Assembliesmentioning

confidence: 99%

“…Another recent application was developed for potential uses for biomedical applications [81]. PLGA-enzyme particles were produced by immobilizing the enzymes acid phosphatase (AP) or β-galactosidase (β-GAL) between the PSS and PAH layers via glutaraldehyde cross-linking.…”

Section: Layer By Layer Assembliesmentioning

confidence: 99%

Polymer Capsules for Enzymatic Catalysis in Confined Environments

et al. 2018

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Catalysis is at the base of a series of biological and technological application processes. In recent years, the tendency has developed to carry out catalyzed reactions within confined structures, thus forming systems called micro or nanoreactors. Compartmentalized structures are cavities delimited by a wall where specific functions are introduced with a defined concentration and in the desired sites. These containers are generally referred to as nano or microcapsules, assuming the function of reactors in the presence of chemical reactions. Among the various types of existing structures, one of the most interesting is represented by systems made with polymers. This review aims to highlight some of the current advances in the use of functionalized structures that are useful for catalysis reactions, paying particular attention to polymer capsules and enzymes. The built-up methods used for the production of polymer capsules, as well as the aspects that influence membrane permeability and reactivity to environmental conditions, are discussed. Recent advances on biocatalysis confined in polymeric capsules are illustrated, and the strengths and weaknesses of the principal nanoreactors are considered.

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Immobilization of Enzymes on PLGA Sub‐Micrometer Particles by Crosslinked Layer‐by‐Layer Deposition

Cited by 17 publications

References 42 publications

Poly(Sarcosine) Surface Modification Imparts Stealth‐Like Properties to Liposomes

Poly(Sarcosine) Surface Modification Imparts Stealth‐Like Properties to Liposomes

DNA-directed arrangement of soft synthetic compartments and their behavior in vitro and in vivo

Polymer Capsules for Enzymatic Catalysis in Confined Environments

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